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Taq Plus DNA Polymerase
(Cat. No.: E009)
Description:
Taq
Plus DNA Polymerase is a special
formulation designed for amplify large
fragment. The main component is Taq DNA
Polymerase, and some special addictives
such as Pfu DNA Polymerase are added to
enhance the efficiency of amplification
reaction. Theoretically, Taq Plus
produces significantly higher yields of
PCR products than ordinary Taq
Polymerase, especially for fragments >1
kb, and can amplify up to 30 kb. Taq
Plus also contains a proofreading
activity that reduces the error rate of
Taq Polymerase. Taq Plus is suitable as
a direct replacement for ordinary Taq
Polymerase in most applications.
Features:
High fidelity:
with an error frequency of 1.6X10-6
during DNA synthesis.
Higher yield:
Taq Plus increases the efficiency of
polymerization reaction, resulting in a
great percentage of extenuation reaction
completion up to 10 kb to 30 kb.
Rely on a Performance-Tested Enzyme: SinoBio
PCR systems, enzymes and reagents are
proven in PCR to ensure reliable,
high-performance results. If you are not
completely satisfied with any of our PCR
product, we will send a replacement or
refund your account.
Applications:
Long PCR (up to 30 kb),
PCR cloning, RT-PCR etc.
Unit Definition:
One
unit of the enzyme catalyzes the
incorporation of 10nmole of
deoxyribonucleotides into a
polynucleotide fraction in 30min at 74oC.
Quality Control Tests:
PCR, Activity,
endonuclease/nickase, Specific
performance test.
Storage:
Taq Plus DNA polymerase in 20mM Tris-HCl,
pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT,
50% glycerol, 0.5% NP40 and 0.5% Tween
20 should be stored at -20oC.
10X Reaction Buffer with
MgCl2:
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25oC)
and 1% Triton X-100£¬
100mM (NH4)2SO4,
and 35mM MgCl2.
Buffer is optimized for use with 0.2mM
of each dNTP.
Reaction Condition:
DNA synthesis is
performed in 100µl of mixture containing
20-200µM dNTPs, 0.3-1µM Primers,
0.1-0.250mg of template DNA, 10 µl of 10
x reaction buffer and 2.5-5 units of Taq
Plus. Mix the reaction gently,
centrifuge briefly and then overlay with
light mineral oil.
Initially, denature the
reaction by incubating at 95oC
for 5 minutes and then cool to 40-68oC
for 5 minutes to allow the primers to
anneal to the template DNA. |
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