Home Feedback Search Online Order CATALOG

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

Back Up Next


 

 

IL-17 datasheet

 

Recombinant Human Interleukin-17 (IL17)
(Cat. No.: C021)

Background:
    IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice.
    As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation. 

Description:
    Recombinant Human IL-17 produced in E. coli is a single, non-glycosylated polypeptide chain containing 137 amino acids and having a molecular mass of 15668 Dalton.

Quality Control:
    Biological Activity: The ED50 as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts was found to be approximately 2ng/ml, corresponding to a Specific Activity of 5 x 105 IU/mg. 
    Purity: Greater than 98.0% as determined by:
               (a) Analysis by RP-HPLC.
               (b) Anion-exchange FPLC.
               (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 
    Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Val-Lys-Ala.
    Endotoxin: Less than 0.1ng/g (IEU/g) of rHuIL-17.

Formulation: Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Storage: Lyophilized rHuIL-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuIL-17 should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles. 

Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-17 in sterile 18M-cm H2O not less than 100g/ml, which can then be further diluted to other aqueous solutions.

 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Arme 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

Send mail to webmaster with questions or comments about this web site.
Copyright 2005 Gentaur BVBA
Last modified: feb-07