PEST(2-300)/PTPN12

PTP-PEST
type : enzyme
pack size : 20 ug

Product Data: PTP-PEST protein tyrosine phosphatase, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. NM_002835), (a.a. 2-300)with N-terminal GST tag, MW=61.4 kDa, expressed in an E.coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 1 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 10 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates.

References:
1. J.F. Cote, et al., J Biol Chem. 2002, 277: 2973-86.
2. K. Horsch et al., Mol Endocrinol. 2001, 12: 2182-96