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PEG-IFNα2b datasheet

 

Pegylated Recombinant Human Interferon-alpha 2b (PEG-IFNa2b)
(Cat. No.: C007)

Background: 
    At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Description:
    Recombinant Human Interferon alpha-2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19269 Dalton. Pegylated IFN-alpha 2b is manufactured by attaching a 40KD NHS-mPEG to the primary amino acid of IFN- 2b.

Quality Control:
    Biological Activity:  Pegylated IFN alpha-2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5 x 106 IU/mg.
    Purity: Greater than 98.0% as determined by:
              (a) Analysis by RP-HPLC.
              (b) Anion-exchange FPLC.
              (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
    Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asp-Leu-Pro-Gln.
    Endotoxin: Less than 0.1 ng/g (IEU/g) of IFN-alpha 2b. 

Formulation: Lyophilized from a (1mg/ml) solution in containing 5.55mg Sodium phosphate dibasic, 5.55mg sodium phosphate monobasic buffer, 296mg sucrose and 0.37mg tween 80. 

Storage: Lyophilized PEG-IFN alpha-2b although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution PEG-IFN alpha-2b should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles.

Reconstitution: It is recommended to reconstitute the lyophilized PEG-IFN alpha-2b in sterile 18M-cm H2O not less than 100g/ml, which can then be further diluted to other aqueous solutions.

 

 

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Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Copyright 2005 Gentaur BVBA
Last modified: feb-07