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MAGNETIC SEPARATORS
Spherotech has several different designs of magnetic
separators. These are used for separating both Paramagnetic and
Ferromagnetic particles from a suspension. These designs accommodate
different size tubes and other receptacles specific to various applications.
(A) The SPHEROTM FlexiMag Separator (Cat. No.: FMS-1000)
accommodates tubes ranging from 1.5 mL microfuge tube to 200 mL tissue
culture bottle. It uses interchangeable tube holders to secure different
size tubes and bottles. The separator comes with a set of three tube
holders, Small, Medium and Large. Additional holders can be purchased
separately, if needed.
a) The Small holder holds up to four 1.5 mL microfuge or
four 10 or 12x75 mm test tubes (8 total).
b) The Medium holder can hold two 15 mL centrifuge or two
16x100 mm tubes (4 total)
c) The Large holder can hold two 50 mL centrifuge tubes (4
total).
d) Two 200 mL tissue culture bottles directly without any
tube holders.
(B) The SPHEROTM FlexiMag Separator Jr. (Cat. No.:
FMJ-1000) holds up to eight 1.5 mL microfuge tubes, 5 mL cryovials, 10x75 mm
or 12x75mm tubes.
(C) The SPHEROTM MicroMag Separator (Cat No. MMS-2100 and
MMS-2000) are designed to fit any 96-well plate with round bottom, flat
bottom or V bottom.
(D) The SPHEROTM HandiMag Separator is a 1"x2"x0.375"
Neodymium-Iron-Boron high strength magnet with nickel coating . The HandiMag
can be used to separate the magnetic particles in various containers such as
microfuge tubes, test tubes or centrifuge tubes by holding the magnet
against the wall of containers by hand or with a rubber band.
(E) The SPHEROTM MiniTube Mag Separator (Cat. No.:
MTMS-16) holds up to sixteen 1.5 mL tubes.
(F) The SPHEROTM UltraMag Separator (Cat. No.: UMS-3000)
and SPHEROTM UltraMag DW Separator
(Cat. No.: UMDS-1000) are designed to facilitate the
washing of magnetic particles in the Magnetic Particles
Enzyme Immunoassay (MPEIA) using 96-well plates. The
magnetic pegs of UltraMag Separator fit between the wells underneath the
96-well plate. The microplate is read in an appropriate microtiter plate
reader by loading the microplate with the attached UltraMag Separator into
the reader. The magnets ensure that the magnetic particles stay out of light
beam passing through the bottom of the well and corresponding holes in the
UltraMag Separator.
A combination of magnetic particles as solid phase and
conventional enzyme immuno assay (EIA) microplate technology offers
significant advantages over the conventional EIA. Some of the advantages are
listed below:
• Magnetic particles offer larger surface area and
significantly faster reaction kinetics; thus, reducing the total time to
complete an assay.
• Microparticles are washed more efficiently leaving less
residual reactants, thus, lowering background signal and potentially
improving sensitivity.
• Coating of magnetic particle is easier, gives a more
uniform solid phase and helps to minimize lot-to-lot variation.
See the MAGNETIC SEPARATOR section of the
TECHNICAL INFORMATION Page of the Spherotech website to see photos of
several magnetic separators.
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