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Reporter Gene Assays
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Catelog Number :
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30005-2 |
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Product Name :
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Firefly & Renilla
Luciferase Assay Kit |
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Kit Components:
- 2x10 mg D-Luciferin
- 1 mL 100X Coelenterazine
- 30 mL 5X Passive Lysis Buffer
- 100 mL Firefly Luciferase Assay Buffer
- 50 mL Renilla Luciferase Assay Buffer
- 50 mL Renilla Luciferase Assay Enhancer
Description:
Firefly and Renilla
luciferases are widely used as reporter genes for studying
gene regulation and function, and for pharmaceutical
screening. Renilla Luciferase is often used in conjunction
with Firefly Luciferase as a normalizing transfection
control or for multiplex transcriptional reporter assays
(1, 2). As with many enzymes, Firefly
luciferase and Renilla luciferase follow
Michaelis-Menten kinetics and thus maximum light output is
not achieved until substrates (above the Km) and co-factor
are present in large excess. When assayed under these
conditions, light emitted from the reaction is directly
proportional to the number of luciferase enzyme molecules.
Biotium's Firefly & Renilla luciferase assay
kit is designed for detection and quantification of Firefly
and Renilla luciferase reporter enzymes from cultured cells
in a simple, efficient and linear fashion.
Features:
- Sensitivity and Linearity: Linear correlation
between reporter gene expression and light output for
transfection using 1 ng to 1 ug DNA of either Firefly or
Renilla luciferase reporter construct.
- Low Autoluminescence & High Sensitivity: Reduced
autoluminescence background for Renilla luciferase assay and
consequently increased sensitivity.
- Convenience: One kit for both luciferase assays.
Related Products:
Biotium offers a large number of
coelenterazine and D-luciferin products. For a complete list,
see Section 6E. Bioluminescent Enzyme Substrates.
Reference:
1). Carcinogenesis. 22 , 661-4
(2001) . 2). J Biomol Screen. 8 , 676-84 (2004) . |
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Catelog Number :
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30005-1 |
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Product Name :
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Firefly & Renilla
Luciferase Assay Kit |
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Kit Components:
- 2x1mg D-Luciferin
- 100 uL 100X Coelenterazine
- 10 mL 5X Passive Lysis Buffer
- 10 mL Firefly Luciferase Assay Buffer
- 10 mL Renilla Luciferase Assay Buffer
- 10 mL Renilla Luciferase Assay Enhancer
Description:
Firefly and Renilla
luciferases are widely used as reporter genes for studying
gene regulation and function, and for pharmaceutical
screening. Renilla Luciferase is often used in conjunction
with Firefly Luciferase as a normalizing transfection
control or for multiplex transcriptional reporter assays (1,
2). As with many enzymes, Firefly luciferase and
Renilla luciferase follow Michaelis-Menten kinetics and
thus maximum light output is not achieved until substrates
(above the Km) and co-factor are present in large excess.
When assayed under these conditions, light emitted from the
reaction is directly proportional to the number of
luciferase enzyme molecules. Biotium's Firefly &
Renilla luciferase assay kit is designed for detection
and quantification of Firefly and Renilla luciferase
reporter enzymes from cultured cells in a simple, efficient
and linear fashion.
Features:
- Sensitivity and Linearity: Linear correlation
between reporter gene expression and light output for
transfection using 1 ng to 1 ug DNA of either Firefly or
Renilla luciferase reporter construct.
- Low Autoluminescence & High Sensitivity: Reduced
autoluminescence background for Renilla luciferase assay and
consequently increased sensitivity.
- Convenience: One kit for both luciferase assays.
Related Products:
Biotium offers a large number of
coelenterazine and D-luciferin products. For a complete list,
see Section 6E. Bioluminescent Enzyme Substrates.
