|
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Infectious Disease Reagents Spec
| Item |
HRP Conjugated Human anti-Dengue (IgG
Fraction) |
|
| Host |
Human |
| Catalog Number |
HDV-55GP |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Serum was obtained from a patient who has had
multiple infections with dengue viruses. This patient had an HAI anti
dengue titer of >15,000. An IgG fraction was prepared using ion exchange
chromatography and the IgG conjugated with horseradish peroxidase (HRP). |
| Specification |
The HRP conjugated IgG fraction reacts with each of
the dengue serotypes when tested by ELISA at 1:5000 dilution . It also
reacts in dot blot and can be used in Western Blotting at 1:1000 or
greater dilution. On Westerns, it reacts with both preM and E proteins.
The required dilutions must be determined for the particular assay
employed. |
| Western Blots |
1:1,000 |
| ELISA |
1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Human IgG anti-Dengue |
|
| Host |
Human |
| Catalog Number |
HGDV-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% sodium azide |
| Storage |
4C for short term: Frozen for long term |
| Procedure |
PLASMA TESTED AND FOUND NEGATIVE FOR HIV AND
HEPATITIS B AND C.AS WITH ALL HUMAN MATERIALS, MUST BE HANDLED AS IF
CAPABLE OF TRANSMITTING INFECTIOUS DISEASE |
| Specification |
Lot has been tested and found negative for HIV ? and
Hepatitis B and C, using standard laboratory procedures. Has an HAI
titer of greater than 1:1250 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
FOR USE IN RESEARCH AND MANUFACTURING ONLY; NOT FOR
USE IN DIAGNOSTIC APPLICATIONS |
| Item |
Human IgG anti-Dengue (IgG Fraction) |
|
| Host |
Human |
| Catalog Number |
HDV-55G |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
TBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Serum was obtained from a patient who has had
multiple infections with dengue viruses. This patient had an HAI anti
dengue titer of >15,000. An IgG fraction was prepared using ion exchange
chromatography. |
| Specification |
The IgG fraction reacts with each of the dengue
serotypes when tested by ELISA at 1:5000 dilution . It also reacts in
dot blot and can be used in Western Blotting at 1:1000 or greater
dilution. On Westerns, it reacts with both preM and E proteins. The
required dilutions must be determined for the particular assay employed. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Human IgM anti-Dengue |
|
| Host |
Human |
| Catalog Number |
HMDV-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
N/A |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Obtained from patients with confirmed dengue. This
Lot has been tested by standard laboratory procedures and found to be
negative for HIV 1 and 2 and Hepatitis B and C. As with all human
tissues and fluids, treat as potentially capable of transmitting
infectious agents. |
| Specification |
Titers were determined by IgM-capture ELISA |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Dengue (Pan) |
|
| Host |
Mouse |
| Catalog Number |
MDVP-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C for short term: Do not multiply freeze thaw |
| Procedure |
Mice were immunized with a mixture of dengue 1, 2, 3
and 4. Spleen cells were used to prepare hybridomas and clones selected
based on the ability of the antibody they produced to react with each
dengue serotype |
| Specification |
This anti-Dengue was produced from tissue culture
and purified using Protein A- affinity chromatography. The myeloma cell
line used as a fusion partner produces small amounts of IgG1, therefore
IgG1 is sometimes present in the preparation.IgG 2A Kappa This hybridoma
antibody reacts with each dengue serotype in dot blot, ELISA and
fluorescent antibody tests. Its reactivity has been confirmed by CDC.
Please note that the reactivity of this antibody varies between strains
and various isolates, therefore, a single dilution may not be
appropriate for each situation |
| Western Blots |
1 ug/ml |
| Item |
Monoclonal anti-Dengue Type 1 |
|
| Host |
Mouse |
| Catalog Number |
MDV1-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Mice were immunized with purified dengue serotype 1.
Spleen cells were used to prepare hybridomas and clones selected based
on their ability to react only with dengue 1. |
| Specification |
This anti-Dengue 1 is produced from tissue culture
and purified using Protein A-affinity chromatography. This anti-dengue
1(clone ICL1) is an IgG1 kappa which reacts only with dengue 1 and NOT
with dengue 2, 3 or 4 by ELISA and F.A techniques. |
| Western Blots |
1ug/ml |
| Item |
Monoclonal anti-Dengue Type 2 |
|
| Host |
Mouse |
| Catalog Number |
MDV2-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Mice were immunized with purified dengue serotype 2.
