|
| |
Epitope/Affinity Tags and Fusion Proteins Spec
| Item |
12-tag Protein Marker |
|
| Catalog Number |
45-LYS-1 |
| Quantity |
0.2 ml |
| Physical State |
Liquid |
| Buffer |
1X reduced SDS-PAGE sample loading buffer. |
| Storage |
4C short term |
| Procedure |
Bacteria were lysed with SDS-PAGE sample loading
dye, boiled at 95C for 10 minutes, then centrifuged for 13,000 g for 15
minutes. |
| Specification |
This E.coli lysate contains a 41kd protein
expressing commonly used tags which are fused in the order of GST - T7 -
HSV- C-MYC - VSV - Glu-Glu - V5 - E-tag - DYKDDDDK - S-tag - HA - 6xHIS.
Protein concentration is 0.5mg/ml. |
| Western Blots |
0.5ul - 4.0 ul |
| ELISA |
untested |
| Application |
Positive control for western blotting with epitope
tags and fusion tags. Recommend loading 0.5ul - 4.0ul of cell lysate for
10-12% gels. |
| Item |
Affinity Purified Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freund's adjuvant eggs were
collected and the immunoglobulin fraction prepared. Anti-6-HIS was
immunoaffinity purified using the peptide immobilized on a solid support |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with 6xHIS conjugated with
KLH. After multiple immunizations in Freund's adjuvant eggs were
collected and the immunoglobulin fraction prepared. Anti-6-HIS
antibodies were immunoaffinity purified using the peptide immobilized on
a solid support |
| Specification |
Suggested starting dilution for anti-6-HIS: IF =
1:500 Flow cytometry = 1:1000 |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant eggs were
collected and the immunoglobulin fraction prepared. Anti-6-HIS was
immunoaffinity purified using the peptide immobilized on a solid
support. |
| Specification |
Anti-6-HIS molar F/P ratio is 3-6. |
| Western Blots |
1:1,000-1:5,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freund's adjuvant eggs were
collected and the immunoglobulin fraction prepared. Anti-6-HIS was
immunoaffinity purified using the peptide immobilized on a solid
support. |
| Specification |
The anti-6-HIS molar F/P ratio is 3-6. |
| Western Blots |
1:1,000-1:5,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with HRP (Horseradish Peroxidase) |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-6-HIS |
|
| Host |
Chicken |
| Catalog Number |
CHIS-45P |
| Quantity |
1.0mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with HRP (Horseradish Peroxidase) |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS antibodies were immunoaffinity purified using the
peptide immobilized on a solid support |
| Specification |
Anti-6-HIs for IF assays =1:500. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS was immunoaffinity purified using the peptide
immobilized on a solid support. |
| Specification |
For use in IF studies recommended starting dilution
for anti-6-HIS = 1:500 |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS antibodies were immunoaffinity purified using the
peptide immobilized on a solid supportand conjugated with biotin. |
| Specification |
|
| Western Blots |
1:1,000-1:5,000. |
| ELISA |
1:2,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS antibodies were immunoaffinity purified using the
peptide immobilized on a solid supportand conjugated with biotin. |
| Specification |
|
| Western Blots |
1:1,000-1:5,000. |
| ELISA |
1:2,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS antibodies were immunoaffinity purified using the
peptide immobilized on a solid supportand conjugated with fluorescein
isothiocyanate (FITC). |
| Specification |
anti-6-HIS for IF = 1:250-1:500 The molar F/P ratio
is 3.7 |
| Western Blots |
1:1,000-1:5,000. |
| ELISA |
1:2,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS antibodies were immunoaffinity purified using the
peptide immobilized on a solid support and conjugated with fluorescein
isothiocyanate (FITC). |
| Specification |
Anti-6-HIS for IF = 1:250-1:500. The molar F/P ratio
is 3.7 |
| Western Blots |
1:1,000-1:5,000. |
| ELISA |
1:2,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS was immunoaffinity purified using the peptide
immobilized on a solid support then conjugated with horseradish
peroxidase (HRP) |
| Specification |
Anti-6-HIS for Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-6-HIS |
|
| Host |
Rabbit |
| Catalog Number |
RHIS-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HHHHHH conjugated with
KLH. After multiple immunizations in Freunds adjuvant serum was
collected. Anti-6-HIS was immunoaffinity purified using the peptide
immobilized on a solid support and conjugated with horseradish
peroxidase (HRP) |
| Specification |
Anti-6-HIS for Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-AU1 |
|
| Host |
Chicken |
| Catalog Number |
CAU-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with AU1 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and an IgY fraction was prepared. Antibodies were then
immunoaffinity purified using the peptide immobilized on a solid phase |
| Specification |
Recommended dilution range for IF and IHC:
1:250-1:1000. Immunoprecipitation 1:250-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-AU1 |
|
| Host |
Chicken |
| Catalog Number |
CAU-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with AU1 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and an IgY fraction was prepared. Antibodies were then
immunoaffinity purified using the peptide immobilized on a solid phase |
| Specification |
Recommended dilution range for IF and IHC:
1:250-1:1000. Immunoprecipitation 1:250-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-AU1 |
|
| Host |
Rabbit |
| Catalog Number |
RAU-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with AU1 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
Recommended dilution range for IF and IHC:
1:250-1:1000. Immunoprecipitation 1:250-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-AU1 |
|
| Host |
Rabbit |
| Catalog Number |
RAU-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with AU1 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
Recommended dilution range for IF and IHC:
1:250-1:1000. Immunoprecipitation 1:250-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-beta
Galactosidase |
|
| Host |
Chicken |
| Catalog Number |
CGAL-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Immunofluorescence |
2 ug/ml |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, eggs were collected and delipidated. Antibodies
were solid phase absorbed then immunoaffinity purified using purified
beta-galactosidase immobilized on a solid phase. The anti-beta
galactosidase concentration was determined by extinction coefficient :
O.D. 1.32 at 280nM equals 1.0 mg |
| Specification |
Recommended starting dilution of anti-beta
galactosidase for IF is 2 ug/ml. Less than 2% reactivity with E. Coli. |
| Western Blots |
1:500 - 1:2,000. |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-beta
Galactosidase |
|
| Host |
Chicken |
| Catalog Number |
CGAL-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Immunofluorescence |
2 ug/ml |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, eggs were collected and delipidated. Antibodies
were solid phase absorbed then immunoaffinity purified using purified
beta-galactosidase immobilized on a solid phase. The anti-beta
galactosidase concentration was determined by extinction coefficient :
O.D. 1.32 at 280nM equals 1.0 mg |
| Specification |
Recommended starting dilution of anti-beta
galactosidase for IF is 2 ug/ml. Less than 2% reactivity with E. Coli. |
| Western Blots |
1:500 - 1:2,000. |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-beta
Galactosidase |
|
| Host |
Chicken |
| Catalog Number |
CGAL-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES - Saline pH 7.2 - 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, eggs were collected and delipidated. The anti-beta
