IL-17 datasheet

Recombinant Human Interleukin-17 (IL17)
(Cat. No.: C021)

Background:
    IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice.
    As interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation. 

Description:
    Recombinant Human IL-17 produced in E. coli is a single, non-glycosylated polypeptide chain containing 137 amino acids and having a molecular mass of 15668 Dalton.

Quality Control:
    Biological Activity: The ED50 as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts was found to be approximately 2ng/ml, corresponding to a Specific Activity of 5 x 10⁵ IU/mg. 
    Purity: Greater than 98.0% as determined by:
               (a) Analysis by RP-HPLC.
               (b) Anion-exchange FPLC.
               (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. 
    Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ile-Val-Lys-Ala.
    Endotoxin: Less than 0.1ng/µg (IEU/µg) of rHuIL-17.

Formulation: Lyophilized from a concentrated (1mg/ml) solution containing no additives.

Storage: Lyophilized rHuIL-17 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-17 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles. 

Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-17 in sterile 18M¦¸-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.