|
| |
Other Antigens Spec
| Item |
Rabbit anti-Alkaline Phosphatase |
|
| Host |
Rabbit |
| Catalog Number |
RALK-65 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% sodium azide |
| Storage |
4C |
| Procedure |
Rabbits were immunized with purified Alkaline
Phosphatase from calf intestine in Freund's adjuvant. |
| Specification |
By IEP this anti-alkaline phosphatase forms a single
precipitin band against purified alkaline phosphatase. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-Biotin |
|
| Host |
Goat |
| Catalog Number |
GBIO-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with biotinylated protein
carrier in Freunds adjuvant. The resuting antiserum was passed over an
antigen containing immunoabsorbant from which the anti-biotin was
immunoaffinity purified. |
| Specification |
Suitable for coupling to latex or magnetic particles.
|
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Goat anti-Biotin |
|
| Host |
Goat |
| Catalog Number |
GBIO-65F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with biotinylated protein
carrier in Freunds adjuvant. The resuting antiserum was passed over an
antigen containing immunoabsorbant from which the anti-biotin was
immunoaffinity purified. The resulting affinity purified goat
anti-biotin was conjugated with fluorescein isothiocyanate (FITC). |
| Specification |
This anti-biotin has a molar F/P ratio of 3-6. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Goat anti-Biotin |
|
| Host |
Goat |
| Catalog Number |
GBIO-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with biotinylated protein
carrier in Freunds adjuvant. The resuting antiserum was passed over an
antigen containing immunoabsorbant from which the anti-biotin was
immunoaffinity purified. The resulting affinity purified goat
anti-biotin was conjugated with horseradish peroxidase (HRP). |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:10,000-1:40,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Bird IgG |
|
| Host |
Rabbit |
| Catalog Number |
RBG-65A |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified bird IgG
isolated from several species of wild birds (crow included). The
resulting anti-IgG was solid phase absorbed to make it mu chain specific
and the anti-IgG was immunoaffinity purified off a bird IgG
immunoabsorbant |
| Specification |
The affinity purified antibodies react only with
bird IgG by immunodiffusion and IEP techniques. This product is suitable
for ELISA and Blotting applications. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Rabbit anti-Bird IgM |
|
| Host |
Rabbit |
| Catalog Number |
RBM-65A |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified bird IgM
isolated from several species of wild birds (crow, blackbird, sparrow
and starling included). The resulting anti-IgM was solid phase absorbed
to make it mu chain specific and the anti mu was immunoaffinity purified
off a bird IgM immunoabsorbant |
| Specification |
The affinity purified antibodies react only with
bird IgM by immunodiffusion and IEP techniques. This product is suitable
for ELISA and Blotting applications. |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
HRP Conjugated Rabbit anti-Bird IgM |
|
| Host |
Rabbit |
| Catalog Number |
RBM-65P |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified bird IgM
isolated from several species of wild birds (crow included). Serum was
collected and solid phase absorbed before being immunoaffinity purified
off an antigen containing immunoabsorbant. The resulting anti-IgM was
conjugated with horseradish peroxidase (HRP). |
| Specification |
The affinity purified antibodies react only with
bird IgM by immunodiffusion and IEP techniques. This product is suitable
for ELISA and Blotting applications. |
| Western Blots |
1:5,000-1:25,000 |
| ELISA |
1:12,000-1:50,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Rabbit anti-BrdU |
|
| Host |
Rabbit |
| Catalog Number |
RBDU-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with BrdU-KLH in Freunds
adjuvant. The resuting antiserum was passed over an antigen containing
immunoabsorbant from which the anti-BrdU was immunoaffinity purified. |
| Specification |
The anti-BrdU |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-BrdU |
|
| Host |
Rabbit |
| Catalog Number |
RBDU-65A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with BrdU-KLH in Freunds
adjuvant. The resuting rabbit anti-BrdU antiserum was passed over an
antigen containing immunoabsorbant from which the anti-BrdU was
immunoaffinity purified. |
| Specification |
anti-BrdU |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-Ferret IgG
h+l |
|
| Host |
Chicken |
| Catalog Number |
CFHL-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES, 0.1 M Sodium Chloride, 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with highly purified Ferret
IgG isolated from normal pooled ferret serum. Eggs were collected and an
