Akt1 (active)

Atk1 ACTIVE
type : enzyme
pack size : 10 ug

Product Data: Atk1 - active (human, recombinant, N-terminal His tag)

Description: Human Akt1 (GenBank Accession No. NM_001014432), (a.a. 1-480) with N-terminal HIS6 tag, MW=60.2 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Source: Baculovirus infected Sf9 cells

Formulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 3 mM DTT.

Stability: >6 months at –80°C

Purity: >80% by SDS-PAGE.

Specific Activity: 100 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of Crosstide substrate per minute at pH 7.4 and 30°C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

References:
1. Alessi et al., Current Biology 8:69, 1998
2. Cohen et al., FEBS Lett. 410:3, 1997