CD45 (584-1256)

CD45
type : enzyme
pack size : 10 ug

Product Data: CD45 protein tyrosine phosphatase, catalytic domain (human, recombinant, N-terminal His tag)

Description: Catalytic domain, (Gene bank accession No. NM_080922), (a.a. 584-1256) with N-terminal His tag, MW=84.5 kDa, expressed in a Sf9 baculovirus expression system.

Source: Sf9 insect cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 5 mM DTT.

Stability: >6 months at –80 °C

Purity: >90% by SDS-PAGE.

Specific Activity: 25 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 2 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

References:
1. Rachmilewitz, J. et al., J. Biol. Chem. 278, 14059-14065
2. Fernandis, A.Z. et al., J. Biol. Chem. 278, 9536-9543.