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Protein Kinase Assays



 
Activation of protein kinase, such as JNK and Akt, plays an important role in apoptosis and other physiological and pathological processes. BioVision provides a novel nonradioactive system for convenient measurement of JNK and Akt activities. The JNK Activity Screening Kit is used to screen JNK activity relatively quickly for large numbers of samples. The assay utilizes a N-terminal c-Jun fusion protein bead to selectively “pull down” JNK from cell lysate. c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. The KinaseSTARTM JNK or Akt Activity Assay Kit utilizes a JNK- (or Akt-) specific antibody to immunoprecipitate JNK (or Akt) from cell lysate. JNK- (or Akt-) specific activity is then analyzed by Western blot analysis of the phosphorylation levels of c-Jun (or GSK-3a) using a phospho-c-Jun (or phospho-GSK-3a) specific antibody. The kit specifically detects JNK (or Akt) activity, other kinase activities would not be detected.
 
 

 
Akt Activity Assay Kit
40 assays. Assay utilizes a Akt-specific antibody to immunoprecipitate Akt from cell lysate. Akt-specific activity is then analyzed by determining the phosphorylation of GSK-3alpha by Western blotting using a phospho-GSK-3alpha (Ser21) specific antibody. All reagents and antibodies necessary for the assays are included in the kit.
 
Product Name Akt Activity Assay K Stock# K435-40
KinaseSTAR JNK Activity Screening Kit
40 assays. Screen JNK activity relatively quickly for large numbers of samples. The assay utilizes a N-terminal c-Jun fusion protein bead to selectively pull down JNK from cell lysate. c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody. All reagents and antibodies necessary for the assay are included in the kit.
 
Product Name KinaseSTAR JNK Activ Stock# K430-40
KinaseSTAR JNK Activity Assay Kit
40 assays. The kit utilizes a JNK-specific antibody to immunoprecipitate JNK from cell lysate. JNK-specific activity is then analyzed by determining the phosphorylation of c-Jun by Western blotting using a phospho-c-Jun specific antibody. All reagents and antibodies necessary for the assays are included in the kit.
 
Product Name KinaseSTAR JNK Activ Stock# K431-40
c-Jun (1-79)-GST Fusion Protein
100 ug. Substrate for JNK.
 
Product Name c-Jun (1-79)-GST Fus Stock# 7001-100
GSK-3a-GST Fusion Protein
100 ug. Substrate for Akt.
 
Product Name GSK-3a-GST Fusion Pr Stock# 7003-100
Anisomycin
10 mg
 
Product Name Anisomycin Stock# 1549-10
Human TNF-alpha
50 ug
 
Product Name Human TNF-alpha Stock# 1050-50
Human TNF-alpha
10 ug
 
Product Name Human TNF-alpha Stock# 1050-10
Human TNF-alpha ELISA Kit
10 x 96 wells
Product Name Human TNF-alpha ELISA Kit Stock# K1050-1000
Murine TNF-alpha
10 ug
 
Product Name Murine TNF-alpha Stock# 1051-10
Murine TNF-alpha
20 ug
 
Product Name Murine TNF-alpha Stock# 1051-20
Murine TNF-alpha ELISA Kit
10 x 96 wells
Product Name Murine TNF-alpha ELISA Dev. Ki Stock# K1051-1000
Rat TNF-alpha
10 ug
 
Product Name Rat TNF-alpha Stock# 1052-10
Rat TNF-alpha
20 ug
 
Product Name Rat TNF-alpha Stock# 1052-20
Rat TNF-a ELISA Development Kit
10 x 96 well plates
 
Product Name Rat TNF-a ELISA Development Ki Stock# K1052-1000

L-JNK Peptide Inhibitor I
50 ul (1 mM) JNK peptide inhibitor. It is ready-to-use and cell permeable. It can be used in 1000X dilutions.
 
Product Name L-JNK Peptide Inhibi Stock# 7011-50
L-JNKI1 Negative Control Peptide
50 ul (1 mM) JNKI1 Negative Control Peptide. It is ready-to-use and cell permeable. Use in 1000X dilutions or use at the same concentration as JNKI1.
 
