SHP-2 (224-529)/PTPN11

SHP-2
type : enzyme
pack size : 20 ug

Product Data: SHP-2 protein tyrosine phosphatase, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. NM_002834), (a.a. 224-529)with N-terminal GST tag, MW=59.5 kDa, expressed in an E.coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 3.8 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 5 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

References:
1. B.G. Neel et al. Trends Biochem. Sci. 2003 28 284
2. J.V. Ravetch & L.L. Lanier Science 2000 290 84
3. S. Yamazaki et al. J. Infect. Dis. 2003 187 334