galactosidase was solid phase absorbed, immunoaffinity purified and
conjugated with biotin. |
| Specification |
|
| Western Blots |
1:1,000 - 1:5,000. |
| ELISA |
1:5,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-beta
Galactosidase |
|
| Host |
Chicken |
| Catalog Number |
CGAL-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES - Saline pH 7.2 - 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, eggs were collected and delipidated. The anti-beta
galactosidase was solid phase absorbed, immunoaffinity purified and
conjugated with biotin. |
| Specification |
|
| Western Blots |
1:1,000 - 1:5,000. |
| ELISA |
1:5,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Immunofluorescence |
2 ug/ml |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. The anti-beta galactosidase
antibodies were solid phase absorbed then immunoaffinity purified using
purified off an antigen containing immunoabsorbant. |
| Specification |
Recommended starting dilution of anti-beta
galactosidase for IF is 2 ug/ml. Less than 2% reactivity with E. Coli. |
| Western Blots |
1:1000 - 1:3,000 |
| ELISA |
capture 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Immunofluorescence |
2 ug/ml |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. Antibodies were solid phase
absorbed then immunoaffinity purified using purified beta-galactosidase
immobilized on a solid phase |
| Specification |
Recommended starting dilution as ELISA detection
antibody is 1:10,000. Less than 2% reactivity with E. Coli. |
| Western Blots |
1:1000 - 1:3,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES - Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. The anti-beta galactosidase
was purified off an antigen containing immunoabsorbant then conjugated
with biotin. |
| Specification |
Prior to conjugation the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses. Less
than 2.0% cross-reactivity with E-coli. |
| Western Blots |
1 ug/ml -0.2 ug/ml |
| ELISA |
0.2 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES - Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. The anti-beta galactosidase
was purified off an antigen containing immunoabsorbant then conjugated
with biotin. |
| Specification |
Prior to conjugation the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses. Less
than 2.0% cross-reactivity with E-coli. |
| Western Blots |
1 ug/ml -0.2 ug/ml |
| ELISA |
0.2 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. Antibodies were solid phase
absorbed, immunoaffinity purified using purified beta-galactosidase
immobilized on a solid phase and conjugated with Fluorescein
Isothiocyanate (FITC) |
| Specification |
Prior to conjugation the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses. Less
than 2.0% cross-reactivity with E-coli. Molar F/P ratio is: 3 ¨C 6. |
| Western Blots |
Recommended dilution range: 1:1000-1:5000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. Antibodies were solid phase
absorbed, immunoaffinity purified using purified beta-galactosidase
immobilized on a solid phase and conjugated with Fluorescein
Isothiocyanate (FITC) |
| Specification |
Prior to conjugation the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses. Less
than 2.0% cross-reactivity with E-coli. Molar F/P ratio is: 3 ¨C 6. |
| Western Blots |
Recommended dilution range: 1:1000-1:5000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. The anti-beta galactosidase
was purified off an antigen containing immunoabsorbant then conjugated
with horseradish peroxidase. |
| Specification |
Antibody specificity was verified by Less than 2%
reactivity with E. Coli. Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-beta
Galactosidase |
|
| Host |
Rabbit |
| Catalog Number |
RGAL-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with purified
beta-galactosidase from Escherichia coli. After multiple immunizations
in Freunds adjuvant, serum was collected. The anti-beta galactosidase
was purified off an antigen containing immunoabsorbant then conjugated
with horseradish peroxidase. |
| Specification |
Less than 2% reactivity with E. Coli.
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45A-Z |
| Quantity |
0.1mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC conjugated with
KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and the anti-C-MYC was immunoaffinity purified off an antigen
containing immunoabsorbant. |
| Specification |
Recommended starting dilution of anti-C-MYC for IF =
1:750 ICC = 1:200-1:500 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
Coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. Anti-C-myc
antibodies were immunoaffinity purified using the peptide immobilized on
a solid phase. |
| Specification |
Recommended starting dilution of anti-C-MYC for IF =
1:750 ICC = 1:200-1:500 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collected and an IgY fraction was prepared. The anti-C-MYC was
affinity purified off a peptide containing immunoabsorbant then
conjugated with biotin. |
| Specification |
For other applications the optimal working dilution
of anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Chicken anti-C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collected and an IgY fraction was prepared. The anti-C-MYC was
affinity purified off a peptide containing immunoabsorbant then
conjugated with biotin. |
| Specification |
For other applications the optimal working dilution
of anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-C-MYC was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with Fluorescein Isothiocyanate (FITC). |
| Specification |
Recommended starting dilution of this anti-C-MYC for
IF = 1:1,000. |
| Western Blots |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-C-MYC was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with Fluorescein Isothiocyanate (FITC). |
| Specification |
Recommended starting dilution of this anti-C-MYC for
IF = 1:1,000. |
| Western Blots |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-C-MYC was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with horseradish peroxidase. |
| Specification |
For other applications, optimal starting dilution of
anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti C-Myc |
|
| Host |
Chicken |
| Catalog Number |
CMYC-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with C-MYC peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-C-MYC was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with horseradish peroxidase. |
| Specification |
For other applications, optimal starting dilution of
anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-C-Myc |
|
| Host |
Goat |
| Catalog Number |
GMYC-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-C-myc antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase. |
| Specification |
Recommended dilution range for anti-C-myc: IF and
IHC= 1:250-1:1000 Immunoprecipitation= 1:250-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Goat anti-C-Myc |
|
| Host |
Goat |
| Catalog Number |
GMYC-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Goats were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-C-myc antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase. |
| Specification |
Recommended dilution range for anti-C-myc: IF and
IHC= 1:250-1:1000 Immunoprecipitation= 1:250-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-C-myc antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase. |
| Specification |
Recommended dilution range for anti-C-myc: IF and
IHC= 1:250-1:1000 Immunoprecipitation= 1:250-1:500 |
| Western Blots |
1:2,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant). |
| Specification |
Recommended dilution range for anti-C-myc: IF and
IHC= 1:250-1:1000 Immunoprecipitation= 1:250-1:500 |
| Western Blots |