Spleen cells were used to prepare hybridomas and clones selected based
on their ability to react only with dengue 2 |
| Specification |
All hybridoma proteins are produced from tissue
culture and purified using Protein A-affinity chromatography. Two clones
are available: Clone 5 is an IgG1 kappa Clone 9 is an IgG 2A kappa These
antibodies react only with dengue 2 and NOT with dengue 1, 3 or 4 by
ELISA and F.A techniques |
| Item |
Monoclonal anti-Dengue Type 3 |
|
| Host |
Mouse |
| Catalog Number |
MDV3-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Mice were immunized with purified dengue serotype 3.
Spleen cells were used to prepare hybridomas and clones selected based
on their ability to react only with dengue 3. |
| Specification |
All hybridoma proteins are produced from tissue
culture and purified using Protein A-affinity chromatography. This clone
(ICL3) is an IgG1 kappa which reacts only with dengue 3 and NOT with
dengue 1, 2 or 4 by ELISA and F.A techniques. |
| Western Blots |
1ug/ml |
| Item |
Monoclonal anti-Dengue Type 4 |
|
| Host |
Mouse |
| Catalog Number |
MDV4-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Mice were immunized with purified dengue serotype 4.
Spleen cells were used to prepare hybridomas and clones selected based
on their ability to react only with dengue 4. |
| Specification |
All hybridoma proteins are produced from tissue
culture and purified using Protein A-affinity chromatography. This clone
(ICL1) is an IgG1 kappa which reacts only with dengue 4 and NOT with
dengue 1, 2 or 3 by ELISA and F.A techniques. |
| Western Blots |
1ug/ml |
| Item |
Rabbit anti-Dengue (IgG Fraction) |
|
| Host |
Rabbit |
| Catalog Number |
RDV-55G |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
Frozen for long term or 4 C for short term |
| Procedure |
Rabbits were immunized with a mixture of inactivated
dengue viruses of Types 1, 2, 3, and 4. Antigen was given in Freunds
adjuvant. The resulting antiserum was absorbed on a fetal calf serum
affinity column. An IgG fraction was prepared using Protein G affinity
chromatography. |
| Specification |
The antiserum reacts with each of the dengue
serotypes when tested by fluorescent antibody techniques using virus
infected cells. It also reacts in dot blot and ELISA procedures. The
required dilutions must be determined for the particular assay employed.
The antigens were grown in mosquito cell lines containing fetal calf
serum therefore minor reactivity might be expected to antigens from
these sources. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Rabbit anti-E-coli Heat Labile Toxin |
|
| Host |
Rabbit |
| Catalog Number |
RECO-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
RABBITS WERE IMMUNIZED WITH THE HIGHLY PURIFIED BETA
SUBUNIT OF E COLI HLT, PREPARED USING RECOMBINANT PROTEIN METHODS, IN
FREUNDS ADJUVANT. |
| Specification |
THE METHOD USED TO EVALUATE THE ANTISERUM IS BASED
UPON A GM1 RECEPTOR ELISA ASSAY. THE TITER IS GREATER THAN 1:20,000 WITH
THE BETA SUBUNIT OF HLT AND ABOUT 1:6000 WITH THE BETA SUBUNIT OF
V.CHOLERA TOXIN |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Rabbit anti-E-coli Heat Labile Toxin (IgG
Fraction) |
|
| Host |
Rabbit |
| Catalog Number |
RECO-55G |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
TBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Publications |
Kang, T. et al., App. Biochem&Biotech June 2004,
Volume 117, Issue 3, pps. 175-188 |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with the highly purified Beta
subunit of E. coli HLT, prepared using recombinant protein methods, in
Freund's adjuvant. |
| Specification |
The method used to evaluate the IgG antibody is
based upon a GM1 receptor ELISA assay. The titer is greater than 1:15000
with the Beta subunit of HLT and about 1:4000 with the Beta subunit of
V. cholera toxin. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Chicken
anti-Hepatitis B |
|
| Host |
Chicken |
| Catalog Number |
CHPB-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
0.01M TRIS-0.14M SODIUM CHLORIDE pH 7.2 |
| Preservative |
0.1% SODIUM AZIDE |
| Storage |
