PRL-1 (2-173)

PRL-1
type : enzyme
pack size : 10 ug

Product Data: PRL-1 protein tyrosine phosphatase, catalytic domain (human, recombinant,) Phosphatase of regenerating liver-1

Description: Catalytic domain, (Gene bank accession No. BC023975), (a.a. 1-173)with N-terminal GST tag, MW=45.7 kDa, expressed in an E.coli expression system.

Source: E.coli

Formulated in: 25 Mm Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50%
glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methyl
umbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30°C

Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 uM DiFMUP, and 50 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

References:
1. Q. Zeng et al., Cancer Res. 2003, 63: 2716-2722.
2. J. Wang et al., J. Biol. Chem 2002, 277: 46659-46668