Product Name L-JNKI1 Negative Con Stock# 7021-50
Akt Inhibitor
1 mg
 
Product Name Akt Inhibitor Stock# 1701-1
JNK Negative Control Cell Lysate
1 mg Untreated Jurkat Cell Lysate. Use as a negative control for JNK activity assays.
 
Product Name JNK Negative Control Stock# 7031-1
JNK Activated Cell Lysate
1 mg anisomycin treated Jurkat cell lysate. Use as a positive control for JNK assay.
 
Product Name JNK Activated Cell L Stock# 7032-1
Akt Negative Control Lysate
1 mg Untreated Jurkat Cell Lysate. Use as a negative control for Akt activity assays.
 
Product Name Akt Negative Control Stock# 7035-1
Akt Activated Cell Lysate
1 mg. Treated Jurkat cell lysate. Use as positive control for Akt assay.
 
Product Name Akt Activated Cell L Stock# 7036-1
Active Akt1
5 ug highly active human Akt1. Purity >90%.
Product Name Active Akt1 Stock# 7701-5
Active Akt2
5 ug highly active human Akt2 expressed in Sf 9 cells.
Product Name Active Akt2 Stock# 7702-5
Active Akt3
5 ug highly active human Akt3 expressed in Sf9 cells. Purity > 90%.
Product Name Active Akt3 Stock# 7703-5
Active ASK1
5 ug highly active human ASK1 expressed in Sf 9 cells. Purity > 95%.
Product Name Active ASK1 Stock# 7710-5
Active BLK
5 ug highly active human BLK expressed in Sf 9 cells. Purity > 90%.
Product Name Active BLK Stock# 7723-5
Active BMX
5 ug highly active human BMX expressed in Sf 9 cells. Purity > 90%.
Product Name Active BMX Stock# 7732-5
Active BRK
5 ug highly active human BRK expressed in Sf 9 cells. Purity > 95%.
Product Name Active BRK Stock# 7708-5
Active CAMK1b
5 ug highly active human CAMK1b expressed in Sf 9 cells. Purity > 95%.
Product Name Active CAMK1b Stock# 7729-5
Active CAMK1d
5 ug highly active human CAMK1d expressed in Sf 9 cells. Purity > 90%.
Product Name Active CAMK1d Stock# 7713-5
Active CAMK1G
5 ug highly active human CAMK1G expressed in Sf 9 cells. Purity > 95%.
Product Name Active CAMK1G Stock# 7736-5
Active CAMK4
5 ug highly active human CAMK4 expressed in Sf 9 cells. Purity > 90%.
Product Name Active CAMK4 Stock# 7740-5
Active CHK1
5 ug highly active human CHK1 expressed in Sf 9 cells. Purity > 95%.
Product Name Active CHK1 Stock# 7735-5
Active CHK2
5 ug highly active human CHK2 expressed in Sf 9 cells. Purity > 90%.
Product Name Active CHK2 Stock# 7712-5
Active CK2a1
5 ug highly active human CK2a1 expressed in Sf 9 cells. Purity > 95%.
Product Name Active CK2a1 Stock# 7738-5
Active CSK
5 ug highly active human CSK expressed in Sf 9 cells. Purity > 90%.
Product Name Active CSK Stock# 7722-5
Active ERK1
5 ug highly active human ERK1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active ERK1 Stock# 7741-5
Active FGR
5 ug highly active human FGR expressed in Sf 9 cells. Purity > 90%.
Product Name Active FGR Stock# 7724-5
Active GRK5
5 ug highly active human GRK5 expressed in Sf 9 cells. Purity > 95%.
Product Name Active GRK5 Stock# 7709-5
Active Recombinant GSK-3b
100 ug. Effective phosphorylate GSK-3beta substrate.
 
