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Pfu DNA Polymerase datasheet

 

Pfu DNA Polymerase
(Cat. No.: E002)

Description:
    Pfu DNA Polymerase is a thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75oC, catalyzing the polymerization of nucleotides into duplex DNA in the 5´-3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´-5´ exonuclease (proofreading) activity. Base misinsertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Consequently, Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.

Source:
    Pyrococcus furiosus strain Vc1 DSM3638.

Features:
    High Fidelity: Pfu DNA Polymerase exhibits the lowest error rate of any thermostable DNA polymerase.
    Performance Guarantee: SinoBio PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any our PCR product, we will send a replacement or refund your account.
 
Applications:
    Pfu DNA Polymerase is recommended for use in PCR, primer extension reactions and other applications that demand high fidelity.

Unit Definition:
    One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 minutes at 75oC.

Quality Control Tests:
    PCR (activity), SDS-PAGE (purity), endonuclease/nickase.

Storage:
    Pfu DNA Polymerase in 50mM Tris-HCl (pH 8.2 at 25oC), 0.1mM EDTA, 1mM DTT, 50% glycerol and 0.05% CHAPS should be stored at -20oC.
 

10X Reaction Buffer with MgSO4:
    200mM Tris-HCl (pH 8.8 at 25oC), 100mM KCl, 100mM (NH4)2SO4, 20mM MgSO4, 1.0% Triton X-100 and 1mg/ml nuclease-free BSA.

References:
    Fiala, G. and Stetter, K.O. (1986) Arch. Microbiol. 145, 56.
    Lundberg, K.S. et al. (1991) Gene 108, 1-6.
    Flaman, J.M. et al. (1994) Nucl. Acids Res. 22, 3259-60.
    Cline, J. et al. (1996) Nucl. Acids Res. 24, 3546-51.
    Andre, P. et al. (1997) Genome Res. 7, 843-52.
 
Patents/Disclaimers:
    Certain applications of this product are covered by patents issued to parties other than Promega and applicable in certain countries. Because purchase of this product does not include a license to perform any of these patented applications, users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used. 
 

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07