Number :   
    30025-2
Product Name :   
     AlamarBlueTM Cell Viability Assay Kit

 

Kit Components:

  • AlamarBlueTM solution

    Description:

    AlamarBlueTM Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. AlamarBlueTM detects cell viability by utilizing a nonfluorescent dye resazurin, which is converted to a fluorescent dye resorufin (See Figure B9) in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the REDOX indicator to change from oxidized (nonfluorescent, blue) form to reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample.

    Features:

  • Sensitive: Requiring as few as 80 cells for reproducible results.
  • Simple: Single-step homogenous assay.
  • Kinetic Monitoring: Reagents nontoxic to cells, thus allowing kinetic monitoring.

    Related Products:

    Reference:

    1) J. Immunol. Methods 213, 157(1998); 2) J. Clin. Lab. Anal. 9, 89(1995); 3) J. Immunol. Methods 175, 181(1994).

    AlamarBlue is a trademark of Serotec, Inc.

    		•
    Catelog Number :   
    		    30025-1
    Product Name :   
    		     AlamarBlueTM Cell Viability Assay Kit

     

    Kit Components:

  • AlamarBlueTM solution

    Description:

    AlamarBlueTM Cell Viability Assay offers a simple, rapid, reliable, sensitive, safe and cost-effective measurement of cell viability. AlamarBlueTM detects cell viability by utilizing a nonfluorescent dye resazurin, which is converted to a fluorescent dye resorufin (See Figure B9) in response to chemical reduction of growth medium resulting from cell growth. Continued cell growth maintains a reduced environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the REDOX indicator to change from oxidized (nonfluorescent, blue) form to reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample.

    Features:

  • Sensitive: Requiring as few as 80 cells for reproducible results.
  • Simple: Single-step homogenous assay.
  • Kinetic Monitoring: Reagents nontoxic to cells, thus allowing kinetic monitoring.

    Related Products:

    Reference:

    1) J. Immunol. Methods 213, 157(1998); 2) J. Clin. Lab. Anal. 9, 89(1995); 3) J. Immunol. Methods 175, 181(1994).

    AlamarBlue is a trademark of Serotec, Inc.

    		•
    Catelog Number :   
    		    30020-4
    Product Name :   
    		     ATP-GloTM Bioluminometric Cell Viability Assay Kit

     

    Kit Components:

    • ATP-GloTM substrate
    • ATP-GloTM buffer

    Description:

    ATP-GloTM Cell Viability Assay offers a homogenous assay for quantifying the number of viable cells based on the presence of ATP, an indicator of metabolically active cells. ATP-GloTM is based on Firefly luciferase? requirement for ATP in producing light from the oxidation reaction of D-luciferin (See Figure B11). The assay is highly sensitive, detecting as little as 1 picomole of ATP. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from determinations of viable cell numbers, to cell proliferation and to cytotoxicity. This assay can be performed using a standard luminometer and is also compatible with high-throughput screening (HTS).

    Features:

    • High Sensitivity and Extended Linearity: Detect from 50 or fewer cells to several million cells with excellent linearity.
    • Simple: A single-step homogenous assay.
    • Robust & Amenable to HTS: Stable luminescent signal with excellent Z'-factor values for screening applications.

    Related Products:

    See Section 6 of the catalog for a complete list of D-luciferin products.

    Reference:

    1) J. Biolumin. Chemilumin. 10, 29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol. Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991); 5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160, 81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986).
    Catelog Number :   
    		    30020-3
    Product Name :   
    		     ATP-GloTM Bioluminometric Cell Viability Assay Kit

     

    Kit Components:

    • ATP-GloTM substrate
    • ATP-GloTM buffer

    Description:

    ATP-GloTM Cell Viability Assay offers a homogenous assay for quantifying the number of viable cells based on the presence of ATP, an indicator of metabolically active cells. ATP-GloTM is based on Firefly luciferase? requirement for ATP in producing light from the oxidation reaction of D-luciferin (See Figure B11). The assay is highly sensitive, detecting as little as 1 picomole of ATP. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from determinations of viable cell numbers, to cell proliferation and to cytotoxicity. This assay can be performed using a standard luminometer and is also compatible with high-throughput screening (HTS).

    Features:

    • High Sensitivity and Extended Linearity: Detect from 50 or fewer cells to several million cells with excellent linearity.
    • Simple: A single-step homogenous assay.
    • Robust & Amenable to HTS: Stable luminescent signal with excellent Z'-factor values for screening applications.

    Related Products:

    See Section 6 of the catalog for a complete list of D-luciferin products.

    Reference:

    1) J. Biolumin. Chemilumin. 10, 29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol. Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991); 5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160, 81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986).
    Catelog Number :   
    		    30020-2
    Product Name :   
    		     ATP-GloTM Bioluminometric Cell Viability Assay Kit

     

    Kit Components:

    • ATP-GloTM substrate
    • ATP-GloTM buffer

    Description:

    ATP-GloTM Cell Viability Assay offers a homogenous assay for quantifying the number of viable cells based on the presence of ATP, an indicator of metabolically active cells. ATP-GloTM is based on Firefly luciferase? requirement for ATP in producing light from the oxidation reaction of D-luciferin (See Figure B11). The assay is highly sensitive, detecting as little as 1 picomole of ATP. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from determinations of viable cell numbers, to cell proliferation and to cytotoxicity. This assay can be performed using a standard luminometer and is also compatible with high-throughput screening (HTS).

