Flow Cytometry Multiplex Bead Assay Particles

FLOW CYTOMETRY MULTIPLEX BEAD ASSAY PARTICLES

The Blue PAKs (Particle Array Kits) are designed to simplify multiplex assay development using most flow cytometers. The PAKs consists of Fluorescent Blue Particles with different intensities in PE-Cy5 channel and minimal fluorescence in FITC and PE channels with 488 nm excitation. Either or both FITC or PE can be used for detection. Spherotech offers several sets of Particle Array Kits. Sets can be used independently or mixed together (3.0, 3.5, 4.0 and 5.0 µm sizes available). Functionalized, Streptavidin, Biotin and antibody coated PAKs are available.
 

The Yellow Fluorescent Particle Kits consist of 2.8 µm, 3.6 µm or 7.6 µm particles with multiple fluorescence intensities in FITC channels. These kits are used to develop multiplex assays using either PE or PE-Cy5 tracers for detection.

 

The Pink Fluorescent Particle Kits consist of either 2.8 µm and 3.6 µm with twelve different intensities in the PE channel. FITC conjugates can be used for detection. They are supplied as kits of either six odd numbered peaks or six even numbered peaks for the 2.8 µm and 3.6 µm particle kits. Carboxyl Pink Fluorescent are available for covalent coupling of proteins, antibodies, or antigens. Goat anti-Mouse IgG (Fc), Streptavidin, and Biotin coated Pink Fluorescent Particles Kits are also available for other applications.

 

The Pink Fluorescent Particle Array Kits (FPAK) are similar to the Pink Fluorescent Particle Kits except that the FPAK particles are made from a higher quality polystyrene particle. These particles are designed for flow cytometry application. The 3.1 µm kit has nine different intensities, while the 4.45 µm kit has ten different intensities. The FPAK particles can be used in conjuction with the Blue PAKs for advanced multiplex applications.
 

SPHERO^TM Flow Cytometry Multiplex Bead Assay Particles

PAK-3567-7K ; Blue Particle Array Kit - 7 peaks, 10⁸/mL, 7x1 mL, 3.5-3.9 µm

PAK-4067-8K ; Blue Particle Array Kit - 8 peaks, 10⁸/mL, 8x1 mL, 4.0-4.5 µm

PAK-5067-10K ; Blue Particle Array Kit - 10 peaks, 10⁸/mL, 10x1 mL, 5.0-5.9 µm

PAK-5067-5A ; Blue Particle Array Kit - Odd # peaks, 10⁸/mL, 5x1 mL, 5.0-5.9 µm

PAK-5067-5B ; Blue Particle Array Kit - Even # peaks, 10⁸/mL, 5x1 mL, 5.0-5.9 µm

PAK-7067-9K ; Blue Particle Array Kit - 9 peaks, 10⁷/mL, 9x1 mL, 7.0-7.9 µm

FA-2552-6K ; Fluorescent Yellow Particle Kit, Odd # peaks, 0.5% w/v, 6x1 mL, 2.5-2.9 µm

FB-2552-6K ; Fluorescent Yellow Particle Kit, Even # peaks, 0.5% w/v, 6x1 mL, 2.5-2.9 µm

FA-3558-6K ; Fluorescent Pink Particle Kit, Odd # peaks, 0.5% w/v, 6x1 mL, 3.5-3.9 µm

FB-3558-6K ; Fluorescent Pink Particle Kit, Even # peaks, 0.5% w/v, 6x1 mL, 3.5-3.9 µm

FPAK-3058-9K ; Pink Fluorescent Particle Array Kit - 9 peaks, 10⁸/mL, 9x1 mL, 3.0-3.4 µm

FPAK-4058-9K ; Pink Fluorescent Particle Array Kit - 10 peaks, 10⁸/mL, 10x1 mL, 4.0-4.5 µm

FX-3552-9K ; Fluorescent Yellow Particle Kit, 9 peaks, 0.5% w/v, 9x1 mL, 3.5-3.9 µm

SPHERO^TM Carboxyl Flow Cytometry Multiplex Bead Assay Particles

CPAK-3567-7K ; Carboxyl Blue Particle Array Kit - 7 peaks, 10⁸/mL, 7x1 mL, 3.5-3.9 µm

CPAK-4067-8K ; Carboxyl Blue Particle Array Kit - 8 peaks, 10⁸/mL, 8x1 mL, 4.0-4.5 µm

CPAK-5067-10K ; Carboxyl Blue Particle Array Kit - 10 peaks, 10⁸/mL, 10x1 mL, 5.0-5.9 µm

CPAK-5067-5A ; Carboxyl Blue Particle Array Kit - Odd # peaks, 10⁸/mL, 5x1 mL, 5.0-5.9 µm

CPAK-5067-5B ; Carboxyl Blue Particle Array Kit - Even # peaks, 10⁸/mL, 5x1 mL, 5.0-5.9 µm

