(Cat. No.: C001)
Erythropoietin is a member
of the hematopoietic growth factor
family and is one of the few
hematopoietic growth factors which
behaves like a hormone. The principal
function of erythropoietin is to couple
oxygen delivery by circulating red cells
to long-term tissue oxygen needs.
Produced primarily in the kidneys and to
a small extent in the liver in adults,
erythropoietin interacts in the bone
marrow with specific receptors on the
surface of erythroid progenitor cells to
initiate their entry into cell cycle if
dormant or to maintain their viability
while differentiating, if they are
already in active cell cycle.
Erythropoietin achieves its effects by
causing homodimerization of its receptor
with the resultant autophosphorylation
of the tyrosine kinase JAK2 and
phosphorylation of the receptor itself,
as well as various substrate proteins
leading to the upregulation of a number
of signaling pathways and the activation
of gene transcription.
Erythropoietin-alpha is produced in
Chinese hamster ovary (CHO) cells by
recombinant DNA technology is a single,
polypeptide chain containing 165 amino
acids and having a predicted molecular
mass of 21,000 Dalton and apparent
glycosylated molecular mass of 30,400
The specific activity was 1.2 x 105
than 98% as determined by
(a) Analysis by SEC-HPLC.
(b) Analysis by reducing
and non-reducing SDS-PAGE Silver Stained
the main zone between 3.3~4.3 analysis
The sequence of the first fifteen
N-terminal amino acids was determined
and was found to be
than 0.02ng/µg (0.02IEU/µg) determined
by LAL test.
Formulation: Each mg
of lyophlilzed powder contains 5.8mg
sodium citrate, 5.8mg sodium chloride
and 0.06mg citric acid.
rHuEPO-alpha although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution rHuEPO-alpha should
be stored at 4oC between 2-7
days and for future use below -18oC.
For long-term storage it is recommended
to add a carrier protein (0.1% HSA or
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized rHuEPO-alpha in sterile
18M¦¸-cm H2O not less than
100µg/ml, which can then be further
diluted to other aqueous solutions.