    Features:

    • High Sensitivity and Extended Linearity: Detect from 50 or fewer cells to several million cells with excellent linearity.
    • Simple: A single-step homogenous assay.
    • Robust & Amenable to HTS: Stable luminescent signal with excellent Z'-factor values for screening applications.

    Related Products:

    See Section 6 of the catalog for a complete list of D-luciferin products.

    Reference:

    1) J. Biolumin. Chemilumin. 10, 29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol. Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991); 5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160, 81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986).
    Catelog Number :   
    		    30020-1
    Product Name :   
    		     ATP-GloTM Bioluminometric Cell Viability Assay Kit

     

    Kit Components:

    • ATP-GloTM substrate
    • ATP-GloTM buffer

    Description:

    ATP-GloTM Cell Viability Assay offers a homogenous assay for quantifying the number of viable cells based on the presence of ATP, an indicator of metabolically active cells. ATP-GloTM is based on Firefly luciferase? requirement for ATP in producing light from the oxidation reaction of D-luciferin (See Figure B11). The assay is highly sensitive, detecting as little as 1 picomole of ATP. By relating the amount of ATP to the number of viable cells, the assay has wide applications, ranging from determinations of viable cell numbers, to cell proliferation and to cytotoxicity. This assay can be performed using a standard luminometer and is also compatible with high-throughput screening (HTS).

    Features:

    • High Sensitivity and Extended Linearity: Detect from 50 or fewer cells to several million cells with excellent linearity.
    • Simple: A single-step homogenous assay.
    • Robust & Amenable to HTS: Stable luminescent signal with excellent Z'-factor values for screening applications.

    Related Products:

    See Section 6 of the catalog for a complete list of D-luciferin products.

    Reference:

    1) J. Biolumin. Chemilumin. 10, 29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol. Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991); 5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160, 81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986).
    Catelog Number :   
    		    30026
    Product Name :   
    		     Calcein AM Cell Viability Assay Kit

     

    Kit Components:

  • Calcein AM in anhydrous DMSO

    Description:

    Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM, a nonfluorescent molecule itself, is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein. The fluorescent calcein is retained in the cytoplasm in live cells. Calcein AM has been served as an excellent tool for the studies of cell membrane integrity and for long-term cell tracing. Calcein AM Cell Viability Assay Kit is designed to quantify live cell numbers based on the presence of their cytoplasmic membrane integrity. It is a true end-point assay for cell viability. The fluorescent signal is monitored using 485 nm excitation wavelength and 530 nm emission wavelength. The fluorescent signal generated from the assay is proportional to the number of living cells in the sample (See Figure B10).

    Features:

    • True end-point viability assay: Detect only cells with uncompromised cytoplasmic membrane integrity.
    • Simple & Fast: A single 30-min assay.

    Spectra:

    See #80013 for spectra.

    Related Products:

    • Calcein, 100 mg (#80013)
    • Calcein AM, 1mg (#80011)
    • Calcein AM, 100 uL at 4 mM in DMSO (#80011-1)
    • Calcein AM, 1mL at 1mg/mL in DMSO (#80011-2)
    • Calcein AM, 20x50 ug (#80011-3)

    Reference:

    1) J. Neurosci. Methods. 50, 205(1993); 2) J. Immunol. Methods 163,181(1993); 3) Cancer Lett. 87, 199(1994); 4) J. Immunol. Methods 178, 41(1995)
    		•
    Catelog Number :   
    		    30006
    Product Name :   
    		     MTT Cell Viability Assay Kit

     

    Kit Components:

  • MTT solution

    Description:

    MTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using a standard colorimetric plate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and cell cytotoxicity caused by drugs and cytotoxic agents. Among all non-radioactive viability assays, MTT assay developed by Mossman is one of the most versatile and popular assays. MTT is a tetrazolium salt that is turned into a purple formazan product after reduction by mitochondrial enzymes that are only present in metabolically active live cells, not in dead cells. The amount of formazan product generated is proportional to the number of living cells in the sample. The formazan product can be solubilized and then photometrically quantified at 570 nm.

    Features:

  • Simple: Ready-to-use MTT solution.
  • Flexible: Compatible with a standard spectrophotometer or a photometric plate reader for high throughput assays.

    Related Products:

    Reference:

    1) J Immunol. Methods 174, 311(1994); 2) J Immunol. Methods 145, 199(1991); 3) J Immunol. Methods 131, 165(1990); 4) Cancer Res. 48, 598(1988).
    		•
    Catelog Number :   
    		    30027
    Product Name :   
    		     Viability/Cytotoxicity Assay kit for Bacteria Live and Dead Cells

     

    Kit Components:

    • 2 vials (100 uL/vial): DMAO, 5 mM
    • 2 vials (150 uL/vial): Ethidium Homodimer-III (EthD-III), 2 mM

    Description:

    This kit provides a two-color fluorescence staining on both live bacteria (green) and dead bacteria (red) using two probes DMAO and EtD-III. DMAO is a green-fluorescent nucleic acid dye which stains both live and dead bacteria with intact and damaged cell membranes. EtD-III is a red-fluorescent nucleic acid dye that only stains dead bacteria with damaged cell membranes. With an appropriate mixture of DMAO and EtD-III, bacteria with intact cell membranes stain fluorescent green, whereas bacteria with damaged cell membranes stain fluorescent red. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types.

