RPTP r (801-1147)

RPTP r
type : enzyme
pack size : 20 ug

Product Data: RPTPgamma protein tyrosine phosphatase, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. NM_002841), (a.a. 801-1147)with N-terminal GST tag, MW=65.6 kDa, expressed in an E. coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 10 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 3 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1. C. Van Niekerk and L. Poels Cancer Lett. 1999, 137: 61
2. S. Liu, et al., Anticancer Res. 2002, 22:3917