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The Cholesterol/Cholesteryl Ester Quantitation Kit
provides a simple method for sensitive quantification of
cholesterol, cholesteryl Ester, or both by either
colorimetric or fluorometric methods. A large portion of
the cholesterol in blood is in the form of cholesteryl
esters. Cholesterol esterase hydrolizes cholesteryl ester
into cholesterol. Cholesterol is then oxidized by
cholesterol oxidase to yield H2O2.
The produced H2O2
interacts with a sensitive cholesterol probe to produce
resorufin, which can be detected by spectrophotometry at
l
= 570 nm or fluorometry at Ex/Em = 535/587 nm. The assay can
detect cholesterol itself (without adding cholesterol
esterase) or total cholesterol (cholesterol + cholesteryl
ester) by adding cholesterol esterase to the reaction, or
cholesteryl Ester itself by subtracting the value of
cholesterol from the total value of cholesterol and
cholesteryl esters.
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+32 (0) 16
58 90 45 
+32 (0) 16
50 90 45
Cholesterol Quantitation Kit
