Aurora Kinase B

Aurora B
type : enzyme
pack size : 10 ug

Product Data: Aurora kinase B (AURKB) full length (human, recombinant), N-terminal GST-His6 Tag

Description: Human Aurora kinase B (GenBank Accession No. NM_004217), (a.a. 2- 344), expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Stability: 6 months at –80 °C

Purity: >80% by SDS-PAGE.

Specific Activity: 22 U/ug. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30 °C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 4 uM aurora B substrate, and 4 ug/ml Aurora B kinase.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

References:
1.Delaval, B. et al., Oncogene 23 (26), 4516-4522 (2004).
2.Yasui, Y. et al., J. Biol. Chem. 279, 12997-13003 (2004).