Taq DNA Polymerase
(Cat. No.: E001)
Taq DNA Polymerase is a thermostable
enzyme of approximately 94kDa isolated
from Thermus aquaticus. This unmodified
enzyme replicates DNA at 74oC
and exhibits a half-life of 40 minutes
at 95oC . The enzyme
catalyzes the polymerization of
nucleotides into duplex DNA in the 5´-3´
direction in the presence of magnesium
and also possesses a 5´-3´ exonuclease
activity. Taq DNA Polymerase is
recommended for use in PCR but is not
recommended for use in DNA sequencing
Thermus aquaticus YT1.
Depend on an Enzyme That Works:
Compositions of the storage buffers have
been optimized to assure quality
performance of the enzyme under a
variety of conditions.
Specify Your Own Reaction
Conditions: Choose either Taq
with Mg-free 10X Reaction Buffer and
separate 25mM MgCl2 or Taq
with 10X Reaction Buffer containing 15mM
Rely on a Performance-Tested
Enzyme: SinoBio PCR systems,
enzymes and reagents are proven in PCR
to ensure reliable, high-performance
results. If you are not completely
satisfied with any of our PCR product,
we will send a replacement or refund
PCR. 3´ A-tailing of blunt ends,
compatible with Vectors.
One unit is defined as the amount of
enzyme required to catalyze the
incorporation of 10nmol of dNTP into
acid-insoluble material in 30 minutes at
74oC. The reaction conditions
are: 50mM Tris-HCl (pH 9.0 at 25oC),
50mM NaCl, 5mM MgCl2, 200µM
each of dATP, dCTP, dGTP, dTTP (a mix of
unlabeled and [3H]dTTP), 10µg
activated calf thymus DNA and 0.1mg/ml
BSA in a final volume of 50µl.
Quality Control Tests:
PCR (activity), SDS-PAGE
Taq DNA polymerase in 20mM
Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA,
5mM DTT, 50% glycerol, 0.5% NP40 and
0.5% Tween 20 should be stored at -20oC.
10X Reaction Buffer:
10X Reaction Buffer with MgCl2:
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25oC),
1% Triton X-100 and 15mM MgCl2.
Buffer is optimized for use with 0.2mM
of each dNTP.
10X Reaction Buffer without
MgCl2: 500mM KCl, 100mM
Tris-HCl (pH 9.0 at 25oC) and
1% Triton X-100. include: 10X Reaction
Buffer without MgCl2 and
separate 25mM MgCl2 Solution.
Chien, A. et al. (1976) J.
Bacteriol. 127, 1550-7.
Kaledin, A.S. et al. (1980)
Biokhimiia 45, 644-51.
Some applications in which
this product can be used may be covered
by patents issued and applicable in the
United States and certain other
countries. Because purchase of this
product does not include a license to
perform any patented application, users
of this product may be required to
obtain a patent license depending upon
the particular application in which the
product is used. The PCR process is the
subject of European Patent Nos. 201,184
and 200,262 owned by Hoffman-LaRoche.
Those patents will expire on March 28,
2006. The corresponding PCR process
patents in the United States expired on
March 29, 2005.