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Mitochondrial Assays

Mitochondrial Apoptosis

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ADP/ATP Ratio Assay Kit
200 assays. Designed to detect ADP/ATP ratios for a rapid screening of apoptosis/necrosis/growth arrest/cell proliferation simultaneously in mammalian cells. Offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). Assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).
 
Product Name ADP/ATP Ratio Assay Stock# K255-200
MitoCapture Apoptosis Detection Kit
25 assays. Provides a simple assay for detecting apoptosis in living cells. In healthy cells MitoCapture accumulates in the mitochondria-fluorescing bright red. In apoptotic cells MitoCapture remains in the cytoplasm in its monomer form-fluorescing green. The fluorescent signals can be detected by fluorescence microscopy or flow cytometry.
 
Product Name MitoCapture Apoptosi Stock# K250-25
MitoCapture Apoptosis Detection Kit
100 assays. Highly sensive assay for detecting apoptosis in living cells. See detailed descriptions above in K250-25.
 
Product Name MitoCapture Apoptosi Stock# K250-100
MitoCapture Incubation Buffer
200 ml. Buffer used for mitocapture assays.
Product Name MitoCapture Incubati Stock# 1065-200

Cytochrome c Assays

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Cytochrome c
100 mg. >95% pure and no TCA.
Product Name Cytochrome c Stock# 2120-100
Cytochrome c Apoptosis Assay Kit
100 assays. Provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit separates a mitochondria-enriched fraction from cytosol and then cytochrome c translocation is then determined by Western blotting using the cytochrome c antibody provided in the kit.
 
Product Name Cytochrome c Apoptos Stock# K257-100
Cytochrome c Monoclonal Ab
100 ug purified mouse monoclonal antibody. Detects 12 kDa cytochrome c in denatured human/mouse/rat samples. Tested in Western blot analysis.
 
Product Name Cytochrome c Monoclo Stock# 3026-100
Cytochrome c Polyclonal Antibody
100 ug Rabbit Polyclonal Antibody. Reacts with rat. Tested in WB.
 
Product Name Cytochrome c Polyclo Stock# 3352-100
Cytochrome c Polyclonal Antibody
100 ug Rabbit Polyclonal Antibody. Reacts with mouse. Tested in WB.
 
Product Name Cytochrome c Polyclo Stock# 3353-100
Cytochrome p450 Polyclonal Ab
100 ug rabbit polyclonal antibody. Detects cytochrome P450 from human/mouse/rat/dog/horse samples. Perform well in Western blotting and immunohistochemistry.
 
Product Name Cytochrome p450 Poly Stock# 3084-100

Cell Proliferation

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ADP/ATP Ratio Assay Kit
200 assays. Designed to detect ADP/ATP ratios for a rapid screening of apoptosis/necrosis/growth arrest/cell proliferation simultaneously in mammalian cells. Offers highly consistent results and with excellent correlation to other apoptosis markers (e.g. TUNEL-based assays and caspase assays). Assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 cells/well).
 
Product Name ADP/ATP Ratio Assay Stock# K255-200
ATP Cell Viability Assay Kit
200 assays. Utilizes bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells. The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations and can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
 
Product Name ATP Cell Viability A Stock# K254-200
ATP Cell Viability Assay Kit
1000 assays. See the description above in K254-200.
 
Product Name ATP Cell Viability A Stock# K254-1000
Bioluminescence Cytotoxicity Assay Kit
500 assays. Sensitive detection of cytotoxicity by luminescence method.
 
Product Name Bioluminescence Cyto Stock# K312-500
Live-Dead Cell Staining Kit
100 stainings. Provides ready-to-use staining reagents and incubation buffer for distinguishing between live and dead cells in culture. Contains a cell-permeable green fluorescent dye (Ex/Em = 490/515 nm) for staining live cells and propidium iodide (PI Ex/Em = 488/615 nm) for staining dead cells. Stained lice and dead cells can easily be visualized by fluorescence microscopy.
 
Product Name Live-Dead Cell Stain Stock# K501-100
Quick Cell Proliferation Assay Kit
500 assays. This fast and sensitive assay measures cell proliferation in response to growth factors/cytokines/mitogens/and nutrients etc. The method is so simple requiring no washing/harvesting/or solubilization steps and is faster and more sensitive than MTT/XTT/or MTS-based assays. The entire assay can be performed directly in a 96-well plate.
 
Product Name Quick Cell Prolifera Stock# K301-500
Quick Cell Proliferation Assay Kit
2500 assays. See the descriptions above in K301-500.
 
Product Name Quick Cell Prolifera Stock# K301-2500

ApoSENSOR ATP Cell Viability Assay Kit

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ATP Cell Viability Assay Kit
200 assays. Utilizes bioluminescent detection of the ATP level via luciferase catalyzed reaction for a rapid screening of apoptosis and cell viability in mammalian cells. The assay can be done directly in culture plates requiring no harvest/washing/or sample preparations and can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
 
Product Name ATP Cell Viability A Stock# K254-200
ATP Cell Viability Assay Kit
1000 assays. See the description above in K254-200.
 
Product Name ATP Cell Viability A Stock# K254-1000

Glutathione Assays

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Glutathione Colorimetric Assay Kit
100 assays. Provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes by colorimetric method.
 