CPAK-7067-9K ; Carboxyl Blue Particle Array Kit - 9 peaks, 10⁷/mL, 9x1 mL, 7.0-7.9 µm

CPAK-7052-3K ; Carboxyl Fluorescent Yellow Particle Array Kit - 3 peaks, 10⁷/mL, 3x1 mL, 7.0-7.9 µm

CPAK-7052-6K ; Carboxyl Fluorescent Yellow Particle Array Kit - 6 peaks, 10⁷/mL, 6x1 mL, 7.0-7.9 µm

CFP-5067-2 ; Carboxyl Blue Particle Array Chemistry Development Particles, 10⁸/mL, 2 mL, 5.0-5.9 µm

CFA-2552-6K ; Carboxyl Coated Fluorescent Yellow Particle Kit, Odd # peaks, 0.5% w/v, 6x1 mL, 2.5-2.9 µm

CFB-2552-6K ; Carboxyl Coated Fluorescent Yellow Particle Kit, Even # peaks, 0.5% w/v, 6x1 mL, 2.5-2.9 µm

CFA-2558-6K ; Carboxyl Coated Fluorescent Pink Particle Kit, Odd # peaks, 0.25% w/v, 6x1 mL, 2.5-2.9 µm

CFB-2558-6K ; Carboxyl Coated Fluorescent Pink Particle Kit, Even # peaks, 0.25% w/v, 6x1 mL, 2.5-2.9 µm

CFA-3558-6K ; Carboxyl Coated Fluorescent Pink Particle Kit, Odd # peaks, 0.25% w/v, 6x1 mL, 3.5-3.9 µm

CFB-3558-6K ; Carboxyl Coated Fluorescent Pink Particle Kit, Even # peaks, 0.25% w/v, 6x1 mL, 3.5-3.9 µm

CFX-3552-9K ; Carboxyl Fluorescent Yellow Particle Kit, 9 peaks, 0.5% w/v, 9x1 mL, 3.5-3.9 µm

SPHERO^TM Goat anti-Mouse Coated Flow Cytometry Multiplex Bead Assay Particles

MPAK-5067-10K ; Goat anti-Mouse IgG Blue Particle Array Kit - 10 peaks, 10⁶/mL, 10x1 mL, 5.0-5.9 µm

MPAK-5067-5A ; Goat anti-Mouse IgG Blue Particle Array Kit- Odd # peaks, 10⁶/mL, 5x1 mL, 5.0-5.9 µm

MPAK-5067-5B ; Goat anti-Mouse IgG Blue Particle Array Kit- Even # peaks, 10⁶/mL, 5x1 mL, 5.0-5.9 µm

MFA-3558-6K ; Goat anti-Mouse IgG Coated Fluorescent Pink Particle Kit, Odd # peaks, 0.1% w/v, 6x1 mL, 3.5-3.9 µm

MFB-3558-6K ; Goat anti-Mouse IgG Coated Fluorescent Pink Particle Kit, Even # peaks, 0.1% w/v, 6x1 mL, 3.5-3.9 µm

SPHERO^TM Streptavidin Coated Flow Cytometry Multiplex Bead Assay Particles

SVPAK-5067-10K ; Streptavidin Blue Particle Array Kit - 10 peaks, 10⁶/mL, 10x1 mL, 5.0-5.9 µm

SVPAK-5067-5A ; Streptavidin Blue Particle Array Kit - Odd # peaks, 10⁶/mL, 5x1 mL, 5.0-5.9 µm

SVPAK-5067-5B ; Streptavidin Blue Particle Array Kit - Even # peaks, 10⁶/mL, 5x1 mL, 5.0-5.9 µm

SVFA-2552-6K ; Streptavidin Coated Fluorescent Yellow Particle Kit, Odd # peaks, 0.1% w/v, 6x1 mL, 2.5-2.9 µm

SVFB-2552-6K ; Streptavidin Coated Fluorescent Yellow Particle Kit, Even # peaks, 0.1% w/v, 6x1 mL, 2.5-2.9 µm

SVFA-2558-6K ; Streptavidin Coated Fluorescent Pink Particle Kit, Odd # peaks, 0.1% w/v, 6x1 mL, 2.5-2.9 µm

SVFB-2558-6K ; Streptavidin Coated Fluorescent Pink Particle Kit, Even # peaks, 0.1% w/v, 6x1 mL, 2.5-2.9 µm

SPHERO^TM Biotin Coated Flow Cytometry Multiplex Bead Assay Particles

TFA-3558-6K ; Biotin Coated Fluorescent Pink Particle Kit, Odd # peaks, 0.1% w/v, 6x1 mL, 3.5-3.9 µm

TFB-3558-6K ; Biotin Coated Fluorescent Pink Particle Kit, Even # peaks, 0.1% w/v, 6x1 mL, 3.5-3.9 µm

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Last modified: feb-07