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Pfu DNA
Polymerase
(Cat. No.: E002)
Description:
Pfu DNA Polymerase is a thermostable
enzyme of approximately 90kDa isolated
from Pyrococcus furiosus. The enzyme
replicates DNA at 75oC,
catalyzing the polymerization of
nucleotides into duplex DNA in the 5´-3´
direction in the presence of magnesium.
Pfu DNA Polymerase also possesses 3´-5´
exonuclease (proofreading) activity.
Base misinsertions that may occur during
polymerization are rapidly excised by
the proofreading activity of the
polymerase. Consequently, Pfu DNA
Polymerase is recommended for use in PCR
and primer extension reactions that
require high-fidelity synthesis. Pfu DNA
Polymerase-generated PCR fragments are
blunt-ended.
Source:
Pyrococcus furiosus
strain Vc1 DSM3638.
Features:
High Fidelity: Pfu
DNA Polymerase exhibits the lowest error
rate of any thermostable DNA polymerase.
Performance Guarantee:
SinoBio PCR systems, enzymes and
reagents are proven in PCR to ensure
reliable, high-performance results. If
you are not completely satisfied with
any our PCR product, we will send a
replacement or refund your account.
Applications:
Pfu DNA Polymerase is recommended
for use in PCR, primer extension
reactions and other applications that
demand high fidelity.
Unit Definition:
One unit is defined as the amount of
enzyme required to catalyze the
incorporation of 10nmol of dNTPs into
acid-insoluble material in 30 minutes at
75oC.
Quality Control Tests:
PCR (activity), SDS-PAGE
(purity), endonuclease/nickase.
Storage:
Pfu DNA Polymerase in 50mM
Tris-HCl (pH 8.2 at 25oC),
0.1mM EDTA, 1mM DTT, 50% glycerol and
0.05% CHAPS should be stored at -20oC.
10X Reaction Buffer with MgSO4:
200mM Tris-HCl (pH 8.8 at 25oC),
100mM KCl, 100mM (NH4)2SO4,
20mM MgSO4, 1.0% Triton X-100
and 1mg/ml nuclease-free BSA.
References:
Fiala, G. and Stetter, K.O.
(1986) Arch. Microbiol. 145,
56.
Lundberg, K.S. et al. (1991)
Gene 108, 1-6.
Flaman, J.M. et al. (1994) Nucl.
Acids Res. 22, 3259-60.
Cline, J. et al. (1996) Nucl.
Acids Res. 24, 3546-51.
Andre, P. et al. (1997) Genome
Res. 7, 843-52.
Patents/Disclaimers:
Certain applications of
this product are covered by patents
issued to parties other than Promega and
applicable in certain countries. Because
purchase of this product does not
include a license to perform any of
these patented applications, users of
this product may be required to obtain a
patent license depending upon the
particular application and country in
which the product is used.
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