IgY fraction was prepared. The anti-Ferret IgG was immunoaffinity
purified off a solid phase immunoabsorbant and conjugated with
horseradish peroxidase. |
| Specification |
The affinity purified anti-Ferret IgG reacts with
llama immunoglobulins and the papain derived Fc and Fab fragments by
ELISA, IEP and gel diffusion techniques. |
| Western Blots |
0.2 ug/ml |
| ELISA |
1:10,000 - 1:50,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Chicken anti-Ferret
IgG h+l |
|
| Host |
Goat |
| Catalog Number |
CFHL-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with Ferret IgG intact
molecule. After multiple immunizations eggs were collected and an IgY
fraction was prepared. The anti-Ferret IgG was then immunoaffinity
purified off an antigen containing immunoabsorbant. |
| Western Blots |
1:5,000 - 1:20,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-Ferret IgG
h+l |
|
| Host |
Goat |
| Catalog Number |
GFHL-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with Ferret IgG intact
molecule. After multiple immunizations in Freund's adjuvant the Goats
were bled, serum collected and the anti-Ferret IgG was immunoaffinity
purified off an antigen containing immunoabsorbant. |
| Western Blots |
1:5,000 - 1:20,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Goat anti-Ferret IgG h+l |
|
| Host |
Goat |
| Catalog Number |
GFHL-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Goats were immunized with Ferret IgG intact
molecule. After multiple immunizations in Freund's adjuvant the Goats
were bled and serum collected. The anti-Ferret IgG was affinity purified
and conjugated to horseradish peroxidase (HRP) |
| Specification |
Optimal conjugate working dilutions should be
determined for your particular assay conditions |
| Western Blots |
1 ug/ml - 0.2 ug/ml |
| ELISA |
0.2 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Gerbil
IgG h+l |
|
| Host |
Rabbit |
| Catalog Number |
RGBL-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with gerbil IgG intact
molecule. After multiple immunizations in Freund¡¯s adjuvant, the
rabbits were bled and the serum collected. The specific antibodies were
immunoaffinity purified off an IgG affinity column |
| Specification |
By immunoelectrophoresis and double diffusion, the
affinity purified antibodies form a precipitin arc against the IgG in
gerbil serum |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-Gerbil IgG
h+l |
|
| Host |
Rabbit |
| Catalog Number |
RGBL-65F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified gerbil
IgG isolated from normal gerbil serum. The resulting anti-IgG was
immunoaffinity purified off a gerbil IgG immunoabsorbant and conjugated
with fluorescein isothiocyanate (FITC) |
| Specification |
Molar F/P ratio = 4.5 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-Gerbil IgG
h+l |
|
| Host |
Rabbit |
| Catalog Number |
RGBL-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified gerbil IgG
isolated from pooled normal gerbil serum. The anti-IgG was
immunoaffinity purified off an IgG-containing solid phase
immunoabsorbant and conjugated with horseradish peroxidase (HRP) |
| Specification |
By immunoelectrophoresis and double diffusion, the
affinity purified antibodies form a precipitin arc against the IgG in
gerbil serum.In a direct ELISA against purified gerbil IgG, conjugate
diluted to 1/50,000 produced an O.D.=1.0 in a 15 minute reaction with
TMB as substrate. Optimal conjugate working dilutions should be
determined for your particular assay conditions |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-Hamster IgG
h+l |
|
| Host |
Goat |
| Catalog Number |
GHHL-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with highly purified hamster
IgG isolated from normal pooled hamster serum. Antiserum was collected
and the anti-Hamster IgG was then affinity purified off an antigen
containing immunoabsorbent. |
| Specification |
The anti-hamster IgG reacts with IgG and the Fab and
Fc fragments of IgG by immunodiffusion and IEP techniques. It also
reacts with both Kappa and Lambda light chains. |
| Western Blots |
1 ug/ml |
| ELISA |
capture 1:200 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Goat anti-Hamster IgG
h+l absorbed vs. Mouse and Rat |
|
| Host |
Goat |
| Catalog Number |
GHHL-65XA |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with highly purified hamster
IgG isolated from normal pooled hamster serum. Antiserum was collected
and solid phase absorbed vs. normal mouse and rat IgG. The anti-Hamster
IgG was then affinity purified off a hamster IgG containing
immunoabsorbent. |
| Specification |
The anti-hamster IgG reacts with IgG and the Fab and
Fc fragments of IgG by immunodiffusion and IEP techniques. It also
reacts with both Kappa and Lambda light chains. There is minimal
reactivity with mouse and rat IgG. |
| Western Blots |
1 ug/ml |
| ELISA |
