Parathyroid Hormone (PTH1-84)
(Cat. No.: C012)
Parathyroid hormone is the
most important endocrine regulator of
calcium and phosphorus concentration in
extracellular fluid. This hormone is
secreted from cells of the parathyroid
glands and finds its major target cells
in bone and kidney. Another hormone,
parathyroid hormone-related protein,
binds to the same receptor as
parathyroid hormone and has major
effects on development.
Like most other protein hormones,
parathyroid hormone is synthesized as a
preprohormone. After intracellular
processing, the mature hormone is
packaged within the Golgi into secretory
vesicles, the secreted into blood by
exocytosis. Parathyroid hormone is
secreted as a linear protein of 84 amino
Recombinant Human PTH produced in E.
coli is a single, non-glycosylated,
polypeptide chain containing 84 amino
acids and having a molecular mass of 9
Activity: rHuPTH is fully
biologically active when compared to
standards. The activity calculated by
UMR106 cell/cAMP method corresponding to
a specific activity of 1.0 x 104
than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing
and non-reducing SDS-PAGE Silver Stained
than 0.1 ng/µg (IEU/µg) of rHuPTH.
protein (1mg/ml) was lyophilized after
extensive dialyses against 1.15mg sodium
citrate, sodium chloride 7.31mg, 0.21mg
citric acid, 0.1117 EDTA-Na2,
0.2mg Tween 80 and 50mgs Mannitol.
rHuPTH although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution rHuPTH should be
stored at 4oC between 2-7
days and for future use below -18oC.
For long-term storage it is recommended
to add a carrier protein (0.1% HSA or
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized rHuPTH in sterile 18M¦¸-cm H2O
not less than 100µg/ml, which can then
be further diluted to other aqueous