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DNA Ladder/TUNEL-based Kits
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BioVision offers several novel kits for fast and
sensitive detection of DNA ladder in apoptotic cells.
Unlike other similar kits in the market which require 1-2
days to prepare nucleic acids, the new procedure requires
less than 90 minutes to prepare chromosomal DNA,
requiring neither extraction nor a column. The newly
released Apoptotic DNA ladder extraction kit can detect
apoptotic DNA ladder from tissue and cells that contain even
5% apoptotic cell population. DNA ladders can easily be
visualized by agarose gel electrophoresis.
The TUNEL-based DNA fragmentation assay kits provide
complete components including positive and negative control
cells or tissue slides for convenient detection of DNA
fragmentation in cultured cells and tissue sections. Results
can be analyzed by flow cytometry or fluorescence
microscopy.
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Quick Detection of Apoptotic DNA Ladder in
Jurkat Cells. Apoptosis was induced in
Jurkat cells with camptothecin (2 mM) for 0 hr
(Lane 1), 6 hrs (Lane 2) and 12 hrs (Lane 3).
Chromosomal DNA was prepared using the Quick
Apoptotic DNA Ladder Detection Kit according to
the kit instructions. 20
ml
of each sample was electrophoresed on a 1.2%
agarose/EtBr gel.
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Detection of Apoptotic Cells Using Apo-BrdUTM
Kit by Flow Cytometer. Apoptosis was induced
in Jurkat cells with camptothecin (2 mM) for 8
hours. Control cells (uninduced) and induced
cells were both collected, labeled with ApoBrdU
according to the kit instructions. Results were
analyzed by flow cytometry.
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Nuclear Apoptosis
Cell Fractionation
DNA Isolation
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45