1:2,000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-C-MYC |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-145D-Z |
| Quantity |
0.2 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody Content |
0.1 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-C-MYC was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-C-MYC was then coupled to an agarose gel. |
| Specification |
The anti-C-MYC reacts with both N-terminal and
C-terminal MYC tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-C-MYC |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-145D |
| Quantity |
2.0 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody Content |
1.0 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-C-MYC was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-C-MYC was then coupled to an agarose gel. |
| Specification |
The anti-C-MYC reacts with both N-terminal and
C-terminal MYC tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant then conjugated with biotin. |
| Specification |
For other applications the optimal working dilution
of anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant then conjugated with biotin. |
| Specification |
For other applications the optimal working dilution
of anti-C-MYC should be determined by each investigator. |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant then conjugated with fluorescein isothiocyanate (FITC). |
| Specification |
We recommend diluting this anti-C-MYC at 1:250 for
IF applications. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant then conjugated with fluorescein isothiocyanate (FITC). |
| Specification |
We recommend diluting this anti-C-MYC at 1:250 for
IF applications. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was affinity purified off a peptide containing
immunoabsorbant then conjugated with horseradish peroxidase. |
| Specification |
Optimal working dilutions for the anti-C-MYC should
be determined for your particular assay conditions. Immunocytochemistry
= 1:200-1:500 |
| Western Blots |
1:1000-1:5000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-C-Myc |
|
| Host |
Rabbit |
| Catalog Number |
RMYC-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with C-MYC peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-C-MYC was immunoaffinity purified off a peptide
containing immunoabsorbant then conjugated with horseradish peroxidase |
| Specification |
Optimal anti-C-MYC working dilutions should be
determined for your particular assay conditions. Immunocytochemistry =
1:200-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit
anti-Calmodulin Binding Protein |
|
| Host |
Rabbit |
| Catalog Number |
RCBP-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with synthetic peptide
corresponding to the Calmodulin Binding Protein (CBP) epitope tag
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
serum was collected. The anti-calmodulin binding protein (CBP) were
immunoaffinity purified using the peptide immobilized on a solid phase. |
| Specification |
Optimal working dilutions for anti-calmodulin
binding peptide (CBP) should be determined for your particular
application. |
| Western Blots |
1 ug/ml - 0.2 ug/ml |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit
anti-Calmodulin Binding Protein |
|
| Host |
Rabbit |
| Catalog Number |
RCBP-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with synthetic peptide
corresponding to the Calmodulin Binding Protein (CBP) epitope tag
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
serum was collected. The anti-calmodulin binding protein (CBP) were
immunoaffinity purified using the peptide immobilized on a solid phase. |
| Specification |
Optimal working dilutions for anti-calmodulin
binding peptide (CBP) should be determined for your particular
application. |
| Western Blots |
1 ug/ml - 0.2 ug/ml |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with the DYKDDDDK peptide
conjugated with KLH. After several immunizations, eggs were collected
and the Ig fraction prepared. Anti-DYKDDDDK antibodies were
immunoaffinity purified using peptide coupled to a solid phase support. |
| Western Blots |
1:2,000 - 1:10,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with the DYKDDDDK peptide
conjugated with KLH. After several immunizations, eggs were collected
and the Ig fraction prepared. Anti-DYKDDDDK antibodies were
immunoaffinity purified using peptdie coupled to a solid phase support. |
| Western Blots |
1:2,000 - 1:10,000 |
| ELISA |
1:5,000 - 1:10,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2. |
| Preservative |
0.1% sodium azide |
| Immunofluorescence |
1:500-1:2000 |
| Storage |
4 C |
| Procedure |
Chickens were immunized with the DYKDDDDK peptide
conjugated with KLH. After several immunizations, eggs were collected
and the Ig fraction prepared. Anti-DYKDDDDK antibodies were
immunoaffinity purified using the peptide immobilized on a solid phase
matrix then conjugated with Fluorescein Isothiocyanate (FITC) |
| Specification |
The molar F/P ratio of this anti-DYKDDDDK is 4.1 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2. |
| Preservative |
0.1% sodium azide |
| Immufluorescence |
1:1,000 |
| Storage |
4 C |
| Procedure |
Chickens were immunized with the DYKDDDDK peptide
conjugated with KLH. After several immunizations, eggs were collected
and the Ig fraction prepared. Anti-DYKDDDDK antibodies were
immunoaffinity purified using the peptide immobilized on a solid phase
matrix then conjugated with Fluorescein Isothiocyanate (FITC) |
| Specification |
The molar F/P ratio of this anti-DYKDDDDK is 4.1 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with DYKDDDDK peptide
conjugated with KLH. After multiple immunizations in Freund's adjuvant,
eggs were collected. Anti-DYKDDDDK antibodies were immunoaffinity
purified using the peptide conjugated to a solid-phase support then
conjugated to HRP |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-DYKDDDDK |
|
| Host |
Chicken |
| Catalog Number |
CFLG-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with DYKDDDDK peptide
conjugated with KLH. After multiple immunizations in Freund's adjuvant,
eggs were collected. Anti-DYKDDDDK antibodies were immunoaffinity
purified using the peptide conjugated to a solid-phase support then
conjugated to HRP |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected and the anti-DYKDDDDK antibodies were affinity purified using
the peptide immobilized on a solid phase. |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected and the antibodies were affinity purified using the peptide
immobilized on a solid phase. |
| Western Blots |
1:5000 - 1:10,000 |
| ELISA |
1:5000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with the DYKDDDDK peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
serum was collected. Antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase then conjugated with biotin. |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.0 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected and the antibodies were affinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with Fluorescein Isothiocyanate (FITC) |
| Specification |
The molar F/P ratio is 4.1 Recommended starting
dilution for IF = 1:250 - 1:1000. |
| Western Blots |
1:1000 - 1:5000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.0 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected and the antibodies were affinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with Fluorescein Isothiocyanate (FITC) |
| Specification |
The molar F/P ratio is 4.1 |
| Western Blots |
1:1000-1:5000 |
| ELISA |
1:5000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected and the antibodies were affinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with horseradish peroxidase. |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-DYKDDDDK |
|
| Host |
Rabbit |
| Catalog Number |
