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HSA-IFN datasheet

 

Recombinant HAS-Interferon-alpha 2b (HSA-IFNa2b)
(Cat. No.: C004)

Background: 
    At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Description:
    Recombinant HSA fousion Interferon alpha-2b produced in P. Pichia is a single, non-glycosylated, and polypeptide chain having a molecular mass of 85.7KD.

Quality Control:
    Biological Activity:  HSA-IFNa2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 3 x 106 IU/mg.
    Purity: Greater than 98.0% as determined by: 
                 (a) Analysis by RP-HPLC.
                 (b) Anion-exchange FPLC.
                 (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
    Endotoxin: Less than 0.1 ng/g (IEU/g) of HSA-IFN-alpha 2b.  

Formulation: Lyophilized from a (1mg/ml) solution in containing 5.55mg Sodium phosphate dibasic, 5.55mg sodium phosphate monobasic buffer, 296mg sucrose and 0.37mg tween 80.  

Storage: Lyophilized HSA-IFN alpha-2b although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution HAS-IFN alpha-2b should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles.

Reconstitution: It is recommended to reconstitute the lyophilized HAS-IFN alpha-2b in sterile 18M-cm H2O not less than 100g/ml, which can then be further diluted to other aqueous solutions.


 

 

 

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International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033

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Last modified: feb-07