Product Name Active Recombinant G Stock# 7004-100
Active HCK
5 ug highly active human HCK expressed in Sf 9 cells. Purity > 90%.
Product Name Active HCK Stock# 7720-5
Active Lck
5 ug highly active human Lck expressed in Sf 9 cells. Purity > 90%.
Product Name Active Lck Stock# 7733-5
Active Lyn B
5 ug highly active human Lyn B expressed in Sf 9 cells. Purity > 90%.
Product Name Active Lyn B Stock# 7715-5
Active MAPKAP kinase 2
5 ug highly active human MAPKAP kinase 2 expressed in Sf 9 cells. Purity > 90%.
Product Name Active MAPKAP kinase 2 Stock# 7737-5
Active NEK2
5 ug highly active human Nek2 expressed in Sf 9 cells. Purity > 90%.
Product Name Active NEK2 Stock# 7719-5
Active NEK6
5 ug highly active human Nek6 expressed in Sf 9 cells. Purity > 90%.
Product Name Active NEK6 Stock# 7716-5
Active NEK7
5 ug highly active human NEK7 expressed in Sf 9 cells. Purity > 90%.
Product Name Active NEK7 Stock# 7717-5
Active p70 S6K
5 ug highly active human p70 S6K expressed in Sf 9 cells. Purity > 90%.
Product Name Active p70 S6K Stock# 7725-5
Active PAK4
5 ug highly active human PAK4 expressed in Sf 9 cells. Purity > 95%.
Product Name Active PAK4 Stock# 7707-5
Active PAK7
5 ug highly active human PAK7 expressed in Sf 9 cells.
Product Name Active PAK7 Stock# 7727-5
Active PDK1
5 ug highly active human PDK1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active PDK1 Stock# 7706-5
Active Pim 1
5 ug highly active human Pim 1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active Pim 1 Stock# 7742-5
Active PIM2
5 ug highly active human PIM2 expressed in Sf 9 cells. Purity > 95%.
Product Name Active PIM2 Stock# 7730-5
Active PKA cg
5 ug highly active human PKA cg expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKA cg Stock# 7728-5
Active PKAca
5 ug highly active human PKAca expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKAca Stock# 7743-5
Active PKAcb
5 ug highly active human PKAcb expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKAcb Stock# 7744-5
Active PKC bII
5 ug highly active human PKC bII expressed in Sf9 cells. Purity > 90%.
Product Name Active PKC bII Stock# 7704-5
Active PKC delta
5 ug highly active human PKC delta expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKC delta Stock# 7739-5
Active PKC iota
5 ug highly active human PKC iota expressed in Sf9 cells. Purity > 90%.
Product Name Active PKC iota Stock# 7705-5
Active PKCa
5 ug highly active human PKCa expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKCa Stock# 7714-5
Active PKCeta
5 ug highly active human PKCeta expressed in Sf 9 cells. Purity > 95%.
Product Name Active PKCeta Stock# 7731-5
Active PKCmu
5 ug highly active human PKCmu expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKCmu Stock# 7745-5
Active PKCnu
5 ug highly active human PKCnu expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKCnu Stock# 7746-5
Active PKCzeta
5 ug highly active human PKCzeta expressed in Sf 9 cells. Purity > 90%.
Product Name Active PKCzeta Stock# 7718-5
Active PKD2
5 ug highly active human PDK2 expressed in Sf 9 cells.
Product Name Active PKD2 Stock# 7711-5
Active RAF1
5 ug highly active human RAF1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active RAF1 Stock# 7726-5
Active RIPK2
5 ug highly active human RIPK2 expressed in Sf 9 cells. Purity > 90%.
Product Name Active RIPK2 Stock# 7747-5
Active RSK1
5 ug highly active human RSK1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active RSK1 Stock# 7721-5
Active SGK1
5 ug highly active human SGK1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active SGK1 Stock# 7748-5
Active SGK2
5 ug highly active human SGK2 expressed in Sf 9 cells. Purity > 90%.
Product Name Active SGK2 Stock# 7749-5
Active Src1
5 ug highly active human Src1 expressed in Sf 9 cells. Purity > 90%.
Product Name Active Src1 Stock# 7750-5
Active STK3
5 ug highly active human STK3 expressed in Sf 9 cells. Purity > 90%.
Product Name Active STK3 Stock# 7751-5
Active ZAP70
5 ug highly active human ZAP70 expressed in Sf 9 cells. Purity > 90%.
Product Name Active ZAP70 Stock# 7752-5

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07