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Low cell binding plates/dishes HydroCell
Low cell binding plates/dishes
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Key features of HydroCell®
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♦Super hydrophilic polymer is fixed to the
surface of the dish/plate at nano-thickness level.
♦The surface is highly cells/protein resistent
♦Applications
Improved macrophage culture "in vitro".
Embryoid body formation from ES cells.
Spheroid formation in culture.
Preservation of scarce or rare samples.
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Advantages of HydroCell®
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Collection rate of cells/proteins
99%+
♦Reliable for collection of scarce samples
♦Resist attachment of very sticky cells
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INSTRUCTION
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Culturing cells
♦Do not scratch the surface of the dish/plates when
disseminating cells or changing media.
♦Otherwise same level of caution as non-polymer coated dish/plate
applies.
After collecting cells
♦The product is intended for one time use only. Please do not
re-use the product.
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96multi-well plate |
12multi-well plate |
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Wells/Bottom shape |
96(6f)/Flat |
12(23f)/Flat |
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Size |
86mm(L) X 128mm(W) |
86mm(L) X 128mm(W) |
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Area per well |
0.32 cm2 |
3.8 cm2 |
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Capacity per well |
0.3 ml |
7.0 ml |
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Material |
Polystyrene (with lid) |
Polystyrene (with lid) |
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Product No. |
CS2001-EX |
CS2002-EX |
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10cm Dish |
6cm Dish |
3.5cm Dish |
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Size |
100mm(D) X 20mm(H) |
60mm(D) X 15mm(H) |
35mm(D) X 10mm(H) |
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Area |
58.95 cm2 |
21.3 cm2 |
11.78 cm2 |
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Material |
Polystyrene (with lid) |
Polystyrene (with lid) |
Polystyrene (with lid) |
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Product No. |
CS2003-EX |
CS2004-EX |
CS2005-EX |
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Low cell binding plates/dishes
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Microscopic view of cells
cultured by "HydroCell®"
From the data below, it can be concluded that...
1) The collection rate of those dishes without polymer is very
poor.
2) There seems to be a large quality variance depending on the
kinds of the cells cultured among those dishes who also
advocates "low cell binding dishes".
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Macrophage were cultured on 3 different dishes holding
other variables constant. Attached cells were observed
on dish 2) and 3), whereas more than 99% of cells were
still floating for dish 1). |
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1) HydroCell® |
2) Other low cell-binding brand |
3) Non-coated |
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A-549 were cultured on 2 different dishes holding other
variables constant. Almost all the cells attached to the
surface of dish for 2). On the other hand, more than 99%
of cells were still floating for dish 1). |
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1) HydroCell® |
2) Non-coated |
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Background: Anchorage-independent cell growth is one of
the expressed feature of the cancer cells. By taking
this advantage, much easier and much higher-throughput
anti-cancer drug screening is possible by using
HydroCell. |
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Methodology: By taking normal NRK cells as a control (1.
EGF-·TGF-,2. EGF-·TGF+ ,3. EGF+·TGF-), NRK cells,
treated by EGF and TGF to derive cancer cells, were
cultured on normal 96 multi-well plate and 96 multi-well
HydroCell for 3 days. Their growth measured by comparing
the absorbance. |
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Summary of the findings: Anchorage-independent cell
growth, which is the primary feature of the cancer cells,
can be measured purely when HydroCell is used, without
the effect of external factors to the mono-layered cells.
Therefore much easier and much faster screening of
anti-cancer drug ,which specificly impede the cell
growth, is possible when HydroCell is used. |
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Microscopic view of NRK
cells(Day 3) (Normal culturing plate) |
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1)EGF-, TGF- |
2)EGF-, TGF+ |
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3)EGF+, TGF- |
4)EGF+, TGF+ |
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Microscopic view of NRK cells(Day
3) (HydroCell) |
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1)EGF-, TGF- |
2)EGF-, TGF+ |
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3)EGF+, TGF- |
4)EGF+, TGF+ |
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NRK Cell growth
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Low cell binding plates/dishes
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Q. |
Isn't it expensive when
compared to the other brands asserting same "low cellbinding"
dishes/plates? |
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A. |
There are other brands who are communicating similar concepts to
ours. However, depending on the type of the cells cultured, some
completely adhere to the surface of the dish or the corner of
the dishes for those brands. Ours is the sole product which even
the macrophage does not stick and achieves more than 99% of
collection rate irrespective of cell type or protein type
cultured. |
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Q. |
What are the other uses than
culturing immune cells such as macrophage? |
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A. |
We recommend such uses as...
1) Formation of embryoid ES cells
2) Anchorage independent coony formation of cancer cells
3) Spheroid formation |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
