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Viability / Cytotoxicity Assays
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Number :
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30025-2 |
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Product Name :
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AlamarBlueTM
Cell Viability Assay Kit |
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Kit Components:
AlamarBlueTM solution
Description:
AlamarBlueTM Cell Viability
Assay offers a simple, rapid, reliable, sensitive, safe and
cost-effective measurement of cell viability. AlamarBlueTM
detects cell viability by utilizing a nonfluorescent dye
resazurin, which is converted to a fluorescent dye resorufin
(See Figure B9) in response to chemical reduction of growth
medium resulting from cell growth. Continued cell growth
maintains a reduced environment while inhibition of growth
maintains an oxidized environment. Reduction related to
growth causes the REDOX indicator to change from oxidized
(nonfluorescent, blue) form to reduced (fluorescent, red)
form. The fluorescent signal is monitored using 530-560 nm
excitation wavelength and 590 nm emission wavelength. The
absorbance is monitored at 570 nm and 600 nm. The
fluorescent or colorimetric signal generated from the assay
is proportional to the number of living cells in the sample.
Features:
Sensitive: Requiring as few as 80 cells for
reproducible results.
Simple: Single-step homogenous assay.
Kinetic Monitoring: Reagents nontoxic to cells, thus
allowing kinetic monitoring.
Related Products:
Reference:
1) J. Immunol. Methods 213,
157(1998); 2) J. Clin. Lab. Anal. 9, 89(1995); 3) J.
Immunol. Methods 175, 181(1994).
AlamarBlue is a trademark of Serotec, Inc.
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Catelog Number :
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30025-1 |
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Product Name :
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AlamarBlueTM
Cell Viability Assay Kit |
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Kit Components:
AlamarBlueTM solution
Description:
AlamarBlueTM Cell Viability
Assay offers a simple, rapid, reliable, sensitive, safe and
cost-effective measurement of cell viability. AlamarBlueTM
detects cell viability by utilizing a nonfluorescent dye
resazurin, which is converted to a fluorescent dye resorufin
(See Figure B9) in response to chemical reduction of growth
medium resulting from cell growth. Continued cell growth
maintains a reduced environment while inhibition of growth
maintains an oxidized environment. Reduction related to
growth causes the REDOX indicator to change from oxidized
(nonfluorescent, blue) form to reduced (fluorescent, red)
form. The fluorescent signal is monitored using 530-560 nm
excitation wavelength and 590 nm emission wavelength. The
absorbance is monitored at 570 nm and 600 nm. The
fluorescent or colorimetric signal generated from the assay
is proportional to the number of living cells in the sample.
Features:
Sensitive: Requiring as few as 80 cells for
reproducible results.
Simple: Single-step homogenous assay.
Kinetic Monitoring: Reagents nontoxic to cells, thus
allowing kinetic monitoring.
Related Products:
Reference:
1) J. Immunol. Methods 213,
157(1998); 2) J. Clin. Lab. Anal. 9, 89(1995); 3) J.
Immunol. Methods 175, 181(1994).
AlamarBlue is a trademark of Serotec, Inc.
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Catelog Number :
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30020-4 |
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Product Name :
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ATP-GloTM
Bioluminometric Cell Viability Assay Kit |
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Kit Components:
- ATP-GloTM substrate
- ATP-GloTM buffer
Description:
ATP-GloTM Cell Viability
Assay offers a homogenous assay for quantifying the number
of viable cells based on the presence of ATP, an indicator
of metabolically active cells. ATP-GloTM is based
on Firefly luciferase? requirement for ATP in producing
light from the oxidation reaction of D-luciferin (See Figure
B11). The assay is highly sensitive, detecting as little as
1 picomole of ATP. By relating the amount of ATP to the
number of viable cells, the assay has wide applications,
ranging from determinations of viable cell numbers, to cell
proliferation and to cytotoxicity. This assay can be
performed using a standard luminometer and is also
compatible with high-throughput screening (HTS).
Features:
- High Sensitivity and Extended Linearity: Detect
from 50 or fewer cells to several million cells with
excellent linearity.
- Simple: A single-step homogenous assay.
- Robust & Amenable to HTS: Stable luminescent
signal with excellent Z'-factor values for screening
applications.
Related Products:
See Section 6 of the catalog for a
complete list of D-luciferin products.
Reference:
1) J. Biolumin. Chemilumin. 10,
29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol.
Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991);
5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160,
81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986). |
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Catelog Number :
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30020-3 |
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Product Name :
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ATP-GloTM
Bioluminometric Cell Viability Assay Kit |
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Kit Components:
- ATP-GloTM substrate
- ATP-GloTM buffer
Description:
ATP-GloTM Cell Viability
Assay offers a homogenous assay for quantifying the number
of viable cells based on the presence of ATP, an indicator
of metabolically active cells. ATP-GloTM is based
on Firefly luciferase? requirement for ATP in producing
light from the oxidation reaction of D-luciferin (See Figure
B11). The assay is highly sensitive, detecting as little as
1 picomole of ATP. By relating the amount of ATP to the
number of viable cells, the assay has wide applications,
ranging from determinations of viable cell numbers, to cell
proliferation and to cytotoxicity. This assay can be
performed using a standard luminometer and is also
compatible with high-throughput screening (HTS).
Features:
- High Sensitivity and Extended Linearity: Detect
from 50 or fewer cells to several million cells with
excellent linearity.
- Simple: A single-step homogenous assay.
- Robust & Amenable to HTS: Stable luminescent
signal with excellent Z'-factor values for screening
applications.
Related Products:
See Section 6 of the catalog for a
complete list of D-luciferin products.
Reference:
1) J. Biolumin. Chemilumin. 10,
29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol.
Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991);
5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160,
81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986). |
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Catelog Number :
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30020-2 |
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Product Name :
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ATP-GloTM
Bioluminometric Cell Viability Assay Kit |
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Kit Components:
- ATP-GloTM substrate
- ATP-GloTM buffer
Description:
ATP-GloTM Cell Viability
Assay offers a homogenous assay for quantifying the number
of viable cells based on the presence of ATP, an indicator
of metabolically active cells. ATP-GloTM is based
on Firefly luciferase? requirement for ATP in producing
light from the oxidation reaction of D-luciferin (See Figure
B11). The assay is highly sensitive, detecting as little as
1 picomole of ATP. By relating the amount of ATP to the
number of viable cells, the assay has wide applications,
ranging from determinations of viable cell numbers, to cell
proliferation and to cytotoxicity. This assay can be
performed using a standard luminometer and is also
compatible with high-throughput screening (HTS).
Features:
- High Sensitivity and Extended Linearity: Detect
from 50 or fewer cells to several million cells with
excellent linearity.
- Simple: A single-step homogenous assay.
- Robust & Amenable to HTS: Stable luminescent
signal with excellent Z'-factor values for screening
applications.
Related Products:
See Section 6 of the catalog for a
complete list of D-luciferin products.
Reference:
1) J. Biolumin. Chemilumin. 10,
29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol.
Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991);
5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160,
81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986). |
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Catelog Number :
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30020-1 |
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Product Name :
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ATP-GloTM
Bioluminometric Cell Viability Assay Kit |
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Kit Components:
- ATP-GloTM substrate
- ATP-GloTM buffer
Description:
ATP-GloTM Cell Viability
Assay offers a homogenous assay for quantifying the number
of viable cells based on the presence of ATP, an indicator
of metabolically active cells. ATP-GloTM is based
on Firefly luciferase? requirement for ATP in producing
light from the oxidation reaction of D-luciferin (See Figure
B11). The assay is highly sensitive, detecting as little as
1 picomole of ATP. By relating the amount of ATP to the
number of viable cells, the assay has wide applications,
ranging from determinations of viable cell numbers, to cell
proliferation and to cytotoxicity. This assay can be
performed using a standard luminometer and is also
compatible with high-throughput screening (HTS).
Features:
- High Sensitivity and Extended Linearity: Detect
from 50 or fewer cells to several million cells with
excellent linearity.
- Simple: A single-step homogenous assay.
- Robust & Amenable to HTS: Stable luminescent
signal with excellent Z'-factor values for screening
applications.
Related Products:
See Section 6 of the catalog for a
complete list of D-luciferin products.
Reference:
1) J. Biolumin. Chemilumin. 10,
29(1995); 2) Anal. Biochem. 175, 14(1988); 3) Biotechnol.