Reference:
1). Carcinogenesis. 22 , 661-4
(2001) . 2). J Biomol Screen. 8 , 676-84 (2004) . |
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Catelog Number :
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30003-2 |
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Product Name :
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Firefly Luciferase
Assay Kit |
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Kit Components:
- 2x10 mg D-luciferin
- 30 mL 5X Firefly luciferase lysis buffer
- 100 mL Firefly luciferase assay buffer
Description:
Firefly luciferase is widely
used as a reporter gene for studying gene regulation and
function, and for pharmaceutical screening (1). It is a very
sensitive genetic reporter due to the lack of any endogenous
luciferase activity in mammalian cells or tissues and the
very nature of bioluminescence (2). Firefly
luciferase is a 62,000 dalton protein which is active as a
monomer and does not require subsequent processing for its
activity. The enzyme catalyzes ATP-dependent D-luciferin
oxidation by oxygen into oxyluciferin with emission of light
centered on 560 nm. As with many enzymes, Firefly
luciferase follows Michaelis-Menten kinetics, and as a
result maximum light output is not achieved until the
substrates and co-factor are present in large excess. When
assayed under these conditions, light emitted from the
reaction is directly proportional to the number of
luciferase enzyme molecules. This Firefly lu ciferase
assay kit is designed for detection and quantification of
Firefly luciferase reporter enzyme from cultured cells
in a simple, efficient and linear fashion.
Features:
Sensitivity and Linearity: Linear correlation
between luciferase gene expression and light output for
transfection using 1 ng to 1 ug DNA of a Firefly luciferase
reporter construct
Convenience: Conveniently packaged substrate sizes
permitting you to run a variable number of assays (50 assays
at a time for #30003-1 or 500 assays at a time for #30003-2)
Related Products:
D-Luciferin, Potassium Salt (Cat. # 10101)
D-Luciferin, Sodium Salt (Cat. # 10102)
Reference:
1. Anal. Biochem. 219:169-181 (1994). 2.
Anal. Biochem. 175:5-13 (1988). |
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Catelog Number :
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30003-1 |
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Product Name :
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Firefly Luciferase
Assay Kit |
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Kit Components:
- 3x1 mg D-luciferin
- 15 mL 5X Firefly luciferase lysis buffer
- 15 mL Firefly luciferase assay buffer
Description:
Firefly luciferase is widely
used as a reporter gene for studying gene regulation and
function, and for pharmaceutical screening (1). It is a very
sensitive genetic reporter due to the lack of any endogenous
luciferase activity in mammalian cells or tissues and the
very nature of bioluminescence (2). Firefly
luciferase is a 62,000 dalton protein which is active as a
monomer and does not require subsequent processing for its
activity. The enzyme catalyzes ATP-dependent D-luciferin
oxidation by oxygen into oxyluciferin with emission of light
centered on 560 nm. As with many enzymes, Firefly
luciferase follows Michaelis-Menten kinetics, and as a
result maximum light output is not achieved until the
substrates and co-factor are present in large excess. When
assayed under these conditions, light emitted from the
reaction is directly proportional to the number of
luciferase enzyme molecules. This Firefly lu ciferase
assay kit is designed for detection and quantification of
Firefly luciferase reporter enzyme from cultured cells
in a simple, efficient and linear fashion.
Features:
Sensitivity and Linearity: Linear correlation
between luciferase gene expression and light output for
transfection using 1 ng to 1 ug DNA of a Firefly luciferase
reporter construct
Convenience: Conveniently packaged substrate sizes
permitting you to run a variable number of assays (50 assays
at a time for #30003-1 or 500 assays at a time for #30003-2)
Related Products:
D-Luciferin, Potassium Salt (Cat. # 10101)
D-Luciferin, Sodium Salt (Cat. # 10102)
Reference:
1. Anal. Biochem. 219:169-181 (1994). 2.
Anal. Biochem. 175:5-13 (1988). |
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Catelog Number :
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30004-2 |
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Product Name :
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Renilla Luciferase
Assay Kit |
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Kit Components:
- 1mL 100X Coelenterazine
- 30 mL 5X Renilla Luciferase Lysis Buffer
- 50 mL Renilla Luciferase Assay Buffer
- 50 mL Renilla Luciferase Assay Enhancer
Description:
Renilla luciferase has been
used as a reporter gene for studying gene regulation and
function in vitro and in vivo. Recently, Renilla luciferase
has been widely used in multiplex transcriptional reporter
assays or as a normalizing transfection control for Firefly
luciferase assay (1, 2). Renilla
luciferase, a monomeric 36,000 Dalton protein, catalyzes
coelenterazine oxidation by oxygen to produce light. The
enzyme does not require post-translational modification for
its activity, and may function as a genetic reporter
immediately following translation. Coelenterazine native is
the natural substrate for Renilla luciferase. However, over
a dozen of coelenterazine analogs have been synthesized,
many of which are now commercially available from Biotium.
