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Adenosine 3’,5’-cyclic monophosphate (cyclic AMP, cAMP)
is one of the most important “second messengers” involved as
a modulator of physiological processes, such as regulating
neuronal, glandular, cardiovascular, immune mechanism,
nervous system, cell growth and differentiation. A
number of hormones (e.g., ACTH, TSH, LH) are known to
activate cAMP through the action of the enzyme adenylate
cyclase which converts ATP to cAMP. Guanosine 3,5-cyclic
monophosphate (cyclic GMP, cGMP) has been shown to be
present at levels typically 10-100 fold lower than cAMP in
most tissues and is formed by the action of the enzyme
guanylate cyclase on GTP. Some hormones (e.g.,
acetylcholine, insulin, and oxytocin) and chemicals (e.g.,
serotonin and histamine) cause an increase in cGMP levels.
BioVision’s cAMP and cGMP Assay Kits are the competitive
immunoassays for the quantitative determination of cAMP and
cGMP levels, respectively. The kits utilize polyclonal
antibody to cAMP (or cGMP) to bind, in a competitive manner,
the cAMP (or GMP) in the standards or samples. After a
simultaneous incubation the excess reagents are washed away
and substrate is added. After a short incubation time, the
enzyme reaction is stopped and the blue color generated can
be read on a microplate reader at 650 nm. The intensity of
the color is inversely proportionally to the concentration
of cAMP (or cGMP) in either standard or samples.
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cAMP Assay Kit