4 C |
| Procedure |
CHICKENS WERE IMMUNIZED WITH HIGHLY PURIFIED Ad/Ay
ANTIGEN. EGGS WERE COLLECTED AND THE IGY FRACTION PREPARED. THE ANTI Ad/Ay
WAS IMMUNOAFFINITY PURIFIED FROM THE IGY FRACTION |
| Specification |
THIS ANTIBODY IS SUITABLE FOR ELISA, PRECIPITATION,
FLUORESCENCE AND IMMUNOHISTOCHEMISTRY TECHNIQUES. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Affinity Purified Goat anti-Hepatitis B |
|
| Host |
Goat |
| Catalog Number |
GHPB-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
0.01M TRIS-0.14M SODIUM CHLORIDE pH 7.2 |
| Preservative |
0.1% SODIUM AZIDE |
| Storage |
4 C |
| Procedure |
GOATS WERE IMMUNIZED WITH HIGHLY PURIFIED HEPATITIS
B ANTIGEN. SERUM WAS COLLECTED AND THE ANTI Ad/Ay WAS IMMUNOAFFINITY
PURIFIED |
| Specification |
THIS ANTIBODY IS SUITABLE FOR ELISA, PRECIPITATION,
FLUORESCENCE AND IMMUNOHISTOCHEMISTRY TECHNIQUES. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Affinity Purified Horse anti-Hepatitis
B |
|
| Host |
HORSE |
| Catalog Number |
HHPB-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
0.01M PHOSPHATE-0.15M SODIUM CHLORIDE pH 7.2 |
| Preservative |
0.1% SODIUM AZIDE |
| Storage |
4 C |
| Procedure |
HORSES WERE IMMUNIZED WITH HIGHLY PURIFIED Ad/Ay
ANTIGEN. SERUM WAS COLLECTED AND THE ANTI BODY IMMUNOAFFINITY PURIFIED
FROM NON-RECOMBINANT HEPATITIS ANTIGENS |
| Specification |
THIS ANTIBODY IS SUITABLE FOR ELISA, PRECIPITATION,
FLUORESCENCE AND IMMUNOHISTOCHEMISTRY TECHNIQUES. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Affinity Purified Chicken
anti-Plasmodium Aldolase |
|
| Host |
Chicken |
| Catalog Number |
CPVA-55A |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified recombinant
plasmodium aldolase. Eggs were collected and an IgY fraction was
prepared before the anti-aldolase was affinity purified off an antigen
containing immunoabsorbant. |
| Specification |
The anti-aldolase is suitable for ELISA capture
antibody as well as for lateral flow assays. |
| Western Blots |
1.0 ug/ml |
| ELISA |
0.5 ug/ml |
| Application |
Useful for distinguishing between Pl. Falciparum and
Pl. Vivax when used in combination with ICL's monoclonal anti-Pl
Falciparum. For Research and Manufacturing Use Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal IgG anti-Plasmodium Aldolase |
|
| Host |
Mouse |
| Catalog Number |
MPVA-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% Sodium Azide |
| Storage |
4 C for short term; -20 C or lower for long term but
DO NOT multiply freeze-thaw |
| Procedure |
Immunization of mice with the plasmodium aldolase
followed by hybridoma production and subsequent purification of the IgG
antibody from ascitic fluid using proprietary techniques |
| Specification |
IgG1 Kappa Greater than 95% IgG by gel
electrophoresis Used successfully in ELISA, Western Blots, Dot Blots and
Lateral Flow assays. |
| Western Blots |
1ug/ml - 0.2 ug/ml |
| ELISA |
capture: 2 ug/ml |
| Item |
Monoclonal IgG anti-Plasmodium
falciparum |
|
| Host |
Mouse |
| Catalog Number |
MPFG-55A |
| Quantity |
1.0 mg |
| Physical State |
LIQUID |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% Sodium Azide |
| Storage |
4 C for short term; -20 C or lower for long term but
DO NOT multiply freeze-thaw |
| Procedure |
Immunization of mice with the histadine rich protein
II from Plasmodium falciparum followed by hybridoma production and
subsequent purification of the IgG antibody from ascitic fluid using
proprietary techniques |
| Specification |
IgG1 Kappa Greater than 95% IgG by gel
electrophoresis Used successfully in ELISA, Western Blots, Dot Blots and
Lateral Flow assays.Pairs with Monoclonal MPFM-45A in systems requiring
matched pairs.Recommended starting dilution: 1.0mg/1.0ml |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Monoclonal IgG anti-Plasmodium
falciparum Peroxidase Conjugated |
|
| Host |
Mouse |
| Catalog Number |
MPFG-55P |
| Quantity |
0.5 mg |
| Physical State |
Liquid |
| Preservative |
0.01% thimerosal |
| Storage |
4 C for short term |
| Procedure |
Immunization of mice with the histadine rich protein
II from Plasmodium falciparum followed by hybridoma production and
