Flt3

Flt3
type : enzyme
pack size : 10 ug

Product Data: Flt3 (human, recombinant, N-terminal His tag)

Description: Human Flt3 ( GenBank Accession No. NM_004119), (a.a. 564-end) with N-terminal His tag, MW=54.2 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Stability: >6 months at –80°C

Purity: >80% by SDS-PAGE.

Specific Activity: 20 U/mg. One unit is defined as the amount of enzyme that will incorporate 1 nmol phosphate to the tyrosine substrate per minute at pH 7.4 and 30°C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant Flt3.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Reference:
1. Takahashi,S., etal., Leuk. Res. 29 (8), 893-899 (2005).
2. Levis,M., etal., Blood 106 (2), 673-680 (2005).