These coelenterazine analogs all function as substrates for
Renilla luciferase with different properties in terms of
emission wavelength, cell membrane permeability and quantum
efficiency. Coelenterazine also emits light from
enzyme-independent oxidation, a process known as
autoluminescence. The autoluminescence is enhanced by
superoxide anion and peroxynitrite in cells and tissues.
Biotium's Renilla Luciferase Assay Kit is designed to
provide a simple and sensitive method of detecting Renilla
luciferase. Through the use of a specially designed
coelenterazine derivative and buffer formulation, this assay
kit yields reliable, linear results with minimal
autoluminescence background and superior sensitivity.
Features:
- Sensitivity and Linearity: Linear correlation
between luciferase gene expression and light output for
transfection using 1 ng to 1 ug DNA of a Firefly luciferase
reporter construct
- Convenience: Conveniently packaged substrate
sizes permitting you to run a variable number of assays (50
assays at a time for #30003-1 or 500 assays at a time for
#30003-2 )
Related Products:
Biotium offers a large number of
coelenterazine products. For a complete list, see Section 6E.
Bioluminescent Enzyme Substrates.
Reference:
1. Carcinogenesis. 22 , 661-4 (2001) .
2. J Biomol Screen. 8 , 676-84 (2004) . |
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Catelog Number :
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30004-1 |
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Product Name :
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Renilla Luciferase
Assay Kit |
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Kit Components:
- 150 uL 100X Coelenterazine
- 10 mL 5X Renilla Luciferase Lysis Buffer
- 10 mL Renilla Luciferase Assay Buffer
- 10 mL Renilla Luciferase Assay Enhancer
Description:
Renilla luciferase has been
used as a reporter gene for studying gene regulation and
function in vitro and in vivo. Recently, Renilla luciferase
has been widely used in multiplex transcriptional reporter
assays or as a normalizing transfection control for Firefly
luciferase assay (1, 2). Renilla
luciferase, a monomeric 36,000 Dalton protein, catalyzes
coelenterazine oxidation by oxygen to produce light. The
enzyme does not require post-translational modification for
its activity, and may function as a genetic reporter
immediately following translation. Coelenterazine native is
the natural substrate for Renilla luciferase. However, over
a dozen of coelenterazine analogs have been synthesized,
many of which are now commercially available from Biotium.
These coelenterazine analogs all function as substrates for
Renilla luciferase with different properties in terms of
emission wavelength, cell membrane permeability and quantum
efficiency. Coelenterazine also emits light from
enzyme-independent oxidation, a process known as
autoluminescence. The autoluminescence is enhanced by
superoxide anion and peroxynitrite in cells and tissues.
Biotium's Renilla Luciferase Assay Kit is designed to
provide a simple and sensitive method of detecting Renilla
luciferase. Through the use of a specially designed
coelenterazine derivative and buffer formulation, this assay
kit yields reliable, linear results with minimal
autoluminescence background and superior sensitivity.
Features:
- Sensitivity and Linearity: Linear correlation
between luciferase gene expression and light output for
transfection using 1 ng to 1 ug DNA of a Firefly luciferase
reporter construct
- Convenience: Conveniently packaged substrate
sizes permitting you to run a variable number of assays (50
assays at a time for #30003-1 or 500 assays at a time for
#30003-2 )
Related Products:
Biotium offers a large number of
coelenterazine products. For a complete list, see Section 6E.
Bioluminescent Enzyme Substrates.
Reference:
1. Carcinogenesis. 22 , 661-4 (2001) .
2. J Biomol Screen. 8 , 676-84 (2004) . |
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Catelog Number :
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30028-3 |
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Product Name :
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Steady-Luc
Firefly HTS Assay Kit
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Kit Components:
- 10 vial of D-Luciferin
- 10 bottle of 10 mL Steady-Luc Firefly Assay
Buffer
Description:
Firefly luciferase is widely
used as a reporter for studying gene regulation and
function, and for pharmaceutical screening. The enzyme
catalyzes ATP-dependent D-luciferin oxidation by oxygen into
oxyluciferin with emission of light centered on
560nm.However, the Light production resulting from the
reaction leads to formation of suicidal adenyloxyluciferin
at the enzyme surface. It results in very short half-life of
the light emission with a flash-type kinetics. Several
substances have been described to prolong light production
by regenerating enzyme through removing inhibitory
oxyluciferin from the enzyme surface. But the duration
(10-15 min) is still too short for batch process screening.
