GSK3 alpha

GSK3alpha
type : enzyme
pack size : 10 ug

Product Data: GSK3alpha (human, recombinant, N-terminal GST tag)

Description: Human GSK3alpha GenBank Accession No. BC027984), full length, with N-terminal GST tag, MW=81.5 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM reduced glutathione, and 3 mM DTT.

Stability: >6 months at –80°C

Purity: >80% by SDS-PAGE.

Specific Activity: 700 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of glycogen synthase peptide 2 substrate per minute at pH 7.4 and 30°C

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 20 uM glycogen synthase peptide 2 substrate, and 2 ug/ml recombinant GSK3alpha.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference:
1. Meijer, L. etal., Trends Pharmacol Sci. 25 (9), 471-480 (2004).
2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).