RFLG-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with DYKDDDDK peptide
conjugated to KLH. After multiple immunizations in Freund's adjuvant,
serum was collected and the antibodies were affinity purified using the
peptide immobilized on a solid phase. The affinity purified antibodies
were then conjugated with horseradish peroxidase. |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-E-tag |
|
| Host |
Chicken |
| Catalog Number |
CET-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and an IgY fraction was prepared. The anti-E-tag was affinity
purified off a peptide containing immunoabsorbant. |
| Western Blots |
1 ug/ml to 0.2 ug/ml |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-E-tag |
|
| Host |
Chicken |
| Catalog Number |
CET-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and an IgY fraction was prepared. The anti-E-tag was
immunoaffinity purified off a peptide containing immunoabsorbant. |
| Western Blots |
1 ug/ml to 0.2 ug/ml |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-E-tag |
|
| Host |
Chicken |
| Catalog Number |
CET-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-E-tag was immunoaffinity purified off a peptide
containing immunoabsorbant then conjugated with horseradish peroxidase
(HRP). |
| Western Blots |
1:1,000 - 1:5,000. |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
ELISA: Antibody specificity was verified by ELISA
against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution
of the antibody gave an O.D.=1.0 in a 15 minute reaction using ICL, Inc
HRPO conjugated donkey anti rabbit (IgG) at 1:18,000 and TMB as the
substrate. Appropriate specificity controls were run. |
| Western Blots |
Recommended dilution range 1:1,000 ¨C 1:3,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
ELISA: Antibody specificity was verified by ELISA
against the peptide conjugated to BSA (E-tag-BSA). A 1:10,000 dilution
of the antibody gave an O.D.=1.0 in a 15 minute reaction using ICL, Inc
HRPO conjugated donkey anti rabbit (IgG) at 1:18,000 and TMB as the
substrate. Appropriate specificity controls were run. |
| Western Blots |
Recommended dilution range 1:1,000 ¨C 1:3,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.0 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with Fluorescein Isothiocyanate (FITC) |
| Specification |
Prior to conjugation, the antibodies were analyzed
by ELISA and Western Blot.The molar F/P ratio = 3 ¨C 6 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.0 with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with E-tag conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-E-tag was immunoaffinity purified using the peptide
immobilized on a solid phase then conjugated with Fluorescein
Isothiocyanate (FITC). |
| Specification |
The molar F/P ratio = 3 ¨C 6 |
| Western Blots |
1 ug/ml |
| ELISA |
1 ug/ml - 0.2 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase and conjugated with HRP |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:2,000 - 1:10,000. |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti E-tag |
|
| Host |
Rabbit |
| Catalog Number |
RET-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase and conjugated with HRP. |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:2,000 - 1:10,000. |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-GFP |
|
| Host |
Rabbit |
| Catalog Number |
RGFP-45A |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
TBS pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified
green-fluorescent protein (GFP) isolated directly from Aequorea
victoria. Multiple immunizations in Freunds adjuvant, serum was
collected. Affinity purified anti-GFP was isolated using the native GFP
immobilized on a solid phase. |
| Specification |
This anti-GFP recognizes all variants of recombinant
Aequorea green-fluorescent protein. Suggested working dilutions: Elisa:
1;10,000-1:100,000 Blotting: 1:5,000-1:15,000 Working dilutions must be
determined by individual investigator but antibodies have been used with
success for Elisa, Dot Blot, Western Blot, and Immunoprecipitation. |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:2,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-GFP |
|
| Host |
Rabbit |
| Catalog Number |
RGFP-45F |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS,pH 7.2 with 0.2% BSA. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with highly purified
green-fluorescent protein (GFP) isolated directly from Aequorea
victoria. Multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the native GFP
immobilized on a solid phase. The resulting anti-GFP was conjugated with
fluorescein isothiocyanate (FITC). |
| Specification |
ICL's anti-GFP recognizes all variants of
recombinant Aequorea green-fluorescent protein. Molar F/P ratio = 3.5 |
| Western Blots |
1:500-1:2,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti- Glu-Glu |
|
| Host |
Chicken |
| Catalog Number |
CGLU-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2. |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with Glu-Glu conjugated with
KLH. After multiple immunizations in Freund¡¯s adjuvant, the eggs were
collected and an IgY fraction was prepared. The antibodies were
immunoaffinity isolated using peptide conjugated with a different
carrier |
| Specification |
Antibody activity was verified using peptide coupled
with different carrier.Elisa: 1:10,000-1:100,000 Antibody activity was
verified using peptide coupled with different carrier.Blotting:
1:1000-1:3000. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti- Glu-Glu |
|
| Host |
Rabbit |
| Catalog Number |
RGLU-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with Glu-Glu (EYMPME)
conjugated with KLH. After multiple immunizations in Fruend¡¯s adjuvant
serum was collected and the antibodies immunoaffinity isolated using
peptide conjugated with a different carrier |
| Specification |
Antibody activity was verified by Elisa and Western
Blot.ELISA: Recommended dilution range: Capture: 1:200-1:500 Primary:
1:1,000-1:10,000 |
| Western Blots |
Recommended dilution range: 1:5001:2500 Optimal
working dilutions should be determined by researcher |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti- Glu-Glu |
|
| Host |
Rabbit |
| Catalog Number |
RGLU-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with Glu-Glu (EYMPME)
conjugated with KLH. After multiple immunizations in Fruend¡¯s adjuvant
serum was collected and the antibodies immunoaffinity isolated using
peptide conjugated with a different carrier |
| Specification |
Antibody activity was verified by Elisa and Western
Blot.ELISA: Recommended dilution range: Capture: 1:200-1:500 Primary:
1:1,000-1:10,000 |
| Western Blots |
Recommended dilution range: 1:5001:2500 Optimal
working dilutions should be determined by researcher |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-GST |
|
| Host |
Chicken |
| Catalog Number |
CGST-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant eggs were collected and an
IgY fraction was prepared. The anti-GST antibodies were immunoaffinity
purified using GST immobilized on a solid support. |
| Specification |
For detection antibody in ELISA assays use anti-GST
at 1:5,000 - 1:20,000. |
| Western Blots |
1:1:000 - 1;10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-GST |
|
| Host |
Chicken |
| Catalog Number |
CGST-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant eggs were collected and an
IgY fraction was prepared. The anti-GST antibodies were immunoaffinity
purified using GST immobilized on a solid support. |
| Specification |
For detection antibody in ELISA assays use anti-GST
at 1:5,000 - 1:20,000. |
| Western Blots |
1:1:000 - 1;10,000 |
| ELISA |
1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Publications |
Fischer et al. J. Neurosci..2004; 24: 8726-8740. |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected.
