Bas(2091-2490)/PTPL1/FAP-1/PTP N13

PTP-Bas
type : enzyme
pack size : 20 ug

Product Data:  PTP-Bas, (PTPL1/ FAP-1/ PTPN13) catalytic domain (human, recombinant)

Description: Catalytic domain, (Gene bank accession No. NM_080685), (a.a. 2091-2490) with N-terminal GST tag, MW=70.3 kDa, expressed in an E. coli expression system.

Source: E.coli

Formulated in: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.

Stability: 12 months at –80 °C

Purity: >95% by SDS-PAGE.

Specific Activity: 5 U/ug. One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30 °C

Assay buffer: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP and 10 ug/ml enzyme.

Application: useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

References:
1.W. Kimber, et al., Biochem.J. 2003, 376: 525-535.
2. N. Kachel, et al. J.Mol. Biol. 2003, 334: 143-155.