subsequent purification of the IgG antibody from ascitic fluid using
proprietary techniques. The purified antibody was conjugated with
horseradish peroxidase. |
| Western Blots |
start at 1 ug/ml |
| ELISA |
start at 0.5 ug/ml |
| Item |
Monoclonal IgM anti-Plasmodium
falciparum |
|
| Host |
Mouse |
| Catalog Number |
MPFM-55A |
| Quantity |
1.0 mg |
| Physical State |
LIQUID |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% Sodium Azide |
| Storage |
4 C for short term; -20 C or lower for long term but
DO NOT multiply freeze-thaw |
| Procedure |
Immunization of mice with the histadine rich protein
II from Plasmodium falciparum followed by hybridoma production and
subsequent purification of the IgM antibody from ascitic fluid using
proprietary techniques. |
| Specification |
IgM KappaGreater than 95% IgG by gel
electrophoresisUsed successfully in ELISA, Western Blots, Dot Blots and
Lateral Flow assays.Pairs with Monoclonal MPFG-45A in systems requiring
matched pairs. Recommended starting dilution: 1.0mg / 1.0ml |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Affinity Purified Rabbit
anti-Plasmodium Aldolase |
|
| Host |
Rabbit |
| Catalog Number |
RPVA-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified recombinant
plasmodium aldolase. The resulting antiserum was solid phase absorbed
before the anti-aldolase was affinity purified off an aldolase
containing immunoabsorbant. |
| Specification |
The anti-aldolase is suitable for ELISA capture
antibody as well as for lateral flow assays. |
| Western Blots |
1.0 ug/ml |
| ELISA |
0.5 ug/ml |
| Application |
Useful for distinguishing between Pl. Falciparum and
Pl. Vivax when used in combination with ICL's monoclonal anti-Pl
Falciparum. For Research and Manufacturing Use Only; Not for Use in
Diagnostic Applications. |
| Item |
HRP Conjugated Rabbit anti-Plasmodium
Aldolase |
|
| Host |
Rabbit |
| Catalog Number |
RPVA-55P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified recombinant
plasmodium aldolase. The resulting antiserum was solid phase absorbed
before the anti-aldolase was affinity purified off an aldolase
containing immunoabsorbant. The anti-aldolase was then conjugated with
horseradish peroxidase. |
| Specification |
The anti-aldolase is suitable for ELISA when paired
with: RPVA-55A and CPVA-55A |
| Western Blots |
1.0 ug/ml |
| ELISA |
0.5 ug/ml |
| Application |
Useful for distinguishing between Pl. Falciparum and
Pl. Vivax when used in combination with ICL's monoclonal anti-Pl
Falciparum. For Research and Manufacturing Use Only; Not for Use in
Diagnostic Applications. |
| Item |
Rabbit anti-Plasmodium Vivax (IgG
Fraction) |
|
| Host |
Rabbit |
| Catalog Number |
RVX-55G |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4C |
| Procedure |
PARASITES FROM THE BLOOD OF PATIENTS WITH CONFIRMED
INFECTION WITH PL. VIVAX WERE ISOLATED, WASHED AND USED TO IMMUNIZE
RABBITS. SERUM WAS COLLECTED AND AN IgG ENRICHED FRACTION WAS PREPARED
USING ION EXCHANGE CHROMATOGRAPHY |
| Specification |
The antiserum reacts with the Pl. vivax organism by
fluorescent antibody procedures and with the disrupted organism using
ELISA and dot blot procedures. No cross-reactivity studies have been
performed at this time. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat
anti-Streptococcus Pyogenes (Group A) |
|
| Host |
Goat |
| Catalog Number |
GSAC-55A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were intensely immunized, intravenously, with
killed Streptococcus pyogenes vaccine Strain J17A4. Serum was collected
and antibody to the Group A carbohydrate was immunoaffinity purified |
| Specification |
This antibody is suitable for ELISA, immunoblotting,
precipitation reactions, fluorescent antibody, immunohistochemistry,
conjugation with colloidal gold. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Horse anti-Clostridium botulinum Toxin
B |
|
| Host |
HORSE |
| Catalog Number |
HBOT-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
N/A |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Horses were immunized with small doses of Cl.