The Biotium?s Steady-luc HTS assay system is a proprietary
mixture of substances that modify the enzymatic reaction to
produce a long lasting signal by preventing the formation of
the adenyl-oxyluciferin at the enzyme surface. It is a
homogeneous high sensitivity firefly luciferase reporter
gene assay kit with a half-life of 3-5 hours for the
quantification of firefly luciferase expression in mammalian
cells. This kit is specifically designed for batch
processing systems using high-density microplates such as
384- and 1536-well plates, in high throughput environments.
Features:
- Simple: single step homogeneous assay.
- High sensitivity: higher sensitivity than similar
products offered by competitors.
- Economic: save up to 45% compared to other
brands.
Related Products:
Firefly Luciferase Assay Kit (cat.#
30003-1 & 30003-2 )
Reference: |
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Catelog Number :
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30028-2 |
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Product Name :
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Steady-Luc
Firefly HTS Assay Kit
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Kit Components:
- 1 vial of D-Luciferin
- 1 bottle of 100 mL Steady-Luc Firefly Assay
Buffer
Description:
Firefly luciferase is widely
used as a reporter for studying gene regulation and
function, and for pharmaceutical screening. The enzyme
catalyzes ATP-dependent D-luciferin oxidation by oxygen into
oxyluciferin with emission of light centered on
560nm.However, the Light production resulting from the
reaction leads to formation of suicidal adenyloxyluciferin
at the enzyme surface. It results in very short half-life of
the light emission with a flash-type kinetics. Several
substances have been described to prolong light production
by regenerating enzyme through removing inhibitory
oxyluciferin from the enzyme surface. But the duration
(10-15 min) is still too short for batch process screening.
The Biotium?s Steady-luc HTS assay system is a proprietary
mixture of substances that modify the enzymatic reaction to
produce a long lasting signal by preventing the formation of
the adenyl-oxyluciferin at the enzyme surface. It is a
homogeneous high sensitivity firefly luciferase reporter
gene assay kit with a half-life of 3-5 hours for the
quantification of firefly luciferase expression in mammalian
cells. This kit is specifically designed for batch
processing systems using high-density microplates such as
384- and 1536-well plates, in high throughput environments.
Features:
- Simple: single step homogeneous assay.
- High sensitivity: higher sensitivity than similar
products offered by competitors.
- Economic: save up to 45% compared to other
brands.
Related Products:
Firefly Luciferase Assay Kit (cat.#
30003-1 & 30003-2 )
Reference: |
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Catelog Number :
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30028-1 |
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Product Name :
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Steady-Luc
Firefly HTS Assay Kit
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Kit Components:
- 1 vial of D-Luciferin
- 1 bottle of 10 mL Steady-Luc Firefly Assay Buffer
Description:
Firefly luciferase is widely
used as a reporter for studying gene regulation and
function, and for pharmaceutical screening. The enzyme
catalyzes ATP-dependent D-luciferin oxidation by oxygen into
oxyluciferin with emission of light centered on
560nm.However, the Light production resulting from the
reaction leads to formation of suicidal adenyloxyluciferin
at the enzyme surface. It results in very short half-life of
the light emission with a flash-type kinetics. Several
substances have been described to prolong light production
by regenerating enzyme through removing inhibitory
oxyluciferin from the enzyme surface. But the duration
(10-15 min) is still too short for batch process screening.
The Biotium?s Steady-luc HTS assay system is a proprietary
mixture of substances that modify the enzymatic reaction to
produce a long lasting signal by preventing the formation of
the adenyl-oxyluciferin at the enzyme surface. It is a
homogeneous high sensitivity firefly luciferase reporter
gene assay kit with a half-life of 3-5 hours for the
quantification of firefly luciferase expression in mammalian
cells. This kit is specifically designed for batch
processing systems using high-density microplates such as
384- and 1536-well plates, in high throughput environments.
Features:
- Simple: single step homogeneous assay.
- High sensitivity: higher sensitivity than similar
products offered by competitors.
- Economic: save up to 45% compared to other
brands.
Related Products:
Firefly Luciferase Assay Kit (cat.#
30003-1 & 30003-2 )
Reference: |
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+32 (0) 16
58 90 45 
+32 (0) 16
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