Product Name Glutathione Colorime Stock# K261-100
Glutathione Fluorometric Assay Kit
100 assays. Provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes by fluorometric method (excitation = 380 nm and emission = 461 nm) using a fluorometer or a 96-well fluorometric plate reader.
 
Product Name Glutathione Fluorome Stock# K251-100
Oxidized Glutathione (GSSG)
1 g
 
Product Name Oxidized Glutathione Stock# 1241-1
Reduced Glutathione (GSSG)
1 g
 
Product Name Reduced Glutathione Stock# 1242-1

GST Assays

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Active GST Protein
1 mg
 
Product Name Active GST Protein Stock# 1243-1
GST Colorimetric Assay Kit
100 assays. Detecting GST activity by spectrophotometer at 340 nm.
 
Product Name GST Colorimetric Ass Stock# K263-100
GST Fluorometric Assay Kit
100 assays. Detecting GST activity by fluorometer or fluorescence plate reader at Ex/Em 380/461 nm.
 
Product Name GST Fluorometric Ass Stock# K260-100
GST Inhibitor-1 (Cibacron Blue F3G-A)
1 g
 
Product Name GST Inhibitor-1 (Cib Stock# 1555-1000
GST Inhibitor-2 (Ethacrynic acid)
1 g
 
Product Name GST Inhibitor-2 (Eth Stock# 1556-1000

Nitric Oxide Assays

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Nitric Oxide Colorimetric Assay Kit
2 x 96 assays. Provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions in a 96-well plate. Suitable for measurements of total NO in culture media/urine/plasma/serum as well as many other biological fluids.
 
Product Name Nitric Oxide Colorim Stock# K262-200
Nitric Oxide Fluorometric Assay Kit
2 x 96 assays. Provides a simple two-step process for quantitative measurement of total nitrate/nitrite productions in 96-well plate. Suitable for measurements of total NO in culture media/urine/plasma/serum as well as many other biological fluids.
 
Product Name Nitric Oxide Fluorom Stock# K252-200

Cell Fractionation

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Cytosol/Particulate Separation Kit
50 assays. Provides an easy system for rapid separation of cytosol and particulate fractions that are compatiable with downstream studies.
 
Product Name Cytosol/Particulate Stock# K267-50
FractionPREP Cell Fractionation Kit
50 assays. Designed for serial preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions from one sample. All these fractions can directly be used in downstream assays such as 2-D gel/Western blot/gel-shift/translocation/enzyme assay/reporter assay.
 
Product Name Cell Fractionation Stock# K270-50
Mammalian Cell Extraction Kit
500 assays. Provides optimized buffer and reagents for convenient extraction of mammalian proteins from tissues and cells under nondenaturing conditions. Cell Lysates can be used in a variety of enzyme activity assays. Example includes caspase assays and Western blot analyses.
 
Product Name Mammalian Cell Extra Stock# K269-500
Membrane Protein Extraction Kit
50 assays. Provides an easy and complete protocol for isolating integral membrance proteins efficiently from cultured mammalian cells. The isolated membrance fractions can directly be used in SDS-PAGE and Western blotting or for further purifying specific membrance proteins.
 
Product Name Membrane Protein Ext Stock# K268-50
Mitochondria/Cytosol Fractionation Kit
100 assays. Provides unique formulations of buffers and reagents for separating a highly enriched mitochondrial fraction from cytosol of mammalian cells. No ultracentrifugations or toxic chemicals involved. enriched fractions are compatible for down-stream assays.
 
Product Name Mitochondria/Cytosol Stock# K256-100
Mitochondrial DNA Isolation Kit
50 assays. Provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction. Mitochondrial DNA are then isolated using reagents included in the kit.
 
Product Name Mitochondrial DNA Is Stock# K280-50
Nuclear/Cytosol Fractionation Kit
100 assays. Provides a complete system for separating nuclear fraction from the cytoplasm of mammalian cells and tissues. The system maintains the nuclear and cytoplasmic compartments separate and intact with little or no cross-contaminations. Extracted fractions are compatible with downstream assays.
 
Product Name Nuclear/Cytosol Frac Stock# K266-100
Dounce Tissue Grinder
1 ea. Use in conjunction with Cell Fractionation Kits (K256, K257, K266, K268, K269, K270).
 
Product Name Dounce Tissue Grinder Stock# 1998-1

DNA Isolation

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Mitochondrial DNA Isolation Kit
50 assays. Provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction. Mitochondrial DNA are then isolated using reagents included in the kit.
 
Product Name Mitochondrial DNA Is Stock# K280-50
Genomic DNA Isolation Kit
50 assays. Provides unique formulations of buffers and reagents for convenient isolation of highly pure genomic DNA.
 
Product Name Genomic DNA Isolatio Stock# K281-50
 
 

 

For more information :: Bioxys and Gentaur BVBA :: Av. de l'Armée 68 B4 :: BE-1040 BELGIUM

Email: info@gentaur.com
 

International 

+32 (0) 16 58 90 45

+32 (0) 16 50 90 45

France

01 43 25 01 50

01 43 25 01 60

Italy

02 36 00 65 93

02 36 00 65 94

Germany

0241 6085 13140

0241 6085 33033
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Last modified: feb-07