botulinum B in a proprietary adjuvant. |
| Specification |
The antiserum has a toxin neutralization titer of
100-200iu/ml |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Horse anti-Clostridium difficile Toxins
A+B |
|
| Host |
HORSE |
| Catalog Number |
HDIF-55 |
| Quantity |
1.0ml |
| Physical State |
Liquid |
| Buffer |
N/A |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Horses were immunized with small doses of Cl.
difficile toxins A+B in a proprietary adjuvant. |
| Specification |
Antisera function in toxin neutralization tests
using mice as the test animal |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing |
| Item |
Human anti-Chagas |
|
| Host |
Human |
| Catalog Number |
HTCZ-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% sodium azide |
| Storage |
4C for short term: Frozen for long term |
| Procedure |
PLASMA TESTED AND FOUND NEGATIVE FOR HIV AND
HEPATITIS B AND C.AS WITH ALL HUMAN MATERIALS, MUST BE HANDLED AS IF
CAPABLE OF TRANSMITTING INFECTIOUS DISEASE |
| Specification |
Serum/plasma are analyzed by an ELISA test developed
by Wiener Lab. This pool has on OD value of 2.02.All samples are tested
and found to be negative for HIV and Hepatitis B and C. As with all
human specimens, THESE MATERIALS MUST BE HANDLED AS IF CAPABLE OF
TRANSMITTING INFECTIOUS AGENTS.COUNTRY OF ORIGIN: HONDURAS |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
FOR USE IN RESEARCH AND MANUFACTURING ONLY; NOT FOR
USE IN DIAGNOSTIC APPLICATIONS |
| Item |
Human IgM anti-Leptospira |
|
| Host |
Human |
| Catalog Number |
HLPT-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% sodium azide |
| Storage |
4C for short term: Frozen for long term |
| Procedure |
PLASMA TESTED AND FOUND NEGATIVE FOR HIV AND
HEPATITIS B AND C.AS WITH ALL HUMAN MATERIALS, MUST BE HANDLED AS IF
CAPABLE OF TRANSMITTING INFECTIOUS DISEASE |
| Specification |
OBTAINED FROM A PATIENT DIAGNOSED WITH LEPTO.THE
PLASMA GAVE 3 DOTS ON THE PANBIO DIPSTICK. PLASMA TESTED AND FOUND
NEGATIVE FOR HIV AND HEPATITIS B AND C.AS WITH ALL HUMAN MATERIALS, MUST
BE HANDLED AS IF CAPABLE OF TRANSMITTING INFECTIOUS DISEASE |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
FOR USE IN RESEARCH AND MANUFACTURING ONLY; NOT FOR
USE IN DIAGNOSTIC APPLICATIONS |
| Item |
Rabbit anti-Hanta Virus |
|
| Host |
Rabbit |
| Catalog Number |
RSNV-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
A RECOMBINANT N-PROTEIN ANTIGEN FROM THE SIN NOMBRE
VIRUS WAS PREPARED AND USED TO IMMUNIZE RABBITS. |
| Specification |
The antiserum reacts with the N-protein of SNV by
ELISA and Western Blot analysis |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Rabbit anti-Vibrio Cholera Toxin |
|
| Host |
Rabbit |
| Catalog Number |
RCHL-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
RABBITS WERE IMMUNIZED WITH HIGHLY PURIFIED NATIVE
SUBUNIT OF CHOLERA TOXIN (CHOLERAGENOID) IN FREUNDS ADJUVANT |
| Specification |
THE METHOD USED TO EVALUATE THE ANTISERUM IS BASED
UPON A GM1 RECEPTOR ELISA ASSAY. THE TITER IS GREATER THAN 1:20,000 WITH
THE BETA SUBUNIT OF CHOLERA TOXIN AND ABOUT 1:6000 WITH THE BETA SUBUNIT
OF E. COLI HLT. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-West Nile |
|
| Host |
Mouse |
| Catalog Number |
MWNV-55-3B4 |
| Quantity |
1.0 ml |
| Physical State |
Lyophilized |
| Buffer |
Reconstitute with 1 ml distilled water |
| Preservative |
1 mM Phenylmethylsulphonyl Fluoride |
| Storage |
4 C |
| Procedure |
Prepared by hybridoma technology using Kunjin virus
Strain MRM16 as immunogen for Balb/c mice and NS1 cells as fusion
partners. Clones were selected for reactivity to West Nile Virus. The
clone number is 3B4. |
| Specification |
In an indirect fixed cell ELISA, product may be used
at about a 1:1000 dilution. It also reacts with Kunjin Virus. By Western
