PYK2

PYK2
type : enzyme
pack size : 10 ug

Product Data: PYK2 (human, recombinant, N-terminal His tag)

Description: Human PYK2 ( GenBank Accession No. U33284), full length with N-terminal His tag, MW=120 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Stability: >6 months at –80 °C

Purity: >90% by SDS-PAGE.

Specific Activity: 20 U/mg. One unit is defined as the amount of enzyme that will incorporate 1 nmol phosphate to the tyrosine substrate at pH 7.4 and 30 °C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant PYK2

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference:
1. Lev,S., etal., Nature 376 (6543), 737-745 (1995).