PTP1B(1-321) rat

PTP1B
type : enzyme
pack size : 20 ug

Product Data: PTP1B protein tyrosine phosphatase, catalytic domain (rat)

Description: Catalytic domain, (a.a. 1-321) with N-terminal GST tag, MW=63.3 kDa, expressed in an E.coli expression system

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 20 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 2 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

References:
1. Frangioni et al., Cell, 1992, 68: 545
2. M. Elchebly et al., Science 1999, 283: 1544