LAR (1282-1620)

LAR
type : enzyme
pack size : 20 ug

Product Data: LAR human transmembrane leukocyte antigen related PTP, catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. NM_130440), (a.a. 1330-1620) with N-terminal GST tag, MW=58 kDa, expressed in an E.coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Stability: 6 months at –80 °C

Purity: >95% by SDS-PAGE.

Specific Activity: 1 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C.

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 10 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates.

References:
1: H. Cho et al. Biochemistry 1991 30 6210
2: H. Cho et al. Biochemistry 1992 31 133
3: H. Cho et al. Prot. Sci. 1993 2 977