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Antibodies for Veterinary Diagnostics Spec
| Item |
Monoclonal anti- Canine Distemper Virus |
|
| Host |
Mouse |
| Catalog Number |
MDST-40M |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH, 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
SpA purified from culture supernatant IgG 2B Kappa. |
| Specification |
Specific for CDV; does not react with CAV2, CCV. CPI
or CPV. By Western blot, in reducing gels, gives major bands at 76, 68,
54 and 32 KD. By ELISA, works as both a capture and a probe. Effective
by IFA and only produces cytoplasmic staining and intense staining of
inclusion bodies. Also has been used in immunohistochemistry (paraffin
sections). |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Feline Infectious
Peritonitis Virus / Corona |
|
| Host |
Mouse |
| Catalog Number |
MCOR-40M-CCV2-2 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C for short term, frozen for long term. Do not
multiply freeze-thaw |
| Procedure |
Immunization of mice with feline/canine/porcine and
ferret coronavirus then hybridomas formed, screened and cloned |
| Specification |
ImPurified by protein A affinity chromatography of
tissue culture fluid.Antibody is IgG1 Kappa. Effective as a probe in
ELISA using FIPV3-70 as the capture antibody. Reacts with CCV and FIPV
and Ferret corona virus. Negative against Canine Adenovirus, CDV, Canine
Parvovirus and Canine Parainfluenza Virus.By immunofluoresence, positive
against CCV and FIPV(type 1 and 2).By Western Blot gives weak specific
bands at 56, 46 and 40 kD in reducing gels with CCV as antigen
munization of mice with feline/canine/porcine and ferret coronavirus
then hybridomas formed, screened and cloned |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Feline Infectious
Peritonitis Virus / Corona |
|
| Host |
Mouse |
| Catalog Number |
MCOR-20M-FIPV4-42 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C for short term, frozen for long term. Do not
multiply freeze-thaw |
| Procedure |
Immunization of mice with feline/canine/porcine and
ferret coronavirus then hybridomas formed, screened and cloned |
| Specification |
Purified by protein A affinity chromatography of
tissue culture fluid.Antibody is IgG1 Kappa.Excellent as a probe in
ELISA. Reacts with CCV and FIPV virus. IT IS FIPV SEROTYPE 2 SPECIFIC
AND NEGATIVE FOR FIPV SEROTYPE 1. Negative against Canine Adenovirus,
CDV, Canine Parvovirus and Canine Parainfluenza Virus.By
immunofluoresence: positive against CCV and FIPV (serotype 2). |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Feline Leukemia Virus
p27 |
|
| Host |
Mouse |
| Catalog Number |
MFLV-20M-8D11 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C for short term or ¨C70 for long term. Do not
multiply freeze-thaw |
| Procedure |
Monoclonal antibody is prepared in tissue culture
and the antibody is purified using Protein A affinity chromatography |
| Specification |
The purified monoclonal antibody is of the IgG1
Kappa class and recognizes the p27 antigen (anti gag) and higher
molecular weight gag precursors containing p27. Recognizes all FeLV
isolates tested (>25). Gives good ELISA and Western Blot signals |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Feline Leukemia Virus
p27 |
|
| Host |
Mouse |
| Catalog Number |
MFLV-20M-CM1 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C for short term or -70 for long term. Do not
multiply freeze-thaw |
| Procedure |
Monoclonal Antibody is prepared in tissue culture
and the antibody is purified using Protein A affinity chromatography |
| Specification |
The purified monoclonal antibody is of the IgG 2B
Kappa class and recognizes the p27 antigen ( anti gag) and higher
molecular weight gag precursors containing p27. Recognizes all FeLV
isolates tested (>25). Gives good ELISA and Western Blot signals. It has
recently been shown to pair well with CM 1 in certain ELISA assays |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Feline Immunodeficiency
Virus |
|
| Host |
Mouse |
| Catalog Number |
MFIV-20M-SU1 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.05% Sodium Azide |
| Storage |
4 C |
| Procedure |
Mice were immunized with pure gp105/120 env ANTIGEN,
