Human Interferon-alpha 2b (PEG-IFNa2b)
(Cat. No.: C007)
At least 23 different variants of
Interferon-alpha are known. The
individual proteins have molecular
masses between 19-26 kDa and consist of
proteins with lengths of 156-166 and 172
amino acids. All IFN-alpha subtypes
possess a common conserved sequence
region between amino acid positions
115-151 while the amino-terminal ends
are variable. Many IFN-alpha subtypes
differ in their sequences at only one or
two positions. Naturally occurring
variants also include proteins truncated
by 10 amino acids at the
Recombinant Human Interferon
alpha-2b produced in E. coli is a
single, non-glycosylated, polypeptide
chain containing 165 amino acids and
having a molecular mass of 19269 Dalton.
Pegylated IFN-alpha 2b is manufactured
by attaching a 40KD NHS-mPEG to the
primary amino acid of IFN- 2b.
Pegylated IFN alpha-2b is fully
biologically active when compared to
standard. The specific activity as
determined in a viral resistance assay
using bovine kidney MDBK cells was found
to be 5 x 106 IU/mg.
than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing
and non-reducing SDS-PAGE Silver Stained
Amino acid sequence:
The sequence of the first five
N-terminal amino acids was determined
and was found to be
than 0.1 ng/µg (IEU/µg) of IFN-alpha
Lyophilized from a (1mg/ml) solution in
containing 5.55mg Sodium phosphate
dibasic, 5.55mg sodium phosphate
monobasic buffer, 296mg sucrose and
0.37mg tween 80.
PEG-IFN alpha-2b although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution PEG-IFN alpha-2b
should be stored at 4oC
between 2-7 days and for future use
below -18oC. For long-term
storage it is recommended to add a
carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized PEG-IFN alpha-2b in sterile
18M¦¸-cm H2O not less than
100µg/ml, which can then be further
diluted to other aqueous solutions.