Glutathione Assay Kits

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The ApoGSH(TM) Glutathione Colorimetric Assay Kit provides a convenient, colorimetric method for analyzing either total glutathione or only the reduced form of glutathione using a microtiter plate reader. The assay utilizes the glutathione recycling system by DTNB and glutathione reductase (as shown below), which dramatically improves the sensitivity of total glutathione detection. The kit can quantify total glutathione from 1-100 ng/well. For detecting lower glutathione concentrations, such as in blood samples, increasing reaction time will significant improve the signal. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture.

ApoGSH^TM Colorimetric Glutathione Detection Kit

Kit Components:

Glutathione Reaction Buffer

Glutathione Substrate

NADPH Generating Mix

Glutathione Reductase

Sulfosalicylic Acid

GSH Standard

User Manual

Glutathione Standard Calibration Curve.
10 minutes incubation at room temperature.

The ApoGSH^TM Glutathione Fluorometric Assay Kit provides a simple, fluorescence-based in vitro assay for detecting the GSH changes in apoptosis and other cellular processes. The assay utilizes monochlorobimane (MCB), a dye that has a high affinity for glutathione. The unbound MCB is almost nonfluorescent, whereas the dye fluoresces blue (ex./em. = 380/461 nm) when bound to glutathione. Thus, the change in glutathione level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. The kit contains all the necessary components for the convenient analyses of glutathione within 30 minutes.

ApoGSH^TM Fluorometric Glutathione Detection Kit

Kit Components:

Cell Lysis Buffer

Monochlorobimane

GST Reagent

User Manual

GSH Level Decreases Following Induction of Apoptosis. Jurkat cells were treated with camptothecin for 8 hours. GSH level was measured using the ApoGSHTM Fluorometric kit according to kit instructions.

Glutathione Colorimetric Assay Kit

100 assays. Provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes by colorimetric method.

Product Name

Glutathione Colorime

Stock#

K261-100

Glutathione Fluorometric Assay Kit

100 assays. Provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes by fluorometric method (excitation = 380 nm and emission = 461 nm) using a fluorometer or a 96-well fluorometric plate reader.

Product Name

Glutathione Fluorome

Stock#

K251-100

Glutathione Assay Kits

Send mail to webmaster with questions or comments about this web site. Copyright © 2005 Gentaur BVBA Last modified: feb-07

Send mail to webmaster with questions or comments about this web site.
Copyright © 2005 Gentaur BVBA
Last modified: feb-07