Bioeng. 42, 30(1993); 4) J. Biolumin. Chemilumin. 6, 193(1991);
5) Biochem. J. 295, 165(1993); 6) J. Immunol. Methods 160,
81(1993); 7) J. Natl. Cancer Inst. 77, 1039(1986). |
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Catelog Number :
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30026 |
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Product Name :
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Calcein AM Cell
Viability Assay Kit
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Kit Components:
Calcein AM in anhydrous DMSO
Description:
Calcein AM is a widely used green
fluorescent cell marker. Calcein AM is membrane-permeant and
thus can be introduced into cells via incubation. Once
inside the cells, calcein AM, a nonfluorescent molecule
itself, is hydrolyzed by endogenous esterase into the highly
negatively charged green fluorescent calcein. The
fluorescent calcein is retained in the cytoplasm in live
cells. Calcein AM has been served as an excellent tool for
the studies of cell membrane integrity and for long-term
cell tracing. Calcein AM Cell Viability Assay Kit is
designed to quantify live cell numbers based on the presence
of their cytoplasmic membrane integrity. It is a true
end-point assay for cell viability. The fluorescent signal
is monitored using 485 nm excitation wavelength and 530 nm
emission wavelength. The fluorescent signal generated from
the assay is proportional to the number of living cells in
the sample (See Figure B10).
Features:
- True end-point viability assay: Detect only cells
with uncompromised cytoplasmic membrane integrity.
- Simple & Fast: A single 30-min assay.
Spectra:
See #80013 for spectra.
Related Products:
- Calcein, 100 mg (#80013)
- Calcein AM, 1mg (#80011)
- Calcein AM, 100 uL at 4 mM in DMSO (#80011-1)
- Calcein AM, 1mL at 1mg/mL in DMSO (#80011-2)
- Calcein AM, 20x50 ug (#80011-3)
Reference:
1) J. Neurosci. Methods. 50, 205(1993);
2) J. Immunol. Methods 163,181(1993); 3) Cancer Lett. 87,
199(1994); 4) J. Immunol. Methods 178, 41(1995)
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Catelog Number :
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30006 |
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Product Name :
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MTT Cell Viability
Assay Kit |
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Kit Components:
MTT solution
Description:
MTT Cell Viability Assay Kit provides
a simple method for determining live cell numbers using a
standard colorimetric plate readers. Determination of live
cell numbers is often used to assess the rate of cell
proliferation and cell cytotoxicity caused by drugs and
cytotoxic agents. Among all non-radioactive viability
assays, MTT assay developed by Mossman is one of the most
versatile and popular assays. MTT is a tetrazolium salt that
is turned into a purple formazan product after reduction by
mitochondrial enzymes that are only present in metabolically
active live cells, not in dead cells. The amount of formazan
product generated is proportional to the number of living
cells in the sample. The formazan product can be solubilized
and then photometrically quantified at 570 nm.
Features:
Simple: Ready-to-use MTT solution.
Flexible: Compatible with a standard
spectrophotometer or a photometric plate reader for high
throughput assays.
Related Products:
Reference:
1) J Immunol. Methods 174,
311(1994); 2) J Immunol. Methods 145, 199(1991); 3) J
Immunol. Methods 131, 165(1990); 4) Cancer Res. 48,
598(1988).
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Catelog Number :
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30027 |
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Product Name :
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Viability/Cytotoxicity Assay kit for Bacteria Live and Dead
Cells |
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Kit Components:
- 2 vials (100 uL/vial): DMAO, 5 mM
- 2 vials (150 uL/vial): Ethidium Homodimer-III
(EthD-III), 2 mM
Description:
This kit provides a two-color
fluorescence staining on both live bacteria (green) and dead
bacteria (red) using two probes DMAO and EtD-III. DMAO is a
green-fluorescent nucleic acid dye which stains both live
and dead bacteria with intact and damaged cell membranes.
EtD-III is a red-fluorescent nucleic acid dye that only
stains dead bacteria with damaged cell membranes. With an
appropriate mixture of DMAO and EtD-III, bacteria with
intact cell membranes stain fluorescent green, whereas
bacteria with damaged cell membranes stain fluorescent red.
The kit is suitable for use with fluorescence microscopes
and flow cytometers. The assay principles are general and
applicable to most bacteria types.
Features:
- Dual Detection: Detect live and dead bacteria
cells in a cell population simultaneously.
- Simple & Fast: 15 min dye loading and measure
without washing.
- Economic: Perform viability and cytotoxicity
assays at the same time.
- Versatile: Analysis compatiable with flow
cytometers and fluorescence microscopes using popular
settings for fluorescein and propidium iodide.
