NEK6

NEK 6
type : enzyme
pack size : 10 ug

Product Name: NEK6 full length (human, recombinant, N-terminal His tag)

Description: Human NEK6 (GenBank Accession No. NM_014397)), (a.a. 1-313) with N-terminal HIS6 tag, MW=40.1 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Stability: >6 months at –80°C

Purity: >90% by SDS-PAGE.

Specific Activity: 50 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 7 peptide substrate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM NEK6 substrate, and 0.4 ug/ml NEK6.

Application: useful for the study of enzyme kinetics, screening inhibitors,and selectivity profiling.

Reference:
1. Yin, M.J. etal., J. Biol. Chem. 278 (52), 52454-52460 (2003).
2. Belham, C. etal., J. Biol. Chem. 278 (37), 34897-34909 (2003).