KDR/VEGFR2

VEGFR2/KDR
type : enzyme
pack size : 10 ug

Product Data: VEGFR2/KDR (human, recombinant, N-terminal GST tag)

Description: Human VEGFR2 GenBank Accession No. AF035121), (a.a. 805-1356) with N-terminal GST tag, MW=87 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.

Stability: >6 months at –80 °C

Purity: >90% by SDS-PAGE.

Specific Activity: 56 U/ug. One unit is defined as the amount of enzyme that will incorporate 1 nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30 °C

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant VEGFR2.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference:
1.Meyer, M. etal., EMBO J. 18, 363-374 (1999).
2. Rahimi, N. etal., J. Biol Chem 275, 16986-16992 (2000).