    Features:

    • Dual Detection: Detect live and dead bacteria cells in a cell population simultaneously.
    • Simple & Fast: 15 min dye loading and measure without washing.
    • Economic: Perform viability and cytotoxicity assays at the same time.
    • Versatile: Analysis compatiable with flow cytometers and fluorescence microscopes using popular settings for fluorescein and propidium iodide.

    Related Products:

    Viability/Cytotoxicity Staining Kit for Mammalian Live & Dead Cells (Cat.# 30002)

    Reference:
    Catelog Number :   
    		    30002
    Product Name :   
    		     Viability/Cytotoxicity Staining Kit for Animal Live & Dead Cells

     

    Kit Components:

    • 2 x 50 uL Calcein AM (4 mM)
    • 2 x 150 uL EthD-III (2 mM)

    Description:

    Viability/cytotoxicity Staining Kit for Animal Live & Dead Cells provides a two-color fluorescent staining of live (green) and dead cells (red) using two probes. Calcein AM stains live cells green while EthD-III stains dead cells red. These probes measure two recognized parameters of cell viability ? intracellular esterase activity and plasma membrane integrity (1,2). The kit is suitable for use with fluorescence microscopes, fluorescence multi-well plate scanners and flow cytometers. The assay principles are general and applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not to bacteria or yeast. This fluorescence-based method of assessing cell viability can be used in place of trypan blue exclusion, 51Cr release and similar methods for determining cell viability and cytotoxicity. EthD-III (# 40050 and #40051) is a proprietary DNA stain developed by Biotium and is a superior alternative to EthD-I offered by our competitors. EthD-III has higher DNA binding affinity and thus brighter fluorescence (70% brighter than Ethidium homodimer I).

    Features:

    • Dual Detection: Detect both live and dead cells simultaneously.
    • Simple & Fast: Require only a 30-min dye loading time and then measure without washing.
    • Economical: Perform viability and cytotoxicity assays at the same time.
    • Versatile: Analyze with flow cytometers, fluorescence microscopes or fluorescence plate readers.

    Related Products:

    1) Calcein AM, 1 mg (Cat. # 80011); 2) Calcein AM, 4mM, 100 uL (Cat. # 80011-1); 3) Calcein AM, 1 mg/mL (Cat. # 80011-2); 4) Calcein AM, 20 x 50 ug (Cat. # 80011-3); 5) Ethidium Homodimer I (EthD-I or EtDi), 1mg (Cat. # 40010); 6) EthD-I, 2 mM, 0.5 mL (Cat. # 40014 ); 7) EthD-III, 1mg (Cat. # 40050); 8) EthD-III, 1mM, 0.2 mL (Cat. # 40051)

    Reference:

    Biotechniques 29, 874-880 (2000)
    Catelog Number :   
    		    30007
    Product Name :   
    		     XTT Cell Viability Assay Kit

     

    Kit Components:

  • XTT solution
  • Activation Reagent

    Description:

    XTT Cell Viability Assay Kit provides a simple method for determining live cell numbers using standard photometric microplate readers. Determination of live cell numbers is often used to assess the rate of cell proliferation and to screen cytotoxic agents. XTT is a tetrazolium derivative. Similar to MTT, XTT measures cell viability based on the activity of mitochondrial enzymes in live cells that reduce XTT and are inactivated shortly after cell death. Unlike MTT, XTT is reduced to a highly water-soluble orange-colored product instead of the insoluble formazan from MTT, thus eliminating the solubilization step required for the MTT assay. The amount of water-soluble product generated from XTT reduction is proportional to the number of living cells in the sample and can be photometrically quantified at 475 nm. Please see Table B1 for comparison with other Viability/ Cytotoxicity Assay Kits. environment while inhibition of growth maintains an oxidized environment. Reduction related to growth causes the REDOX indicator to change from oxidized (nonfluorescent, blue) form to reduced (fluorescent, red) form. The fluorescent signal is monitored using 530-560 nm excitation wavelength and 590 nm emission wavelength. The absorbance is monitored at 570 nm and 600 nm. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample.

    Features:

  • Simple: Homogenous assay using ready-to-use XTT and activation reagent solutions
  • Kinetic Monitoring: Continuous color development permitting measurements at multiple time points for extended detection range.

    Related Products:

    Reference:

    1) J. Infect. dis. 172, 1153(1995); 2) J Immunol. Methods 159, 81(1993); 3) J Immunol. Methods 147, 153(1992); 4) J Immunol. Methods 142, 257(1991); 5) J. Natl. Cancer Inst. 81, 577(1989); 6) Cancer Res. 48, 4827(1988).
    		•