Anti-GST antibodies were immunoaffinity purified using GST immobilized
on a solid support. |
| Specification |
For detection antibody in ELISA assays use anti-GST
at 1:5,000 - 1:20,000. |
| Western Blots |
1:1:000 - 1;10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Publications |
Fischer et al. J. Neurosci..2004; 24: 8726-8740. |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected.
Antibodies were immunoaffinity purified using GST immobilized on a solid
support. |
| Specification |
Western:1:5000-1:10,000 For use as detection
antibody for ELISA use at 1:5,000 - 1:20,000. |
| Western Blots |
1:5000 - 1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Hepes Saline with 0.2% BSA. |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected.
Antibodies were immunoaffinity purified using GST immobilized on a solid
support and conjugated with biotin. |
| Specification |
Blotting 1:2000 Elisa 1:10,000 |
| Western Blots |
1:2000 - 1:10,000 |
| ELISA |
1:5000 - 1:20000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA. |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected. The
anti-GST was immunoaffinity purified using GST immobilized on a solid
support then conjugated with FITC |
| Specification |
Molar F/P ratio = 6.0 IF: 1:1,000 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA. |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected. The
anti-GST was immunoaffinity purified using GST immobilized on a solid
support then conjugated with FITC |
| Specification |
Molar F/P ratio of anti-GST = 6.0 IF: 1:1,000 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected. The
anti-GST antibodies were immunoaffinity purified using GST immobilized
on a solid support and conjugated with HRP |
| Specification |
For Immunocytochemistry use anti-GST at 1:200-1:500
|
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:10,000 - 1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-GST |
|
| Host |
Rabbit |
| Catalog Number |
RGST-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with
Glutathione-S-transferase (GST) from Schistosoma japonicum. After
multiple immunizations in Freund's adjuvant serum was collected.
Anti-GST antibodies were immunoaffinity purified using GST immobilized
on a solid support and conjugated with HRP |
| Specification |
Immunocytochemistry use anti-GST at 1:200-1:500
|
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:10,000 - 1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-HA |
|
| Host |
Chicken |
| Catalog Number |
CHGT-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Anti-HA antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support |
| Specification |
Suggested starting dilution of anti-HA for IF =
1:500
|
| Western Blots |
1:1,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-HA |
|
| Host |
Chicken |
| Catalog Number |
CHGT-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Anti-HA was immunoaffinity purified using the peptide
conjugated to a solid-phase support then conjugated with fluorescein
isothiocyanate (FITC) |
| Specification |
Anti-HA molar F/P ratio is 3-6 |
| Western Blots |
1:1,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-HA |
|
| Host |
Chicken |
| Catalog Number |
CHGT-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Anti-HA was immunoaffinity purified using the peptide
conjugated to a solid-phase support then conjugated with fluorescein
isothiocyanate (FITC) |
| Specification |
Anti-HA Molar F/P ratio is 3-6 |
| Western Blots |
1:1,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-HA |
|
| Host |
Chicken |
| Catalog Number |
CHGT-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund¡¯s adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with HRP (Horseradish Peroxidase) |
| Specification |
By ELISA, the anti HA diluted 1:10,000 gave an O.D.=
1.0 in a 15 minute reaction against peptide conjugated to a different
carrier than used for anti peptide purification. |
| Western Blots |
The Chicken anti-HA was an effective probe at 1:1000
dilution |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-HA |
|
| Host |
Chicken |
| Catalog Number |
CHGT-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund¡¯s adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with HRP (Horseradish Peroxidase) |
| Specification |
By ELISA, the anti HA diluted 1:10,000 gave an O.D.=
1.0 in a 15 minute reaction against peptide conjugated to a different
carrier than used for anti peptide purification. |
| Western Blots |
The Chicken anti-HA was an effective probe at 1:1000
dilution |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Anti-HA antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support. |
| Specification |
Suggested anti-HA working dilutions:
immunofluorescence 1:1000. |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Anti-HA antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support. |
| Specification |
Recommended working dilution for anti-HA:
Immunofluorescence is 1:1000. |
| Western Blots |
1:5,000-1:10,000 |
| ELISA |
coating:1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-HA
|
|
| Host |
Rabbit |
| Catalog Number |
RHGT-145D |
| Quantity |
2.0 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody Content |
1.0 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-HA was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-HA was then coupled to an agarose gel. |
| Specification |
The anti-HA reacts with both N-terminal and
C-terminal HA tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-HA
|
|
| Host |
Rabbit |
| Catalog Number |
RHGT-145D-Z |
| Quantity |
0.2 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody Content |
0.1 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-HA was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-HA was then coupled to an agarose gel. |
| Specification |
The anti-HA reacts with both N-terminal and
C-terminal HA tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with biotin. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with biotin. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 with 0.2% BSA. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with FITC (Fluorescein isothiocyanate) |
| Specification |
Immunofluorescence: 1:250-1:1000. Molar F/P ratio=
3.7 |
| Western Blots |
1:2000-1:10,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 with 0.2% BSA. |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support and the antibodies were conjugated
with FITC (Fluorescein isothiocyanate) |
| Specification |
Immunofluorescence: 1:250-1:1000. Molar F/P ratio=
3.7 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support then conjugated with HRP (
Horseradish Peroxidase) |
| Specification |
Recommended starting dilutions for:
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1000-1:10,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-HA |
|
| Host |
Rabbit |
| Catalog Number |
RHGT-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with HA-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support then conjugated with HRP (
Horseradish Peroxidase) |
| Specification |
Recommended starting dilution for:
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-HSV-tag |
|
| Host |
Chicken |
| Catalog Number |
CSV-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HSV-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support. |
| Specification |
IF = 1:1,000 |
| Western Blots |
1:5,000 dilution |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-HSV-tag |
|
| Host |
Chicken |
| Catalog Number |
CSV-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with HSV-tag conjugated to
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support. |
| Specification |
IF = 1:1,000 |
| Western Blots |
1:5,000 dilution |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with QPELAPEDPEDC conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