Hybridomas were prepared and clones selected specific for gp105/120 |
| Specification |
The antibody is prepared from tissue culture fluids
by Protein A chromatography. The antibody is of the IgG1 kappa type
Specific for gp 105/120 envelope. Antigen and reacts with Petaluma,
34TF10, Ppr and Glasgow 8. Negative against FLV, Feline Herpes type 1,
Feline Coronavirus and Feline Calicivirus.Excellent probe for ELISA,
shows FIV virus neutralization properties and works in FACS and IF
analyses. |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Affinity Purified Chicken anti-Canine
Heartworm
|
|
| Host |
Chicken |
| Catalog Number |
CHRT-65A |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
PBS, pH 7.2 |
| Preservative |
0.1% sodium azide |
| Storage |
4 C |
| Procedure |
Chickens were immunized with extracts from extracts
from both male and female heartworms. Anti-heartworm antibodies were
then purified off an antigen containing immunoabsorbant. |
| Specification |
anti-heartworm is suitable for lateral flow
applications. |
| Western Blots |
1:1,000-1:5,000 |
| ELISA |
coating 1:200-1:500 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
HRP Conjugated Chicken anti-Canine
Heartworm
|
|
| Host |
Chicken |
| Catalog Number |
CHRT-65P |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Preservative |
0.01% thimerosal |
| Storage |
4 C |
| Procedure |
Chickens were immunized with extracts from extracts
from both male and female heartworms. Antibodies were then purified off
an antigen containing immunoabsorbant and conjugated with horseradish
peroxidase (HRP). |
| Specification |
Suitable for ELISA and Western Blotting. |
| Western Blots |
1:1000-1:5000 |
| ELISA |
1:5,000 - 1:20,000 |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Canine Heartworm |
|
| Host |
Mouse |
| Catalog Number |
MHRT-40M-39 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Immunization of mice with crude male, female and
immature heartworm antigens isolated from dogs. The spleen and lymph
node lymphoctes were fused to form hybridomas |
| Specification |
The IgG antibodies from this hybridoma were isolated
from tissue culture fluids using precipitation, gel filtration and
Staph. Protein A purification procedures. This is an IgG 1 Kappa which
gives multiple bands by Western blot analysis consistent with both male
and female antigens. This also works well as capture antibody for ELISA
assays |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Canine Heartworm |
|
| Host |
Mouse |
| Catalog Number |
MHRT-40M-81 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline, pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Immunization of mice with crude male, female and
immature heartworm antigens isolated from dogs. The spleen and lymph
node lymphoctes were fused to form hybridomas |
| Specification |
The IgG antibodies from this hybridoma were isolated
from tissue culture fluids using precipitation, gel filtration and
Staph. Protein A purification procedures. This is an IgG 1 Kappa which
gives multiple bands by Western blot analysis consistent with both male
and female antigens. This clone gives a very strong 33 kD band
consistent with an anti-larval activity and works well as capture
antibody for ELISA assays |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
| Item |
Monoclonal anti-Canine Heartworm |
|
| Host |
Mouse |
| Catalog Number |
MHRT-40M-83 |
| Quantity |
1.0 mg |
| Physical State |
Liquid |
| Buffer |
Phosphate buffered saline pH 7.2 |
| Preservative |
0.05% sodium azide |
| Storage |
4 C |
| Procedure |
Immunization of mice with crude male, female and
immature heartworm antigens isolated from dogs. The spleen and lymph
node lymphoctes were fused to form hybridomas |
| Specification |
The IgG antibodies from this hybridoma were isolated
from tissue culture fluids using precipitation, gel filtration and
Staph. Protein A purification procedures. This is an IgG 1 Kappa which
gives multiple bands by Western blot analysis consistent with both male
and female antigens. It also works well as capture antibody for ELISA
assays |
| Western Blots |
N/A |
| ELISA |
N/A |
| Application |
For Research and Manufacturing Only; Not for Use in
Diagnostic Applications. |
|
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