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Taq DNA Polymerase datasheet

 

Taq DNA Polymerase
(Cat. No.: E001)

Description:
    Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74oC and exhibits a half-life of 40 minutes at 95oC . The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5-3 direction in the presence of magnesium and also possesses a 5-3 exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions. 

Source:
    Thermus aquaticus YT1.

Features:
    Depend on an Enzyme That Works:
Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions.
    Specify Your Own Reaction Conditions: Choose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl or Taq with 10X Reaction Buffer containing 15mM MgCl2 .
    Rely on a Performance-Tested Enzyme: SinoBio PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account.

Applications:
    PCR. 3 A-tailing of blunt ends, compatible with Vectors.

Unit Definition:
    One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74oC. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25oC), 50mM NaCl, 5mM MgCl2, 200M each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50l.

Quality Control Tests:
    PCR (activity), SDS-PAGE (purity), endonuclease/nickase.

Storage:
    Taq DNA polymerase in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol, 0.5% NP40 and 0.5% Tween 20 should be stored at -20oC.

10X Reaction Buffer:
    10X Reaction Buffer with MgCl2:
500mM KCl, 100mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP.
    10X Reaction Buffer without MgCl2: 500mM KCl, 100mM Tris-HCl (pH 9.0 at 25oC) and 1% Triton X-100.  include: 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution.

References:  
    Chien, A. et al. (1976) J. Bacteriol. 127, 1550-7.
    Kaledin, A.S. et al. (1980) Biokhimiia 45, 644-51.
 
Patents/Disclaimers:
    Some applications in which this product can be used may be covered by patents issued and applicable in the United States and certain other countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. The PCR process is the subject of European Patent Nos. 201,184 and 200,262 owned by Hoffman-LaRoche. Those patents will expire on March 28, 2006. The corresponding PCR process patents in the United States expired on March 29, 2005.
 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Arme 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Copyright 2005 Gentaur BVBA
Last modified: feb-07