MEG-2 (285-593)/PTPN9

MEG2
type : enzyme
pack size : 20 ug

Product Data: MEG2 protein tyrosine phosphatase (PTP-MEG2), catalytic domain (human, recombinant, C-terminal His tag)

Description: Catalytic domain, (Gene bank accession No. M83738), (a.a. 285-593) with N-terminal GST tag, MW=60.3 kDa, expressed in an E.coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 2 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 10 ug/ml.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1. Q. Ying, et al., J. Cell Biochem. 2002, 86: 79
2. H. Huynh, et al. J. Immunol. 2003, 171: 6661