PTPmu (839-1100)

PTPmu
type : enzyme
pack size : 20 ug

Product Data: RPTPmu protein tyrosine phosphatase mu, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. BC051651), (a.a. 839-1100) with N-terminal GST tag, MW=56.2 kDa, expressed in an E. coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 680 U/ug. One unit will hydrolyze 1 pmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 10 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1. S. Brady-Kalnay, et al., J. Cell Biol 1995, 130: 977
2. T. Mourton, et al., J. Biol. Chem. 2002, 276: 14896