Related Products:
Viability/Cytotoxicity Staining Kit for
Mammalian Live & Dead Cells (Cat.# 30002)
Reference: |
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Catelog Number :
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30002 |
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Product Name :
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Viability/Cytotoxicity Staining Kit for Animal Live & Dead Cells
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Kit Components:
- 2 x 50 uL Calcein AM (4 mM)
- 2 x 150 uL EthD-III (2 mM)
Description:
Viability/cytotoxicity Staining Kit
for Animal Live & Dead Cells provides a two-color
fluorescent staining of live (green) and dead cells (red)
using two probes. Calcein AM stains live cells green while
EthD-III stains dead cells red. These probes measure two
recognized parameters of cell viability ? intracellular
esterase activity and plasma membrane integrity (1,2).
The kit is suitable for use with fluorescence microscopes,
fluorescence multi-well plate scanners and flow cytometers.
The assay principles are general and applicable to most
eukaryotic cell types, including adherent cells and certain
tissues, but not to bacteria or yeast. This
fluorescence-based method of assessing cell viability can be
used in place of trypan blue exclusion, 51Cr release and
similar methods for determining cell viability and
cytotoxicity. EthD-III (# 40050 and #40051) is a proprietary
DNA stain developed by Biotium and is a superior alternative
to EthD-I offered by our competitors. EthD-III has higher
DNA binding affinity and thus brighter fluorescence (70%
brighter than Ethidium homodimer I).
Features:
- Dual Detection: Detect both live and dead cells
simultaneously.
- Simple & Fast: Require only a 30-min dye loading
time and then measure without washing.
- Economical: Perform viability and cytotoxicity
assays at the same time.
- Versatile: Analyze with flow cytometers,
fluorescence microscopes or fluorescence plate readers.
Related Products:
1) Calcein AM, 1 mg (Cat. # 80011); 2)
Calcein AM, 4mM, 100 uL (Cat. # 80011-1); 3) Calcein AM, 1 mg/mL
(Cat. # 80011-2); 4) Calcein AM, 20 x 50 ug (Cat. # 80011-3); 5)
Ethidium Homodimer I (EthD-I or EtDi), 1mg (Cat. # 40010); 6)
EthD-I, 2 mM, 0.5 mL (Cat. # 40014 ); 7) EthD-III, 1mg (Cat. #
40050); 8) EthD-III, 1mM, 0.2 mL (Cat. # 40051)
Reference:
Biotechniques 29, 874-880 (2000) |
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Catelog Number :
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30007 |
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Product Name :
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XTT Cell Viability
Assay Kit |
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Kit Components:
XTT solution
Activation Reagent
Description:
XTT Cell Viability Assay Kit provides
a simple method for determining live cell numbers using
standard photometric microplate readers. Determination of
live cell numbers is often used to assess the rate of cell
proliferation and to screen cytotoxic agents. XTT is a
tetrazolium derivative. Similar to MTT, XTT measures cell
viability based on the activity of mitochondrial enzymes in
live cells that reduce XTT and are inactivated shortly after
cell death. Unlike MTT, XTT is reduced to a highly
water-soluble orange-colored product instead of the
insoluble formazan from MTT, thus eliminating the
solubilization step required for the MTT assay. The amount
of water-soluble product generated from XTT reduction is
proportional to the number of living cells in the sample and
can be photometrically quantified at 475 nm. Please see
Table B1 for comparison with other Viability/ Cytotoxicity
Assay Kits. environment while inhibition of growth maintains
an oxidized environment. Reduction related to growth causes
the REDOX indicator to change from oxidized (nonfluorescent,
blue) form to reduced (fluorescent, red) form. The
fluorescent signal is monitored using 530-560 nm excitation
wavelength and 590 nm emission wavelength. The absorbance is
monitored at 570 nm and 600 nm. The fluorescent or
colorimetric signal generated from the assay is proportional
to the number of living cells in the sample.
Features:
Simple: Homogenous assay using ready-to-use XTT and
activation reagent solutions
Kinetic Monitoring: Continuous color development
permitting measurements at multiple time points for extended
detection range.
Related Products:
Reference:
1) J. Infect. dis. 172,
1153(1995); 2) J Immunol. Methods 159, 81(1993); 3) J
Immunol. Methods 147, 153(1992); 4) J Immunol. Methods
142, 257(1991); 5) J. Natl. Cancer Inst. 81, 577(1989);
6) Cancer Res. 48, 4827(1988).
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