Small
interfering RNAs (siRNAs) are short, double-stranded RNA
molecules that can target and degradate specific
complementary mRNAs. The target gene-specific degradation is
an effective means of gene suppression. BioVision's
GeneBlocker
pGB siRNA expression vectors are designed to provide
efficient suppression of a target gene in cultured mammalian
cells and in vivo. The pGB vector has been optimized for
suppressing expression of target genes using the human U6
promotor (a RNA polymerase III promotor) which generates
large amounts of siRNA in mammalian cells. The pGB vector
provides neomycin resistance marker for the selection of
stable cell lines, permitting long term suppression of the
target gene. BioVision offers siRNA vectors targeting to
important Apoptosis genes. The negative control siRNA vector
and pGB cloning vector for cloning in your own insert are
also available.
Schematic Diagram of the RNA Interference Mechanism.
Once processed, the vector expressed siRNA is incorporated
into a multi-protein nuclease complex known as the
RNA-induced silencing complex (RISC). Homology between the
sense portion of the siRNA sequence and the mRNA enables the
nuclease enzyme to bind and cleave the caspase transcript
into small pieces, which are degraded by the cell's
machinery.
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pGB siRNA expression vectors are designed to provide
efficient suppression of a target gene in cultured mammalian
cells and in vivo. The pGB vector has been optimized for
suppressing expression of target genes using the human U6
promotor (a RNA polymerase III promotor) which generates
large amounts of siRNA in mammalian cells. The pGB vector
provides neomycin resistance marker for the selection of
stable cell lines, permitting long term suppression of the
target gene. BioVision offers siRNA vectors targeting to
important Apoptosis genes. The negative control siRNA vector
and pGB cloning vector for cloning in your own insert are
also available.