Recombinant Human soluble
CD40 (CD154) Ligand/TRAP
(Cat. No.: C014)
CD40L or CD154 is a membrane
glycoprotein and differentiation antigen
expressed on the surface of T-cells. The
CD40 ligand stimulates B-cell
proliferation and secretion of all
immunoglobulin isotypes in the presence
of cytokines. CD40 ligand has been shown
to induce cytokine production and
tumoricidal activity in peripheral blood
monocytes. It also costimulates
proliferation of activated T-cells and
this is accompanied by the production of
IFN-gamma, TNF-alpha, and IL2.
Recombinant Human CD40L
produced in E. coli is a
non-glycosylated, Polypeptide chain
containing 149 amino acids and having a
molecular mass of 16308 Dalton.
The ED50 as determined by the
dose-dependant stimulation of IL-12 &
IL-8 induction by PMB (Peripheral
Mononuclear) cells was found to be 5-10
ng/ml, corresponding to a Specific
Activity of 1 x 105 IU/mg.
than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing
and non-reducing SDS-PAGE Silver Stained
Amino acid sequence:
The sequence of the first five
N-terminal amino acids was determined
and was found to be Met-Gln-Lys-Gly-Asp.
than 0.1 ng/µg (IEU/µg) of soluble
is lyophilized from a sterile
concentrated solution (1mg/ml) with 10mM
Sodium Phosphate pH=7.5.
CD40L although stable at room
temperature for 3 weeks, should be
stored desiccated below -18oC.
Upon reconstitution CD40L should be
stored at 4oC between 2-7
days and for future use below -18oC.
For long-term storage it is recommended
to add a carrier protein (0.1% HSA or
Please avoid freeze-thaw
is recommended to reconstitute the
lyophilized CD40L in sterile 18M¦¸-cm H2O
not less than 100µg/ml, which can then
be further diluted to other aqueous