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3D Culture Kit for human skin models ADM Scaffold ADMATRIX
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Product Description and
Application |
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ADMATRIX® enables you to compose human skin models
easily and handily.
ADMATRIX® is a highly bioactive ADM (Acellular Dermal
Matrix) made from tissue of normal porcine skin, which has the
most similar structure to that of human skin.
Fig.1 Porcine skin tissue- pre/post
acellularization (×100)
ADMATRIX® retains the basement membrane, with which
higher attachment rate of epithelial cells is shown. Bearing the
basement membrane, the structure of ADMATRIX® is very
close to the biological matrix, and which allows you to conduct
an in-vitro assay under a condition close to an invasion assay.
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Fig.2 Type IV collagen,
stained(×200) |
A package of this product contains the following items.
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ADMATRIX® |
6 packets (f22mm, 1 piece/packet) |
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Culture inserts |
6 pieces
(0. 8µm pore size,for 6-well plate) |
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Filter paper set |
1 set (f22mm, 10 sheets) |
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In a packet, ADMATRIX® is placed inside the culture
inserts. All the items contained in a package are sterilized and
individually wrapped.
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i) |
The operating procedure introduced in the next section
is an optimised process to compose human skin models.
Results can be varied depending on actual cells and
conditions adopted: Especially, culture medium, length
of incubation, or density of cell seeding are the
influential factors. Please optimise the conditions
according to the cells and experimental systems of
yours.
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ii) |
Due to its in-vivo like structure, ADMATRIX®
has a strong contractive tendency. This may cause a
product become slightly smaller or greater than its
designated size (F22mm, 0.6mm thick)
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iii) |
It is hard to determine the front side from the back of
ADMATRIX®. Please handle with care and
attention.
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iv) |
Please store ADMATRIX® in the original
package at 4°C. DO NOT turn over the package or
contents.
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v) |
All the procedures must be done under aseptic
conditions. |
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1. |
Preparation of ADMATRIX® |
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(1) |
Take out the packets of necessary quantity from the
storage kept at 4°C, and warm to room temperature. |
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(2) |
Open the packet in sterilized environment. Take out the
internal container from the packet and open the top lid. |
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(3) |
Use sterilized forceps. Check the ADM is placed between
sheets of filter paper in the culture insert. Place the
culture insert containing ADM in a 6-well plate. ADM is
originally set with the epidermal side up and the dermal
side down in the culture insert. DO NOT unnecessarily
turn ADM over. Once confused, it is hard to determine
its front side from the back. |
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(4) |
Culturing epithelial cells only: remove only the top
filter paper from ADM. The epidermal side (front side)
of ADM with the basement membrane is to work as
culturing base. |
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(5) |
Mesenchyme cells only or culturing on both sides: With
filter papers kept attached on both sides, turn the ADM
upside down in culture insert. |
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(6) |
After turning the ADM over, remove the filter paper on
top to reveal the dermal side for cell culturing. When
turning the ADM back over again, use supplied filter
paper as a backing. |
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2. |
How to make human skin models |
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Three-dimensional cultured human skin equivalent with
multi-layered epidermis can be composed with ADM used as
its scaffold. >>See reference 2)- 4). By using the both
sides of ADM for culturing, multiple kinds of cells can
be incorporated.
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(1) |
Seed the keratinocyte suspension of target cell number
onto the ADM surface (the basement membrane side) in
culture insert. Use growth medium for keratinocyte and
incubate at 37°C and 5% of CO2. |
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(2) |
When cells become confluent*, adjust the concentration
of Ca in the medium at over 1.6mM. This induces
differentiation of keratinocytes. (*Duration of
incubation varies depending on concentration of cell
suspension used) |
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(3) |
Expose epidermal surface to the air, and continue with
air-liquid interface culture for another couple of days.
This induces keratinocytes to become multi-layered and
form the corneum layer. |
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Skin model sample (epidermis) composed with ADMATRIX®
Fig. 3 Human keratinocytes (x
200) |
Where stored at 4°C, ADMATRIX® will keep its
stability for 6 months after the time of distribution.
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1) |
Y. Takami et al. Burns 22:182-190(1996) |
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2) |
K. H. Chakrabarty et al. Br J Dermatol 141:811-823
(1999) |
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3) |
D. R. Ralston et al. Br J Dermatol 140:605-615 (1999) |
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4) |
T. Qian et al. Br J Plastic Surgery 56:260-265(2003) |
CAUTIONS FOR STORAGE AND HANDLING
This product is for RESEARCH USE ONLY. DO NOT use this
for any other purpose, such as for human consumption,
cosmetic or domestic use.
Store this product at 4°C.
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Product code: |
B19-KRN-001 |
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Product license: |
PATPEND2003-430492,
PATPEND2004-24351 |
ADMATRIX is a trademark of BCS, Inc. |
 Brochures
for ADMATRIX™(309KB)
Application
Note I :Protocol for composing human skin models(215KB)
Application
Note II :Preservation stability data sheet (150KB)
Application
Note III :Safety data sheet(164KB)
Application
Note IV :Introduction to the VAS(Various Acellular Systems)
(216KB) |
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45