DEP1 (1024-1306)

Dep-1
type : enzyme
pack size : 20 ug

Product Data:  DEP-1 protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. U10886), (a.a. 1024-1306) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli _expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 3 mM DTT.

Stability: 6 months at –80°C

Purity: >95% by SDS-PAGE.

Specific Activity: 114 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 0.5 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12.
2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.