blot analysis this product reacts with the E protein. Clone 3B4 is an
IgG2A |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-West Nile |
|
| Host |
Mouse |
| Catalog Number |
MWNV-55-1D4 |
| Quantity |
1.0 ml |
| Physical State |
Lyophilized |
| Buffer |
Reconstitute with 1 ml distilled water |
| Preservative |
1 mM Phenylmethylsulphonyl Fluoride |
| Storage |
4 C |
| Procedure |
Prepared by hybridoma technology using Kunjin virus
Strain MRM16 as immunogen for Balb/c mice and NS1 cells as fusion
partners. Clones were selected for reactivity to West Nile Virus. The
clone number is 1D4. |
| Specification |
In an indirect fixed cell ELISA, product may be used
at about a 1:1000 dilution. It also reacts with Kunjin Virus. By Western
blot analysis, this product reacts with the E protein. Clone 1D4 is an
IgG1 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-West Nile |
|
| Host |
Mouse |
| Catalog Number |
MWNV-55-2B2 |
| Quantity |
1.0 ml |
| Physical State |
Solid |
| Buffer |
Reconstitute with 1 ml distilled water |
| Preservative |
1 mM Phenylmethylsulphonyl Fluoride |
| Storage |
4-8C for short term, frozen for long term storage |
| Procedure |
Prepared by hybridoma technology using Kunjin virus
Strain MRM16 as immunogen for Balb/c mice and NS1 cells as fusion
partners. Clone were selected for reactivity to West Nile Virus. The
clone number is 2B2. |
| Specification |
In an indirect fixed cell ELISA, product may be used
at about a 1:1000 dilution. It also reacts with Kunjin Virus. By Western
blot analysis, all react with the E protein. Only 2B2 exhibits
neutralizing activity of infected PS-EK cells. Clone 2B2 is an IgG2B
Clone 3B4 is an IgG2A Clone 1D4 is an IgG1 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Item |
Chagas Antigen |
|
| Host |
No Host |
| Catalog Number |
ATCZ-55 |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4C |
| Procedure |
Promastigotes grown in LIT medium at 25C and
harvested by centrifugation, washed with PBS, then disrupted by
sonication. Sonicate and/or vortex vigorously before use |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For ELISA, sensitize Immulon 2 plates with
approximately 10ul of antigen per well. For Western blot, antigen can be
diluted in a loading buffer of 50mM Tris-HCl, 100mM mercaptoethanol, 2%
SDS, 0.1% Bromophenol blue, and 10% glycerol |
| Item |
Dengue Viral Antigen Type 1 |
|
| Host |
No Host |
| Catalog Number |
ADV1-55 |
| Quantity |
Liquid |
| Physical State |
Tris-Borate-Saline pH 9 |
| Preservative |
4 degrees for short term: -70 for long term. Do not
repeatedly freeze-thaw |
| Storage |
Dengue 1,2,3, and 4 viruses were grown in C6/36
Ae.albopictus cells. Virus was concentrated by 7% PEG precipitation,
inactivated with Betapropiolactone for 48 hours and resuspended in
Tris-borate-saline. Inactivation is confirmed by culture and FA
techniques. |
| Procedure |
Contains about 32 HA units of antigen. Can be used
in direct and IgM capture ELISA. Bring to room temperature, vortex
vigorously, DO NOT centrifuge. Treat As If Containing Infectious Agents.
Antigen should be titrated to determine optimal concentrations for a
particular application but suggest 2-10 ug/well for direct ELISA |
| Specification |
3.1 mg/ml protein by Bradford Method |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
999999 |
| Item |
Dengue Viral Antigen Type 2 |
|
| Host |
No Host |
| Catalog Number |
ADV2-55 |
| Quantity |
Liquid |
| Physical State |
Tris-Borate-Saline pH 9 |
| Preservative |
4 degrees for short term: -70 for long term. Do not
repeatedly freeze-thaw |
| Storage |
Dengue 2 viruses were grown in C6/36 Ae.albopictus
cells. Virus was concentrated by 7% PEG precipitation, inactivated with
Betapropiolactone for 48 hours and resuspended in Tris-borate-saline.
