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Chemical Product and Company Identification
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Name
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DNA Polymerase, TAQ Cat # 11-BIO-21-40
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Uses
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DNA
amplification via PCR |
Product features
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Highly
processive 5'–3' DNA Polymerase |
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Extremely thermostable |
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Recombinant enzyme for best reproducible results
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Hazards
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Health: 1
Flammability: 0
Reactivity: 0
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Company Identification
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GENTAUR LABORATORIES Avenue de l'Armée 68
1040 Brussels, BELGIUM
Tel: +32 2 732 56 88
Fax: + 32 2 732 44 14
e-mail: info@gentaur.com
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In case of Emergency
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Call : GENTAUR Tel : +32 (0) 2 732 56 88
Out of office: Centre Antipoisons (Brussels) Tel: +32 (0) 70 245 245
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Identity Information |
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CAS Registry Number |
9012-90-2 |
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EC (EINECS/ELINCS) |
232-741-2 |
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Physical/Chemical
Characteristics |
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Concentration |
5 Unit/ul, 500Units/vial |
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Included |
10X reaction buffer and 25mM MgCl2
buffer |
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Appearance |
White to brown coloured liquid |
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Odour |
Typical enzyme odour |
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Sources |
Thermus aquaticus |
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Error rate |
1/105 |
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Unit definition |
One unit incorporates 10 nmole of dNTP into
acid-insoluble material in 30 min. at 74°C. |
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QUALITY TESTING |
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Our Thermostable DNA Polymerase is highly
purified, free of contaminating endonucleases, exonucleases and
nicking activity. For endonuclease assay, 1 ug of Lambda/Hind
III DNA is incubated with 20 units of the enzyme in assay buffer
at 75°C for 16 hrs and no visible contaminating activity is
observed; For exonucleases assay, 1 ug of pBR322 plasmid DNA is
incubated with 10 units of enzyme for 16 hrs at 75°C in assay
buffer and no detectable exonuclease is observed. The purity of
the enzyme is also evaluated by adding 10 units of DNA
polymerase in 100 ul of a reaction mixture for making first
strand cDNA at beginning and no impaired effect on the first
strand is observed. |
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10X REACTION BUFFER(Without MgCl2) |
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500 mM KCl, 100 mM Tris HCl (pH 9.0) at 25°C, 1%
Triton X-100 Buffer is optimized for use with 200 uM dNTPs |
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25mM MgCl2 BUFFER |
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In general, 1.5-2.5 mM final concentration
of MgCl2 is recommended. |
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*The final MgCl2 concentration may
be variable according to individual experiment requirements. |
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STORAGE BUFFER |
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20 mM Tris HCl ( pH 8.0 ), 0.l mM EDTA, lm M DTT,
0.1% Triton X-100, 50% glycerol |
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EXTENSION CHARACTERISTIC |
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Our Thermostable DNA Polymerase has the
independent terminal transferal activity which results in the
addition of a single nucleotide ( adenosine ) at 3' end of the
extension product. TA cloning vector is recommended if the
extension product is needed to be cloned. |
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Storage |
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Storage
Temperature |
Store at -20°C and keep tightly closed. |
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Warning |
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For Research Use Only. Not for drug, household or
other uses. See Material Safety Data Sheet for additional
information. |
+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45