PTPbeta (1675-1996)

PTPbeta
type : enzyme
pack size : 20 ug

Product Data: RPTPbeta protein tyrosine phosphatase, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. X54131), (a.a. 1675-1996) with N-terminal GST tag, MW=63.7 kDa, expressed in an E. coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 40 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 0.5 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1. I. K. Lund et al., J Biol Chem. 2004, 279: 24226-35.
2. G.H. Peters et al., Biochimie. 2003, 85: 527-34.