Inactivation is confirmed by culture and FA techniques |
| Procedure |
Contains about 32 HA units of antigen. Can be used
in direct and IgM capture ELISA as well as lateral flow assays but a
dispersing agent is probably necessary for the latter application. Bring
to room temperature, vortex vigorously, DO NOT centrifuge. Treat As If
Containing Infectious Agents. Antigen should be titrated to determine
optimal concentrations for a particular application but suggest 2-10 ug/well
for direct ELISA |
| Specification |
2.8 mg/ml protein by Bradford Method |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
999999 |
| Item |
Dengue Viral Antigen Type 3 |
|
| Host |
No Host |
| Catalog Number |
ADV3-55 |
| Quantity |
Liquid |
| Physical State |
Tris-Borate-Saline pH 9 |
| Preservative |
4 degrees for short term: -70 for long term. Do not
repeatedly freeze-thaw |
| Storage |
Dengue 1,2,3, and 4 viruses were grown in C6/36
Ae.albopictus cells. Virus was concentrated by 7% PEG precipitation,
inactivated with Betapropiolactone for 48 hours and resuspended in
Tris-borate-saline. Inactivation is confirmed by culture and FA
techniques |
| Procedure |
Contains about 32 HA units of antigen. Can be used
in direct and IgM capture ELISA. Bring to room temperature, vortex
vigorously, DO NOT centrifuge. Treat As If Containing Infectious Agents.
Antigen should be titrated to determine optimal concentrations for a
particular application but suggest 2-10 ug/well for direct ELISA |
| Specification |
2.47 mg/ml total protein by Bradford Method |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
999999 |
| Item |
Dengue Viral Antigen Type 4 |
|
| Host |
No Host |
| Catalog Number |
ADV4-55 |
| Quantity |
Liquid |
| Physical State |
Tris-Borate-Saline pH 9 |
| Preservative |
4 degrees for short term: -70 for long term. Do not
repeatedly freeze-thaw |
| Storage |
Dengue 1,2,3, and 4 viruses were grown in C6/36
Ae.albopictus cells. Virus was concentrated by 7% PEG precipitation,
inactivated with Betapropiolactone for 48 hours and resuspended in
Tris-borate-saline. Inactivation is confirmed by culture and FA
techniques |
| Procedure |
Contains about 32 HA units of antigen. Can be used
in direct and IgM capture ELISA. Bring to room temperature, vortex
vigorously, DO NOT centrifuge. Treat As If Containing Infectious Agents.
Antigen should be titrated to determine optimal concentrations for a
particular application but suggest 2-10 ug/well for direct ELISA |
| Specification |
2.8 mg/ml protein by Bradford Method |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
999999 |
| Item |
Leishmania Antigens |
|
| Host |
No Host |
| Catalog Number |
ALEI-55 |
| Quantity |
1.0 mg |
| Physical State |
Lyophilized |
| Buffer |
Reconstitute with PBS pH 7.4, |
| Preservative |
store aliquots at -20C |
| Storage |
4C |
| Procedure |
Promastigotes grown in Schneiders medium plus 20%
FCS at 25C, harvested by centrifugation, washed with PBS, disrupted by
sonication. Soluble proteins were obtained from the supernatant after
high speed centrifugation and the supernatant lyophilized |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For ELISA, antigen resuspended in 0.5ml PBS pH 7.4.
Immulon 2 plates can be sensitized with 1-10 ug of antigen per well. For
Western blot, antigen can be diluted in a loading buffer of 50mM
Tris-HCl, 100mM mercaptoethanol, 2% SDS, 0.1% Bromophenol blue and 10%
glycerol |
| Item |
Plasmodium falciparum HRP II Antigen |
|
| Host |
No Host |
| Catalog Number |
AGPF-55 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
20 mM Carbonate Buffer pH 9.6 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C short term; -20 |
| Procedure |
The recombinant protein is produced in E.coli using
recombinant technology |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
Greater than 90% purity.Functions well in ELISA and
Immunochromatographic procedures |
|
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+32 (0) 16
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