capture 1:200 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Rabbit anti-KLH |
|
| Host |
Rabbit |
| Catalog Number |
RKLH-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified KLH. The
resulting anti KLH was immunoaffinity purified off a KLH immunoabsorbant. |
| Specification |
The affinity purified antibodies react with KLH by
immunodiffusion and IEP techniques |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-Llama IgG
h+l |
|
| Host |
Chicken |
| Catalog Number |
CLHL-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES, 0.1 M Sodium Chloride, 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with highly purified Llama
IgG isolated from normal pooled llama serum. Eggs were collected and an
IgY fraction was prepared. The anti-Llama IgG was immunoaffinity
purified off a solid phase immunoabsorbant and conjugated with
horseradish peroxidase. |
| Specification |
The affinity purified anti-Llama IgG reacts with
llama immunoglobulins and the papain derived Fc and Fab fragments by
ELISA, IEP and gel diffusion techniques. |
| Western Blots |
0.2 ug/ml |
| ELISA |
1:10,000 - 1:50,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Chicken anti-Llama
IgG h+l |
|
| Host |
Goat |
| Catalog Number |
CLGH-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with Llama IgG intact
molecule. After multiple immunizations eggs were collected and an IgY
fraction was prepared. The anti-Llama IgG was then immunoaffinity
purified off an antigen containing immunoabsorbant. |
| Western Blots |
1:5,000 - 1:20,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-Llama IgG
h+l |
|
| Host |
Goat |
| Catalog Number |
GLGH-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with Llama IgG intact molecule.
After multiple immunizations in Freund's adjuvant the Goats were bled,
serum collected and the anti-Llama IgG was immunoaffinity purified off
an antigen containing immunoabsorbant. |
| Western Blots |
1:5,000 - 1:20,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Goat anti-Llama IgG h+l |
|
| Host |
Goat |
| Catalog Number |
GLGH-65 |
| Quantity |
2.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with Llama IgG intact molecule.
After multiple immunizations the Goats were bled, serum was collected
and absorbed to ensure anti-Llama IgG h+l specificity. |
| Specification |
The anti-llama IgG is suitable for
immunoelectrophoresis and RID applications. |
| Western Blots |
1:5,000 - 1:20,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-Monkey IgG
h+l |
|
| Host |
Goat |
| Catalog Number |
GRHG-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with Monkey IgG intact
molecule. After multiple immunizations, the Goats were bled and serum
collected. The anti-Monkey IgG was then immunoaffinity purified off an
antigen containing immunoabsorbant. |
| Western Blots |
1:1000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Goat anti-Monkey IgG h+l |
|
| Host |
Goat |
| Catalog Number |
GRHG-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Goats were immunized with Monkey IgG intact
molecule. After multiple immunizations the goats were bled and serum
collected. The anti-Monkey IgG was immunoaffinity purified and
conjugated to horseradish peroxidase (HRP) |
| Specification |
Optimal anti-Monkey IgG working dilutions should be
determined for your particular assay conditions |
| Western Blots |
1 ug/ml - 0.2 ug/ml |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Monkey
IgG h+l |
|
| Host |
Rabbit |
| Catalog Number |
RRHG-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with Monkey IgG intact
molecule. After multiple immunizations in Freund's adjuvant the Rabbits
were bled and serum collected. The antibodies were isolated off an
antigen containing immunoabsorbant. |
| Western Blots |
1,2000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-Monkey IgG
h+l |
|
| Host |
Rabbit |
| Catalog Number |
RRHG-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with Monkey IgG intact
molecule. After multiple immunizations in Freund's adjuvant the Goats
were bled and serum collected. The anti-Monkey IgG was immunoaffinity
purified and conjugated with horseradish peroxidase (HRP) |
| Specification |
Optimal anti-Monkey IgG working dilutions should be
determined for your particular assay conditions |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
0.2 ug/ml - 0.05 ug/ |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-Protein
A |
|
| Host |
Chicken |
| Catalog Number |
CPA-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein A. After multiple immunizations in Freund’s adjuvant, eggs were
collected and delipidated. Anti-Protein A antibodies were immunoaffinity
purified off protein A agarose. |
| Specification |
Recommended working dilution for detection antibody
in ELISA assay for this anti-protein A is 1:10,000-1:50,000. |
| Western Blots |
1:1,000 – 1:10,000 |
| ELISA |