ELISA: Antibody specificity was verified by direct
ELISA against the peptide conjugated to BSA (QPELAPEDPEDC-BSA). A
1:8,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute
reaction using ICL, Inc HRPO conjugated donkey anti rabbit (IgG) at
1:18,000 and TMB as the substrate |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with QPELAPEDPEDC conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
ELISA: Antibody specificity was verified by direct
ELISA against the peptide conjugated to BSA (QPELAPEDPEDC-BSA). A
1:8,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute
reaction using ICL, Inc HRPO conjugated donkey anti rabbit (IgG) at
1:18,000 and TMB as the substrate |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with FITC (Fluorescein isothiocyanate) |
| Specification |
Prior to conjugation, the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses.Molar F/P
ratio= 3.7 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with FITC (Fluorescein isothiocyanate) |
| Specification |
Prior to conjugation, the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses.Molar F/P
ratio= 3.7 |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with horseradish peroxidase |
| Specification |
Prior to conjugation, the affinity purified
antibodies were analyzed using ELISA and Western Blot
analyses.Recommended dilution range: 1:1000-1:3000. |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-HSV-tag |
|
| Host |
Rabbit |
| Catalog Number |
RSV-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. The affinity purified antibodies were then
conjugated with horseradish peroxidase |
| Specification |
Prior to conjugation, the affinity purified
antibodies were analyzed using ELISA and Western Blot
analyses.Recommended dilution range: 1:1000-1:3000. |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken
anti-Luciferase |
|
| Host |
Chicken |
| Catalog Number |
CLUC-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4C |
| Procedure |
Chickens were immunized with purified luciferase
derived from Photinus pyralis (firefly). The IgY fraction from
hyperimmune egg yolk was prepared using delipidation and salt
fractionation procedures. The anti-luciferase was then immunoaffinity
purified off an antigen containing immunoabsorbant. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken
anti-Luciferase |
|
| Host |
Chicken |
| Catalog Number |
CLUC-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4C |
| Procedure |
Chickens were immunized with purified luciferase
derived from Photinus pyralis (firefly). The IgY fraction from
hyperimmune egg yolk was prepared using delipidation and salt
fractionation procedures. The anti-luciferase was then immunoaffinity
purified off an antigen containing immunoabsorbant. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Chicken anti-Luciferase |
|
| Host |
Chicken |
| Catalog Number |
CLUC-45G |
| Quantity |
1.0 ml |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4C |
| Procedure |
Chickens were immunized with purified luciferase
derived from Photinus pyralis (firefly). The IgY fraction from
hyperimmune egg yolk was prepared using delipidation and salt
fractionation procedures. |
| Specification |
Concentration of purified IgY is 10.0 mg/ml |
| Western Blots |
1:500-1:2,000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Maltose
Binding Protein |
|
| Host |
Rabbit |
| Catalog Number |
RMBP-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with recombinant maltose
binding protein. After multiple immunizations in Freund's adjuvant serum
was collected. Anti-maltose binding protein antibodies were
immunoaffinity purified using MBP immobilized on a solid support. |
| Specification |
The anti-maltose binding protein works as an
effective probe for ELISA at a 1:25,000 dilution. |
| Western Blots |
1:5000 - 1:10,000 |
| ELISA |
capture 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Maltose
Binding Protein |
|
| Host |
Rabbit |
| Catalog Number |
RMBP-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized withrecombinant maltose
binding protein. After multiple immunizations in Freund¡¯s adjuvant
serum was collected. Antibodies were immunoaffinity purified using MBP
immobilized on a solid support. |
| Specification |
The anti-MBP works as an effective probe for ELISA
at a 1:25,000 dilution. |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
1:5000 - 1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Protein C
|
|
| Host |
Rabbit |
| Catalog Number |
RPC-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
Antibody specificity was verified by ELISA against
the peptide conjugated to BSA (Protein C tag-BSA). A 1:15,000 dilution
of the antibody gave an O.D.=1.0 in a 15 minute reaction using ICL, Inc
HRPO conjugated donkey anti rabbit (IgG) at 1:18,000 and TMB as the
substrate. |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:100-1:400 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-Protein C
|
|
| Host |
Rabbit |
| Catalog Number |
RPC-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with peptide conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were then immunoaffinity purified off an antigen
containing immunoabsorbant. |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:100-1:400 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2. |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier |
| Specification |
Recommended dilution range for ELISA as primary
antibody: 1:1,000 - 1:20,000. Blotting: 1:1000-1:5000. |
| Western Blots |
up to 1:5000 |
| ELISA |
Coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2. |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier |
| Specification |
Recommended dilution range for ELISA as primary
antibody: 1:1,000 - 1:20,000. Blotting: 1:1000-1:5000. |
| Western Blots |
up to 1:5000 |
| ELISA |
Coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier. The anti-S-tag antibodies were then conjugated
with fluorescein isothiocyante (FITC). |
| Specification |
Molar F/P ratio: 3-6. |
| Western Blots |
1:1,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier. The anti-S-tag antibodies were then conjugated
with fluorescein isothiocyante (FITC). |
| Specification |
Molar F/P ratio: 3-6. |
| Western Blots |
1:1,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier. The anti-S-tag antibodies were then conjugated
with horseradish peroxidase HRP. |
| Western Blots |
1 ug/ml - 0.2 ug/ml |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-S-tag |
|
| Host |
Rabbit |
| Catalog Number |
RES-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with S-tag conjugated with
KLH. After multiple immunizations in Freund's adjuvant, the rabbits were
bled and the antibodies immunoaffinity isolated using peptide conjugated
with a different carrier. The anti-S-tag antibodies were then conjugated
with horseradish peroxidase HRP. |
| Western Blots |
1 ug/ml to 0.2 ug/ml |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-T7 |
|
| Host |
Chicken |
| Catalog Number |
CT7-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with T7 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-T7 antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase |
| Specification |
Use anti-T7 at 1:10,000-1:25,000 as detection
antibody in ELISA assays. Immunofluorescence use at 1:500 - 1:3,000 |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-T7 |
|
| Host |
Chicken |
| Catalog Number |
CT7-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with T7 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-T7 antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase. |
| Specification |
Use anti-T7 at 1:10,000-1:25,000 as detection
antibody in ELISA assays. Immunofluorescence use at 1:500-1:3,000 |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 conjugated to KLH.
After multiple immunizations in Freund's adjuvant, serum was collected.
