Tie2

Tie2 (human, recombinant, N-terminal His tag)
type : enzyme
pack size : 10 ug

Product: Tie2 (human, recombinant, N-terminal His tag)

Description: Human Tie2 GenBank Accession No. NM_000459), (a.a. 771-end) with N-terminal His tag, MW=45 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Stability: >6 months at –80°C

Purity: >80% by SDS-PAGE .

Specific Activity:140 U/ug. One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substarte per minute at pH 7.4 and 30 °C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug/ml recombinant Tie2.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference:
1. Ramsden,J.D., etal., Endocrinology 146 (7), 2895-2902 (2005)
2. Tammela,T., etal., Blood 105 (12), 4642-4648 (2005)