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Inhibition of
histone deacetylases (HDACs) has been implicated to modulate
transcription and to induce apoptosis or differentiation in
cancer cells. The HDAC Fluorometric and Colorimetric
Assay Kits provide simple procedures for fast and convenient
measurement of HDAC activity by fluorometric and
colorimetric methods. The methods eliminate radioactivity,
extractions, or chromatography, as used in the traditional
assays. The new procedures require only two easy steps, both
performed on the same microtiter plate. First, the HDAC
fluorometric (or colorimetric) substrate, which comprises an
acetylated lysine side chain, is incubated with a sample
containing HDAC activity (e.g., HeLa nuclear extract).
Deacetylation sensitizes the substrate, and thus in the
second step, treatment with the Lysine Developer produces a
fluorophore (or chromophore), which can be analyzed using a
fluorometric (or colorimetric) plate reader. The HDAC
Inhibitor Drug Screening Kit provides sufficient positive
controls for screening 100 samples of HDAC inhibitor
candidates in drug discovery. HDAC inhibitors and antibodies
are also available separately.
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