Anti-T7 antibodies were immunoaffinity purified using the peptide
conjugated to a solid-phase support. |
| Specification |
For ELISA detection antibody use anti-T7 at
1:5,000-1:20,000. |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 peptide conjugated to
KLH. After multiple immunizations in Freund's adjuvant, serum was
collected. Anti-T7 antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support |
| Specification |
For IF; recommended starting dilution of anti-T7 is
1:300. Optimal working dilutions should be determined for each
particular assay system. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide. |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 epitope tag
conjugated to KLH. After multiple immunizations in Freund's adjuvant,
serum was collected. Anti-T7 antibodies were immunoaffinity purified
using the peptide conjugated to a solid-phase support and conjugated
with FITC (Fluorescein isothiocyanate) |
| Specification |
Recommended starting dilution of anti-T7 for IF:
1:200- 1:500. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 with 0.2% BSA |
| Preservative |
0.1% sodium azide. |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 epitope tag
conjugated to KLH. After multiple immunizations in Freund¡¯s adjuvant,
serum was collected. Antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support and conjugated with FITC
(Fluorescein isothiocyanate) |
| Specification |
The affinity purified antibodies were analyzed using
ELISA and Western Blot analyses.Blotting: Recommended dilution range of
1:1000-1:3000.IF: 1:200- 1:500 |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 epitope tag
conjugated to KLH. After multiple immunizations in Freund's adjuvant,
serum was collected. Antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support and conjugated with
horseradish peroxidase |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:5,000 - 1:10,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-T7 tag |
|
| Host |
Rabbit |
| Catalog Number |
RT7-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with T7 epitope tag
conjugated to KLH. After multiple immunizations in Freund's adjuvant,
serum was collected. Antibodies were immunoaffinity purified using the
peptide conjugated to a solid-phase support and conjugated with
horseradish peroxidase |
| Specification |
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken
anti-Thioredoxin |
|
| Host |
Chicken |
| Catalog Number |
CRDX-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified Thioredoxin
from Escherichia coli. After multiple immunizations in Freunds adjuvant,
eggs were collected and delipidated. Anti-thioredoxin was immunoaffinity
purified using an antigen containing immunoabsorbant. |
| Specification |
Recommended starting dilution as ELISA detection
antibody is 1:10,000. |
| Western Blots |
1:1,000 - 1:10,000. |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken
anti-Thioredoxin |
|
| Host |
Chicken |
| Catalog Number |
CRDX-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with purified Thioredoxin
from Escherichia coli. After multiple immunizations in Freunds adjuvant,
eggs were collected and delipidated. Anti-thioredoxin was immunoaffinity
purified using an antigen containing immunoabsorbant. |
| Specification |
Recommended starting dilution as ELISA detection
antibody is 1:10,000. |
| Western Blots |
1:1,000 - 1:10,000. |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with peptide conjugated with
KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
Recommended working dilutions for this anti-V5 is
1:10,000-1:40,000 when used as a detection antibody in ELISA assays. |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freund's adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase |
| Specification |
Recommended working dilutions for this anti-V5 is
1:10,000-1:40,000 when used as a detection antibody in ELISA assays. |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase then conjugated with Fluorescein
Isothiocyanate (FITC) |
| Specification |
Anti-V5 molar F/P ratio is: 3-6 |
| Western Blots |
1:1,000 |
| ELISA |
1:1,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase then conjugated with Fluorescein
Isothiocyanate (FITC) |
| Specification |
Anti-V5 molar F/P ratio is: 3-6 |
| Western Blots |
1:1,000 |
| ELISA |
1:1,000-1:10,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase and conjugated with horseradish peroxidase |
| Specification |
By ELISA, the anti-V5 diluted 1:10,000 gave an O.D.=
1.0 in a 15 minute reaction against peptide conjugated to a different
carrier than used for anti peptide purification |
| Western Blots |
1:1,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti-V5 |
|
| Host |
Chicken |
| Catalog Number |
CV5-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected. Anti-V5 was immunoaffinity purified using the peptide
immobilized on a solid phase and conjugated with horseradish peroxidase |
| Specification |
By ELISA, the anti-V5 diluted 1:10,000 gave an O.D.=
1.0 in a 15 minute reaction against peptide conjugated to a different
carrier than used for anti peptide purification |
| Western Blots |
1:1,000 |
| ELISA |
1:5,000-1:20,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5 peptdie conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-V5 antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase |
| Specification |
For IF recommended starting dilution for anti-V5 is
1:1,0000. As detection antibody for ELISA use anti-V5 at
1:5,000-1:20,000. |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase. |
| Specification |
IF = suggest using anti-V5 at 1:1,000. ELISA
detection antibody 1:5,000-1:20,000 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-V5
|
|
| Host |
Rabbit |
| Catalog Number |
RV5-145D-Z |
| Quantity |
0.2 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody content |
0.1 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-V5 was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-V5 was then coupled to an agarose gel. |
| Specification |
The anti-V5 reacts with both N-terminal and
C-terminal V5 tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Rabbit anti-V5
|
|
| Host |
Rabbit |
| Catalog Number |
RV5-145D |
| Quantity |
2.0 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Antibody Content |
1.0 mg |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5-KLH in Freund's
adjuvant. After multiple immunizations, serum was collected and the
anti-V5 was isolated by immunoaffinity chromatography. The resulting
Rabbit anti-V5 was then coupled to an agarose gel. |
| Specification |
The anti-V5 reacts with both N-terminal and
C-terminal V5 tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45B-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with the V5 peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
serum was collected. Anti-V5 was immunoaffinity purified using the
peptide immobilized on a solid phase then conjugated with biotin. |
| Specification |
ELISA use anti-V5 at 1:5,000 Blotting
1:1,000-1:3,000 |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Biotin Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45B |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
HEPES SALINE with 0.2% BSA |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with the V5 peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
serum was collected. Anti-V5 was immunoaffinity purified using the
peptide immobilized on a solid phase then conjugated with biotin. |
| Specification |
ELISA use anti-V5 at 1:5,000 Blotting
1:1,000-1:3,000 |
| Western Blots |
1:1,000-1:3,000 |
| ELISA |
1:5,000 - 1:10,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Antibodies were immunoaffinity purified using the peptide
immobilized on a solid phase then conjugated with Fluorescein
Isothiocyanate (FITC). |
| Specification |
Anti-V5 has a molar F/P ratio = 4.2 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with GKPIPNPLLGLDST (V5
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. Antibodies were immunoaffinity purified
using the peptide immobilized on a solid phase then conjugated with
Fluorescein Isothiocyanate (FITC). |
| Specification |
This anti-V5 has a Molar F/P ratio = 4.2 |
| Western Blots |
1:1,000 - 1:5,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-V5 antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase then conjugated with Horseradish
Peroxidase(HRP). |
| Specification |
Optimal working dilutions for anti-V5 conjugate
should be determined for your particular assay conditions.
