(Cat. No.: C011)
Cytokines of the
IL6/GCSF/MGF family are glycoproteins of
about 170 to 180 amino acid residues
that contain four conserved cysteine
residues involved in two disulphide
bonds. They have a compact, globular
fold (similar to other interleukins),
stabilized by the 2 disulphide bonds.
One half of the structure is dominated
by a 4 alpha-helix bundle with a
left-handed twist: the helices are
anti-parallel, with 2 overhand
connections, which fall into a
2-stranded anti-parallel beta-sheet. The
fourth alpha helix is important to the
biological activity of the molecule.
Interleukin (IL)-6 is an important
proinflammatory and immunoregulatory
cytokine expressed by various cells.
Interleukin-6 has been shown to inhibit
the growth of early stage and to promote
the proliferation of advanced stage
melanoma cells in vitro.
Recombinant Human IL-6 produced in
E. coli is a single, non-glycosylated
polypeptide chain containing 184 amino
acids and having a molecular mass of
activity: rHuIL-6 is fully
biologically active when compared to
standard. The ED50 as determined by the
dose-dependant stimulation of murine
7TD1 cells is less then 0.1 ng/ml,
corresponding to a Specific Activity of
5 x 107 IU/mg.
than 98.0% as determined by:
(a) Analysis by
(c) Analysis by
reducing and non-reducing SDS-PAGE
Silver Stained gel.
21 KD+/-10% determined by reduced
The sequence of the first five
N-terminal amino acids was determined
and was found to be Ser-Pro-Val-Pro-Pro.
than 0.3ng/µg (0.3IEU/µg) determined by
Lyophilized from a concentrated (1mg/ml)
solution in water containing 43µg/ml
sodium chloride and 50µg/ml HSA
rHuIL-6 although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution rHuIL-6 should be
stored at 4oC between 2-7
days and for future use below -18oC.
For long-term storage it is recommended
to add a carrier protein (0.1% HSA or
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized rHuIL-6 in sterile 18M¦¸-cm
H2O not less than 100µg/ml,
which can then be further diluted to
other aqueous solutions.