Capture= 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Chicken
anti-Protein A |
|
| Host |
Chicken |
| Catalog Number |
CPA-165D-Z |
| Quantity |
2.0 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with recombinant Protein A
in Freund's adjuvant. After multiple immunizations, eggs were collected
from the chickens, the IgY fraction prepared and the anti-Protein A was
isolated by immunoaffinity chromatography. The resulting chicken
anti-Protein A was then coupled to an agarose gel. |
| Specification |
The anti-Protein A shows a single precipitin arc
against recombinant protein A. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-Protein
A |
|
| Host |
Chicken |
| Catalog Number |
CPA-65B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein A. After multiple immunizations in Freund’s adjuvant, eggs were
collected and delipidated. Antibodies were immunoaffinity purified off
protein A agarose and conjugated with biotin. |
| Western Blots |
1:1,000 – 1:5,000 |
| ELISA |
1:10,000-1:50,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-Protein A |
|
| Host |
Chicken |
| Catalog Number |
CPA-65F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 with 0.2% BSA. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein A. After multiple immunizations in Freund’s adjuvant, eggs were
collected and delipidated. Antibodies were immunoaffinity purified off
protein A agarose and conjugated with fluorescein isothiocyanate (FITC). |
| Western Blots |
1:1,000 – 1:5,000 |
| ELISA |
detection 1:5,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-Protein A |
|
| Host |
Chicken |
| Catalog Number |
CPA-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein A. After multiple immunizations in Freund’s adjuvant, eggs were
collected and delipidated. Antibodies were immunoaffinity purified off
protein A agarose and conjugated with horseradish peroxidase (HRP). |
| Western Blots |
1:1,000 – 1:10,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-Protein
G |
|
| Host |
Chicken |
| Catalog Number |
CPG-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein G lacking the albumin binding region. After multiple
immunizations in Freund’s adjuvant, eggs were collected and delipidated.
Antibodies were immunoaffinity purified off protein G agarose. |
| Western Blots |
1:5,000 – 1:20,000 |
| ELISA |
Capture= 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-Protein
G |
|
| Host |
Chicken |
| Catalog Number |
CPG-65B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein G lacking the albumin binding region. After multiple
immunizations in Freund’s adjuvant, eggs were collected and delipidated.
Antibodies were immunoaffinity purified off protein G agarose and
conjugated with biotin. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:10,000 - 1:50,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-Protein G |
|
| Host |
Chicken |
| Catalog Number |
CPG-65F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 with 0.2% BSA. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein G lacking the albumin binding region. After multiple
immunizations in Freund’s adjuvant, eggs were collected and delipidated.
Antibodies were immunoaffinity purified off protein G agarose and
conjugated with fluorescein isothiocyanate (FITC). |
| Specification |
Fluoresceine/Protein ratio = 3.2 |
| Western Blots |
1:2000 |
| ELISA |
1:2000 - 1:10,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-Protein G |
|
| Host |
Chicken |
| Catalog Number |
CPG-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1M sodium chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
protein G lacking the albumin binding region. After multiple
immunizations in Freund’s adjuvant, eggs were collected and delipidated.
Antibodies were immunoaffinity purified off protein G agarose and
conjugated with horseradish peroxidase (HRP). |
| Western Blots |
1:1,000 – 1:10,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-SP2/0 |
|
| Host |
Rabbit |
| Catalog Number |
RSP2-65A |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized multiple times with whole
SP2/0 cells followed with lysed SP2/0 cells. After multiple
immunizations in Freunds adjuvant, serum was collected. Antibodies were
solid phase absorbed with human IgG then immunoaffinity purified using a
SP2/0 column. Antibody concentration was determined by extinction
coefficient : O.D. 1.4 at 280nM equals 1.0 mg |
| Specification |
ELISA: Antibody specificity was verified by ELISA
against wells sensitized with containing 10ug/ml of antigen. A 1:5,000
dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using
ICL, Inc HRPO conjugated Donkey anti-rabbit (IgG) at 1:15,000 and TMB as
the substrate. Appropriate specificity controls were run. |
| Western Blots |
1:500-1:1000 |
| ELISA |
coating: 1:200 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
|
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