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000 -1:10,000 |
| ELISA |
1:3,000-15,000. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-V5 |
|
| Host |
Rabbit |
| Catalog Number |
RV5-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with V5 peptide conjugated
with KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. Anti-V5 antibodies were immunoaffinity purified using the
peptide immobilized on a solid phase and conjugated with Horseradish
Peroxidase. |
| Specification |
Optimal conjugate working dilutions for anti-V5
should be determined for your particular assay conditions.
Immunocytochemistry = 1:200-1:500 |
| Western Blots |
1:1,000-1:10,000 |
| ELISA |
1:5,000-1:15,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45A-Z |
| Quantity |
0.1mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g conjugated with
KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and the anti-VSV-g was immunoaffinity purified off an antigen
containing immunoabsorbant. |
| Specification |
For IF use anti-VSV-g at = 1:500 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
Coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g conjugated with
KLH. After multiple immunizations in Freunds adjuvant, eggs were
collected and the anti-VSV-g was immunoaffinity purified off an antigen
containing immunoabsorbant. |
| Specification |
For IF use anti-VSV-g at = 1:500 |
| Western Blots |
1:2,000-1:10,000 |
| ELISA |
Coating: 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Agarose Immobilized Chicken anti-VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-145D-Z |
| Quantity |
0.2 ml of gel |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g-KLH in Freund's
adjuvant. After multiple immunizations, eggs were collected and the
anti-VSV-g was isolated by immunoaffinity chromatography. The resulting
Chicken anti-VSV-g was then coupled to an agarose gel. |
| Specification |
The anti-VSV-g reacts with both N-terminal and
C-terminal VSV tagged proteins via ELISA and Western Blot. |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collected. The anti VSV-g was immunoaffinity purified off a
peptide containing immunoabsorbant then conjugated with Fluorescein
Isothiocyanate (FITC). |
| Specification |
For IF, start using anti-VSV-g at 1:250. |
| Western Blots |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
FITC Conjugated Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collected. The anti VSV-g was immunoaffinity purified off a
peptide containing immunoabsorbant then conjugated with Fluorescein
Isothiocyanate (FITC). |
| Specification |
For IF, start using anti-VSV-g at 1:250. |
| Western Blots |
1:1,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-VSV-g was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with horseradish peroxidase. |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
HRP Conjugated Chicken anti VSV-g |
|
| Host |
Chicken |
| Catalog Number |
CVV-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with VSV-g peptide
conjugated with KLH. After multiple immunizations in Freunds adjuvant,
eggs were collecte and an IgY fraction was prepared. The anti-VSV-g was
immunoaffinity purified using the peptide immobilized on a solid phase
then conjugated with horseradish peroxidase. |
| Western Blots |
1:1,000 - 1:10,000 |
| ELISA |
1:5,000-1:25,000 |
| Application |
For Research and Manufacturing Only; Not For Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-VSV-g |
|
| Host |
Rabbit |
| Catalog Number |
RV V-45A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate Buffered Saline pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with YTDIEMNRLGK (VSV-g
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. The anti-vsv-g antibodies were
immunoaffinity purified using the peptide immobilized on a solid phase |
| Western Blots |
1 ug/ml - 0.1 ug/ml |
| ELISA |
coating 5 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
Affinity Purified Rabbit anti-VSV-g
|
|
| Host |
Rabbit |
| Catalog Number |
RV V-45A-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with YTDIEMNRLGK (VSV-g
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. The anti-VSV-g antibodies were
immunoaffinity purified using the peptide immobilized on a solid phase |
| Western Blots |
1 ug/ml - 0.1 ug/ml |
| ELISA |
coating 5 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-VSV-g |
|
| Host |
Rabbit |
| Catalog Number |
RV V-45F-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with YTDIEMNRLGK (VSV-g
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. Antibodies were immunoaffinity purified
using the peptide immobilized on a solid phase and conjugated with FITC |
| Specification |
Prior to conjugation, the affinity purified
antibodies were analyzed using ELISA and Western Blot analyses.Molar F/P
ratio = 5.7 |
| Western Blots |
Recommended dilution range 1:5000 - 1:10,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
FITC Conjugated Rabbit anti-VSV-g |
|
| Host |
Rabbit |
| Catalog Number |
RV V-45F |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS with 0.2% BSA |
| Preservative |
0.1% Sodium Azide |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with VSV-g conjugated with
KLH. After multiple immunizations in Freunds adjuvant, serum was
collected. The anti-VSV-g antibodies were immunoaffinity purified using
the peptide immobilized on a solid phase and conjugated with FITC |
| Specification |
Molar F/P ratio of anti-VSV-g conjugat is 3-6. |
| Western Blots |
1 ug/ml - 0.1 ug/ml |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-VSV-g |
|
| Host |
Rabbit |
| Catalog Number |
RV V-45P-Z |
| Quantity |
0.1 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with YTDIEMNRLGK (VSV-g
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. Antibodies were immunoaffinity purified
using the peptide immobilized on a solid phase and conjugated with HRP. |
| Specification |
Antibody specificity was verified by ELISA against
the peptide conjugated to BSA (YTDIEMNRLGK-BSA). Recommended working
dilution ¨C 1:5,000 ¨C 1:20,000 |
| Western Blots |
Recommended dilution range 1:1,000 ¨C 1:5,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
| Item |
HRP Conjugated Rabbit anti-VSV-g |
|
| Host |
Rabbit |
| Catalog Number |
RV V-45P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
50 mM HEPES pH 7.0, 0.1 M Sodium Chloride with 0.2%
BSA |
| Preservative |
0.01% Thimerosal |
| Storage |
4 C |
| Procedure |
Rabbits were immunized with YTDIEMNRLGK (VSV-g
epitope) conjugated with KLH. After multiple immunizations in Freunds
adjuvant, serum was collected. Antibodies were immunoaffinity purified
using the peptide immobilized on a solid phase and conjugated with HRP. |
| Specification |
Antibody specificity was verified by ELISA against
the peptide conjugated to BSA (YTDIEMNRLGK-BSA). Recommended working
dilution ¨C 1:5,000 ¨C 1:20,000 |
| Western Blots |
Recommended dilution range 1:1,000 ¨C 1:5,000 |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications |
|
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58 90 45 
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