(Cat. No.: C021)
IL-17 is a potent proinflammatory
cytokine produced by activated memory T
cells. There are at least six members of
the IL-17 family in humans and in mice.
As interleukin-17 shares properties
with IL-1 and TNF-alpha, it may induce
joint inflammation and bone and
cartilage destruction. This cytokine is
found in synovial fluids of patients
with rheumatoid arthritis, and produced
by rheumatoid arthritis synovium. It
increases IL-6 production, induces
collagen degradation and decreases
collagen synthesis by synovium and
cartilage and proteoglycan synthesis in
cartilage. Interleukin-17 is also able
to increase bone destruction and reduce
its formation. Blocking of
interleukin-17 with specific inhibitors
provides a protective inhibition of
cartilage and bone degradation.
Recombinant Human IL-17
produced in E. coli is a single,
non-glycosylated polypeptide chain
containing 137 amino acids and having a
molecular mass of 15668 Dalton.
Activity: The ED50 as
determined by the dose-dependent
induction of IL-6 in primary human
foreskin fibroblasts was found to be
approximately 2ng/ml, corresponding to a
Specific Activity of 5 x 105
than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing
and non-reducing SDS-PAGE Silver Stained
The sequence of the first five
N-terminal amino acids was determined
and was found to be Met-Ile-Val-Lys-Ala.
than 0.1ng/µg (IEU/µg) of rHuIL-17.
Lyophilized from a concentrated (1mg/ml)
solution containing no additives.
rHuIL-17 although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution rHuIL-17 should be
stored at 4oC between 2-7
days and for future use below -18oC.
For long-term storage it is recommended
to add a carrier protein (0.1% HSA or
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized rHuIL-17 in sterile 18M¦¸-cm
H2O not less than 100µg/ml,
which can then be further diluted to
other aqueous solutions.