Biotium in collaboration with AlleLogic
Biosciences Corp. has developed EvaGreenTM as a superior
fluorescent DNA stain for quantitative real-time PCR (qPCR). EvaGreenTM
is truely a remarkable dye in many aspects. Upon binding to DNA, the
fluorescence of EvaGreenTM is several-fold higher than that
of SYBR Green I while the dye shows very little inhibition to the PCR
process. Unlike SYBR Green, which has been reported to be unstable,
EvaGreenTM is highly robust, both thermally and
hydrolytically under alkaline or acidic condition. In addition, the
absorption and emission spectra of EvaGreenTM are similar
to those of SYBR Green I or FAM, which means that the same optical
setting for SYBR Green I can also be used for EvaGreenTM.
We now offer two EvaGreenTM products: 1) Eva GreenTM
at 20x concentration (#31000); and 2) EvaGreenTM qPCR Basic
Mix at 2x concentration (#31001). The Basic Mix
contains everything you need to run a qPCR except for the Taq
enzyme(available in May). We will soon offer EvaGreenTM
qPCR Master Mix.
EvaGreen
TM is not just another qPCR dye. It is simply
the best. For details,
please download the flyer here.
Features:
- Very Little PCR inhibition
Unlike SYBR Green, EvaGreenTM shows very little PCR
inhibition.
- Highly bright fluorescence
The fluorescence brightness of EvaGreenTM in qPCR is
simply unmatched by that of SYBR Green I.
- Superior stability
Unlike SYBR Green, which has been reported to be unstable,
EvaGreenTM is extremely stable.
- Excellent compatibility
Simply replace SYBR Green with EvaGreenTM. No need to
change anything else in your existing formulation and instrument
setting. The only difference you will see is a better performance.
Related Products:
Reference:
SYBR is a trademark of Molecular Probes, Inc.
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